DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins

Nature Biotechnology

Volumn 33, Issue 11, 2015, Pages 1162-1164

  Woo, Je Wook ^a   Kim, Jungeun ^b,c   Kwon, Soon Il ^a   Corvalán, Claudia ^c   Cho, Seung Woo ^c,e   Kim, Hyeran ^b   Kim, Sang Gyu ^b   Kim, Sang Tae ^b   Choe, Sunghwa ^a,c,d   Kim, Jin Soo ^b,c  

^a Seoul National University   (South Korea)

^b Institute for Basic Science   (South Korea)

^c Seoul National University   (South Korea)

^d Seoul National University   (South Korea)

^e STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GENES; NUCLEIC ACIDS; PLANTS (BOTANY);

ARABIDOPSIS THALIANA; GENETIC VARIATION; GERMLINE; NATURALLY OCCURRING; PLANT GENOMES; PLANT PROTOPLASTS; RIBONUCLEOPROTEINS;

GENETICALLY MODIFIED PLANTS;

CAS9 PROTEIN; CELLULAR APOPTOSIS SUSCEPTIBILITY PROTEIN; CRISPR ASSOCIATED PROTEIN; GENOMIC DNA; GUIDE RNA; RIBONUCLEOPROTEIN; UNCLASSIFIED DRUG; VEGETABLE PROTEIN;

ARABIDOPSIS THALIANA; ARTICLE; CRISPR CAS SYSTEM; GENE DELETION; GENE FREQUENCY; GENE INSERTION; GENE TARGETING; GENETIC TRANSFECTION; GENETIC VARIABILITY; GERMLINE MUTATION; HUMAN; HUMAN CELL; IN VITRO STUDY; LETTUCE; MUTAGENESIS; NONHUMAN; PLANT GENOME; PLANT REGENERATION; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN PURIFICATION; PROTOPLAST; RICE; RNA EDITING; TOBACCO; ARABIDOPSIS; GENE INACTIVATION; GENETIC ENGINEERING; GENETICS; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; ORYZA; PHYSIOLOGY; PROCEDURES; TRANSGENIC PLANT;

ARABIDOPSIS; BASE SEQUENCE; CRISPR-CAS SYSTEMS; GENE KNOCKOUT TECHNIQUES; GENETIC ENGINEERING; GENOME, PLANT; MOLECULAR SEQUENCE DATA; ORYZA; PLANT PROTEINS; PLANTS, GENETICALLY MODIFIED; PROTOPLASTS; RIBONUCLEOPROTEINS; TOBACCO;

EID: 84947255513     PISSN: 10870156     EISSN: 15461696     Source Type: Journal    
DOI: 10.1038/nbt.3389     Document Type: Article

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