The role of p21cip1 in adaptation of CHO cells to suspension and protein-free culture

Journal of Biotechnology

Volumn 130, Issue 3, 2007, Pages 282-290

  Astley, Kelly ^a   Naciri, Mariam ^b   Racher, Andrew ^c   Al Rubeai, Mohamed ^a  

^a UNIVERSITY OF BIRMINGHAM   (United Kingdom)

^b UNIVERSITY COLLEGE DUBLIN   (Ireland)

^c LONZA AG   (Switzerland)

Author keywords

Anchorage dependent; Apoptosis; Cell cycle; p21cip1; Proliferation; Protein free

Indexed keywords

ANTIBODIES; BLOOD; CELL CULTURE; CELL DEATH; CELL GROWTH; HYDRODYNAMICS;

ANCHORAGE DEPENDENT; HYDRODYNAMIC FORCES; PROLIFERATION; REGULATORY PROCESSES;

ENZYME INHIBITION;

CYCLIN DEPENDENT KINASE INHIBITOR 1; MONOCLONAL ANTIBODY; CYCLIN DEPENDENT KINASE INHIBITOR 1A;

ADAPTATION; ANIMAL CELL; ANTIBODY PRODUCTION; ARTICLE; CELL ADHESION; CELL AGGREGATION; CELL CULTURE; CELL GROWTH; CELL SURVIVAL; CELL SUSPENSION; CHO CELL; CONTROLLED STUDY; CULTURE MEDIUM; HYDRODYNAMICS; NONHUMAN; PRIORITY JOURNAL; PRODUCTIVITY; PROTEIN DETERMINATION; PROTEIN EXPRESSION; ANIMAL; CELL CYCLE; CELL LINE; CRICETULUS; CULTURE TECHNIQUE; DRUG EFFECT; GENE EXPRESSION; HAMSTER; HUMAN; METABOLISM; MOUSE; TIME;

ADAPTATION, PHYSIOLOGICAL; ANIMALS; CELL AGGREGATION; CELL CULTURE TECHNIQUES; CELL CYCLE; CELL LINE; CHO CELLS; CRICETINAE; CRICETULUS; CULTURE MEDIA, SERUM-FREE; CYCLIN-DEPENDENT KINASE INHIBITOR P21; GENE EXPRESSION; HUMANS; MICE; TIME FACTORS;

EID: 34250379089     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2007.04.012     Document Type: Article

References (33)

1. Abdul Majid F.A., and Al-Rubeai M. The mechanical strength of mammalian cells during mitotic cell division. In: Carrondo M.J.T., Griffiths B., and Moreira L.P. (Eds). Animal Cell Technology (1997), Kluwer Academic Publishers 731-736
2. Al-Rubeai M., Emery A.N., and Chalder S. The effect of Pluronic F-68 on hybridoma cells in continuous culture. Appl. Microbiol. Biotechnol. 37 (1992) 44-45
3. Al-Rubeai M., Singh R.P., Emery A.N., and Zhang Z. Cell cycle and cell size dependence of susceptibility to hydrodynamic forces. Biotechnol. Bioeng. 46 (1995) 88-92
4. Assoian R.K. Anchorage-dependent cell cycle progression. J. Cell Biol. 136 (1997) 1-4
5. Bebbington C.R., Renner G., Thomson S., King D., Abrahms D., and Yarranton G.T. High-level expression of a recombinant antibody from myeloma cells using a glutamine synthetase gene as an amplifiable selectable marker. Bio/Technology 10 (1992) 169-175
6. Bi J.-X., Shuttleworth J., and Al-Rubeai M. Uncoupling of cell growth and proliferation results in enhancement of productivity in p21-arrested CHO cells. Biotechnol. Bioeng. 85 (2004) 741-749
7. Croughan M.S., Sayre E.S., and Wang D.I. Viscous reduction of turbulent damage in animal cell culture. Biotechnol. Bioeng. 33 (1989) 862-872
8. Eckhart L., Reinisch C., Inoue S., Messner P., Dockal M., Mayer C., and Tschachler E. A basement membrane-like matrix formed by cell-released proteins at the medium/air interface supports growth of keratinocytes European. J. Cell Biol. 82 11 (2003) 549-555
9. Fussenegger M., Schlatter S., Datwyler D., Mazur X., and Bailey J.E. Controlled proliferation by multigene metabolic engineering enhances the productivity of Chinese hamster ovary cells. Nat. Biotechnol. 16 5 (1998) 416-417
10. Handa-Corrigan A., Emery A.N., and Spier R.E. Effects of gas-liquid interfaces on the growth of suspended mammalian cells: mechanisms of cell damage by bubbles. Enzyme Microbiol. Technol. 11 (1989) 230-235
11. Hofseth L.J. The adaptive imbalance to genotoxic stress: genome guardians rear their ugly heads. Carcinogenesis 25 (2004) 1787-1793
12. Hu W.S., and Wang D.I.C. Mammalian cell culture technology: a review from an engineering prespective. In: Thilly W.G. (Ed). Mammalian Cell Technology (1986), Butterworths, Massachusetts 167-197
13. Ibara N., Watanabe S., Bi J.-X., Shuttleworth J., and Al-Rubeai M. Modulation of cell cycle for enhancement of antibody productivity in perfusion culture of NS0 cells. Biotechnol. Prog. 19 (2003) 224-228
14. Jordan M., Eppenberger H.M., Sucker H., Widmer F., and Einsele A. Interactions between animal cells and gas bubbles: the influence of serum and pluronic F68 on the physical properties of the bubble surface. Biotechnol. Bioeng. 43 (1994) 446-454
15. Keane, and Julian T. Effect of shear stress on expression of a recombinant protein by Chinese hamster ovary cells. Biotechnol. Bioeng. 81 2 (2003)
16. Keen M.J., and Steward T.W. Adaptation of cholesterol-requiring NS0 mouse myeloma cells to high density growth in a fully defined protein-free and cholesterol-freeculture medium. Cyotechnology 17 (1995) 203-211
17. Kilburn D.G., and Webb F.C. The cultivation of animal cells at controlled dissolved oxygen partial pressure. Biotechnol. Bioeng. 10 (1968) 801-814
18. Kovar J., and Franek F. Serum-free medium for hybridoma and parental myeloma cell cultivation: a novel composition of growth-supporting substances. Immunol. Lett. 7 6 (1984) 339-345
19. Kunas K.T., and Papoutsakis E.T. The protective effect of serum against hydrodynamic damage of hybridoma cells in agitated and surface-aerated bioreactors. J. Biotechnol. 15 (1990) 57-69
20. Kunas K.T., and Papoutsakis E.T. Damage mechanisms of suspended animal cells in agitated bioreactors with and without bubble entrainment. Biotechnol. Bioeng. 36 5 (1990) 476-483
21. Ludwig, and Andreas. Influence of shear stress on adherent mammalian cells during division. Biotechnol. Lett. 14 10 (1992)
22. McQueen A., and Bailey J.E. Influence of serum level, cell line, flow type and viscosity on flow-induced lysis of suspended mammalian cells. Biotechnol. Lett. 11 8 (1989) 531-536
23. Mueller S., Cadenas E., and Schonthal A.H. p21WAF1 regulates anchorage-independent growth of HCT116 colon carcinoma cells via E-cadherin expression. Cancer Res. 60 (2000) 156-163
24. Oh S.K.W., Nienow A.W., Al-Rubeai M., and Emery A.N. The effects of agitation intensity with and without continuous sparging on the growth and antibody production of hybridoma cells. J. Biotechnol. 12 (1989) 45-62
25. Ozturk S., Kaseko G., Mahaworasilpa T., and Coster H.G.L. Hybrid. Hybridomics 22 4 (2003) 267-272
26. Peterson J.F., McIntire L.V., and Papoutsakis E.T. Shear sensitivity of cultured hybridoma cells (CRL 8018) depends on mode of growth, culture age and metabolite concentration. J. Biotechnol. 7 (1988) 229-246
27. Radford K., Niloperbowo W., Reid S., and Greenfield P.F. Weaning of three hybridoma cell lines to serum free low protein medium. Cytotechnology 6 (1991) 65-78
28. Renner W.A., Jordan M., Eppenberger H.M., and Leist C. Cell-cell adhesion and aggregation: influence on the growth behavior of CHO cells. Biotechnol. Bioeng. 41 (1993) 188-193
29. Roninson I.B. Oncogenic functions of tumour suppressor p21 (Waf1/Cip1/Sdi1): association with cell senescence and tumour-promoting activities of stromal fibroblasts. Cancer Lett. 179 1-14 (2002)
30. Sinacore M., Drapeau D., and Adamson S.R. Adaptation of mammalian cells to growth in serum free media. In: Jenkins N. (Ed). Animal Cell Biotechnology. Methods in Biotechnology vol. 8 (1999), Humana Press, NJ 11-22
31. van der Pol L., Bakker W.A.M., and Tramper J. Effect of low serum concentrations (0-2.5%) on growth, production, and shear sensitivity of hybridoma cells. Biotechnol. Bioeng. 40 (1992) 179-182
32. Watanabe S., Shuttleworth J., and Al-Rubeai M. Regulation of cell cycle and productivity in NS0 cells by the over-expression of p21CIP1. Biotechnol. Bioeng. 77 (2002) 1-7
33. Weinert T.A., and Hartwell L.H. The RAD9 gene controls the cell cycle response to DNA damage in Saccharomyces cerevisiae. Science 241 (1988) 317-322