Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch

Nature Biotechnology

Volumn 34, Issue 6, 2016, Pages 646-651

  Oakes, Benjamin L ^a   Nadler, Dana C ^a   Flamholz, Avi ^a   Fellmann, Christof ^a   Staahl, Brett T ^a   Doudna, Jennifer A ^a,b   Savage, David F ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b LAWRENCE BERKELEY NATIONAL LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BINS; CHEMICAL ACTIVATION; LIGANDS; NUCLEIC ACIDS; PROTEINS;

ESTROGEN RECEPTOR; GENOME MODIFICATION; INSERTIONAL MUTAGENESIS; LIGAND BINDING DOMAIN; ONE-COMPONENT SYSTEMS; RAPID GENERATIONS; STREPTOCOCCUS PYOGENES; STRUCTURAL CONSTRAINTS;

BINDING SITES;

CASPASE 9; CRISPR ASSOCIATED PROTEIN; ESTROGEN RECEPTOR ALPHA; PROTEIN BINDING;

ALLOSTERISM; ANIMAL EXPERIMENT; ARTICLE; BINDING AFFINITY; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CONTROLLED STUDY; CRYSTAL STRUCTURE; DNA BINDING; EUKARYOTIC CELL; HUMAN; HUMAN CELL; IN VITRO STUDY; LIGAND BINDING; MOUSE; MUTAGENESIS; NONHUMAN; NUCLEAR LOCALIZATION SIGNAL; PDZ DOMAIN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN ENGINEERING; PROTEIN FUNCTION; BINDING SITE; GENE EXPRESSION REGULATION; GENETICS; PROCEDURES; PROTEIN DOMAIN; REGULATOR GENE; SITE DIRECTED MUTAGENESIS;

ALLOSTERIC REGULATION; BINDING SITES; CASPASE 9; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; GENES, SWITCH; MUTAGENESIS, INSERTIONAL; MUTAGENESIS, SITE-DIRECTED; PROTEIN BINDING; PROTEIN DOMAINS; PROTEIN ENGINEERING;

EID: 84974602586     PISSN: 10870156     EISSN: 15461696     Source Type: Journal    
DOI: 10.1038/nbt.3528     Document Type: Article

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