Guide RNA functional modules direct Cas9 activity and orthogonality

Molecular Cell

Volumn 56, Issue 2, 2014, Pages 333-339

  Briner, Alexandra E ^a   Donohoue, Paul D ^b   Gomaa, Ahmed A ^c,d   Selle, Kurt ^a   Slorach, Euan M ^b   Nye, Christopher H ^b   Haurwitz, Rachel E ^b   Beisel, Chase L ^c   May, Andrew P ^b   Barrangou, Rodolphe ^a  

^a NORTH CAROLINA STATE UNIVERSITY   (United States)

^b Caribou Biosciences Inc   (United States)

^c North Carolina State University   (United States)

^d CAIRO UNIVERSITY   (Egypt)

Author keywords

[No Author keywords available]

Indexed keywords

CAS9 ENDONUCLEASE; CELLULAR APOPTOSIS SUSCEPTIBILITY PROTEIN; CELLULAR APOPTOSIS SUSCEPTIBILITY PROTEIN 9; DOUBLE STRANDED DNA; ENDONUCLEASE; GUIDE RNA; UNCLASSIFIED DRUG; CASPASE 9; DNA;

AMINO ACID SEQUENCE; ARTICLE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; COMPLEX FORMATION; CONSENSUS SEQUENCE; CONTROLLED STUDY; CRISPR CAS SYSTEM; DNA CLEAVAGE; DNA HAIRPIN; DNA TEMPLATE; ENZYME ACTIVITY; ENZYME SPECIFICITY; ESCHERICHIA COLI; GENE TARGETING; HEK293 CELL LINE; HUMAN; HUMAN CELL; IN VITRO STUDY; MOLECULAR GENETICS; NONHUMAN; ORTHOGONALITY; PHYLOGENETIC TREE; PROTEIN FUNCTION; PROTEIN RNA BINDING; PROTEIN SECONDARY STRUCTURE; PROTEIN TARGETING; RNA FOLDING; STOICHIOMETRY; STREPTOCOCCUS PYOGENES; TIGHT JUNCTION; CHEMISTRY; CONFORMATION; GENETIC ENGINEERING; GENETICS; PROCEDURES; ULTRASTRUCTURE;

CASPASE 9; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; DNA; DNA CLEAVAGE; GENETIC ENGINEERING; HEK293 CELLS; HUMANS; NUCLEIC ACID CONFORMATION; RNA, GUIDE;

EID: 84922322005     PISSN: 10972765     EISSN: 10974164     Source Type: Journal    
DOI: 10.1016/j.molcel.2014.09.019     Document Type: Article

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