Multiplexed pancreatic genome engineering and cancer induction by transfection-based CRISPR/Cas9 delivery in mice

Nature Communications

Volumn 7, Issue , 2016, Pages

  Maresch, Roman ^a,b   Mueller, Sebastian ^a   Veltkamp, Christian ^a   Öllinger, Rupert ^a   Friedrich, Mathias ^c   Heid, Irina ^a   Steiger, Katja ^a   Weber, Julia ^a,b   Engleitner, Thomas ^a,b   Barenboim, Maxim ^a,b   Klein, Sabine ^a   Louzada, Sandra ^c   Banerjee, Ruby ^c   Strong, Alexander ^c   Stauber, Teresa ^a,b   Gross, Nina ^a,b   Geumann, Ulf ^a,b   Lange, Sebastian ^a   Ringelhan, Marc ^a,d   Varela, Ignacio ^e   Unger, Kristian ^f   Yang, Fengtang ^c   Schmid, Roland M ^a   Vassiliou, George S ^c   Braren, Rickmer ^a   Schneider, Günter ^a   Heikenwalder, Mathias ^b,d   Bradley, Allan ^c   Saur, Dieter ^a,b   Rad, Roland ^a,b   more..

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^b GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

^c WELLCOME TRUST SANGER INSTITUTE   (United Kingdom)

^d TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^e University of Cantabria   (Spain)

^f INSTITUTE OF EPIDEMIOLOGY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BRCA2 PROTEIN; CASPASE 3; CASPASE 9; CYCLIN DEPENDENT KINASE INHIBITOR 2A; CYTOKERATIN 19; GREEN FLUORESCENT PROTEIN; PANCREAS SPECIFIC TRANSCRIPTION FACTOR 1A; SMAD4 PROTEIN; TRANSCRIPTION FACTOR; UNCLASSIFIED DRUG; UVOMORULIN; BRCA2 PROTEIN, MOUSE; KRAS2 PROTEIN, MOUSE; PROTEIN P21;

CANCER; CELLS AND CELL COMPONENTS; CHROMOSOME; DIGESTIVE SYSTEM; GENE; GENETIC ENGINEERING; GENOME; MUTATION; PHYLOGENETICS; RODENT; TUMOR; VULNERABILITY;

ACINAR CELL; ADULT; ALLELE; ANEUPLOIDY; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; APOPTOSIS; ARTICLE; CANCER SIZE; CARCINOGENESIS; CELL COUNT; CELL FRACTIONATION; CELL INFILTRATION; CELL LOSS; CELL SURVIVAL; CELL VACUOLE; CHROMOSOME DELETION; CHROMOSOME MUTATION; CHROMOSOME NUMBER; CHROMOSOME TRANSLOCATION; CONTROLLED STUDY; CRISPR CAS SYSTEM; CRISPR CAS9 SYSTEM; ELECTROPORATION; ENZYME INHIBITION; GENE DELETION; GENE INACTIVATION; GENE INSERTION; GENE INTERACTION; GENE LOCUS; GENE TARGETING; GENETIC ANALYSIS; GENETIC ENGINEERING; GENETIC RECOMBINATION; GENETIC SCREENING; GENETIC TRANSFECTION; GENETIC VARIABILITY; GENOME; GENOME EDITING; IN VITRO STUDY; IN VIVO STUDY; INDEL MUTATION; INDUCED MUTATION; INFLAMMATION; LIVER METASTASIS; MACROPHAGE; METAPLASIA; MOUSE; NEUTROPHIL; NONHUMAN; ONCOGENE K RAS; PANCREAS CANCER; PANCREAS CELL; PANCREATIC CANCER CELL LINE; PANCREATIC TISSUE; PHENOTYPIC VARIATION; PHYLOGENY; PROTEIN ANALYSIS; PROTEIN EXPRESSION; RECIPROCAL CHROMOSOME TRANSLOCATION; RNA CLEAVAGE; RNA SEQUENCE; TISSUE INJURY; TUMOR DIFFERENTIATION; TUMOR SUPPRESSOR GENE; ANIMAL; DNA SEQUENCE; EXPERIMENTAL NEOPLASM; GENE TRANSLOCATION; GENETICS; HIGH THROUGHPUT SEQUENCING; IMMUNOHISTOCHEMISTRY; METABOLISM; MUTATION; NUCLEAR MAGNETIC RESONANCE IMAGING; PANCREAS; PANCREAS TUMOR; POLYMERASE CHAIN REACTION; PROCEDURES;

MUS;

ANIMALS; BRCA2 PROTEIN; CARCINOGENESIS; CHROMOSOME DELETION; CRISPR-CAS SYSTEMS; ELECTROPORATION; GENETIC ENGINEERING; GENOME; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; IMMUNOHISTOCHEMISTRY; MAGNETIC RESONANCE IMAGING; MICE; MUTATION; NEOPLASMS, EXPERIMENTAL; PANCREAS; PANCREATIC NEOPLASMS; PHYLOGENY; POLYMERASE CHAIN REACTION; PROTO-ONCOGENE PROTEINS P21(RAS); SEQUENCE ANALYSIS, DNA; TRANSFECTION; TRANSLOCATION, GENETIC;

EID: 84959295529     PISSN: None     EISSN: 20411723     Source Type: Journal    
DOI: 10.1038/ncomms10770     Document Type: Article

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