Engineering and characterizing monomeric fluorescent proteins for live-cell imaging applications

Nature Protocols

Volumn 9, Issue 4, 2014, Pages 910-928

  Ai, Hui Wang ^a   Baird, Michelle A ^b   Shen, Yi ^c   Davidson, Michael W ^b   Campbell, Robert E ^c  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b FLORIDA STATE UNIVERSITY   (United States)

^c UNIVERSITY OF ALBERTA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENT DYE; MONOMERIC FLUORESCENT PROTEIN; OLIGONUCLEOTIDE; PEPTIDES AND PROTEINS; UNCLASSIFIED DRUG; GREEN FLUORESCENT PROTEIN; PEPTIDE LIBRARY;

AMINO ACID SUBSTITUTION; AMINO TERMINAL SEQUENCE; ARTICLE; BRIGHTNESS; CARBOXY TERMINAL SEQUENCE; CHEMICAL PARAMETERS; CLINICAL PROTOCOL; CRYSTAL STRUCTURE; DIMERIZATION; DIRECTED MOLECULAR EVOLUTION; EXPERIMENTAL DESIGN; EXTINCTION COEFFICIENT; FLUORESCENCE ACTIVATED CELL SORTING; FLUORESCENCE MICROSCOPY; GEL FILTRATION; IMAGING AND DISPLAY; INTERMETHOD COMPARISON; LIVE CELL IMAGING; MOLECULAR LIBRARY; MONOMERIZATION; PH; PHOTOSTABILITY; POLYMERIZATION; PRIORITY JOURNAL; PROCEDURES CONCERNING CELLS; PROCESS OPTIMIZATION; PROTEIN ANALYSIS; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN PURIFICATION; QUANTUM YIELD; STRUCTURE ACTIVITY RELATION; CHEMISTRY; GENETICS; PEPTIDE LIBRARY; PROCEDURES;

DIRECTED MOLECULAR EVOLUTION; FLUORESCENT DYES; GREEN FLUORESCENT PROTEINS; PEPTIDE LIBRARY; PROTEIN ENGINEERING;

EID: 84897136274     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2014.054     Document Type: Article

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