Engineering of a fungal β-galactosidase to remove product inhibition by galactose

Applied Microbiology and Biotechnology

Volumn 87, Issue 5, 2010, Pages 1773-1782

  Hu, Xuejun ^a,b,c   Robin, Sylvain ^b   O'Connell, Shane ^b   Walsh, Gary ^b   Wall, J Gerard ^a,b  

^a NATIONAL UNIVERSITY OF IRELAND   (Ireland)

^b UNIVERSITY OF LIMERICK   (Ireland)

^c DALIAN UNIVERSITY   (China)

Author keywords

galactosidase; Aspergillus niger van Tiegh; Galactose; Lactase; Mutagenesis; Product inhibition

Indexed keywords

AMINO ACID MOTIFS; ASPERGILLUS NIGER; ASPERGILLUS NIGER VAN TIEGH; ENZYME STRUCTURES; GALACTOSIDASES; GASTRIC CONDITIONS; KINETIC PROPERTIES; MUTATED ENZYMES; NATIVE ENZYMES; NIGER; PENICILLIUM SP; PICHIA PASTORIS; PRODUCT INHIBITION; RECOMBINANT ENZYMES; RECOMBINANT PROTEIN; REDUCED SENSITIVITY; WILD TYPES; WILD-TYPE ENZYMES;

AMINO ACIDS; CRYSTAL STRUCTURE; ENZYMES; GENE ENCODING; MUTAGENESIS; ORGANIC ACIDS; SUGARS;

ENZYME INHIBITION;

ASPARTIC ACID; BETA GALACTOSIDASE; GALACTOSE; GLYCINE; LACTASE; LEUCINE; PHENYLALANINE; PROLINE; SERINE; TYROSINE; ENZYME INHIBITOR; FUNGAL DNA; FUNGAL PROTEIN; MUTANT PROTEIN; RECOMBINANT PROTEIN;

AMINO ACID; BIOENGINEERING; CRYSTAL STRUCTURE; ENZYME ACTIVITY; FUNGUS; GENE EXPRESSION; INHIBITION; MONOSACCHARIDE; MUTATION; PROTEIN; RECOMBINATION; TOLERANCE;

ARTICLE; ASPERGILLUS NIGER; CRYSTAL STRUCTURE; ENZYME ENGINEERING; ENZYME INHIBITION; ENZYME ISOLATION; ENZYME KINETICS; ENZYME STABILITY; ENZYME STRUCTURE; LACTOSE TOLERANCE; MUTAGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN MOTIF; CHEMICAL STRUCTURE; CHEMISTRY; DNA SEQUENCE; ENZYMOLOGY; GENE EXPRESSION; GENETICS; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; PICHIA; PROTEIN ENGINEERING; PROTEIN TERTIARY STRUCTURE; SITE DIRECTED MUTAGENESIS;

ASPERGILLUS NIGER; BETA-GALACTOSIDASE; CLONING, MOLECULAR; DNA, FUNGAL; ENZYME INHIBITORS; FUNGAL PROTEINS; GALACTOSE; GENE EXPRESSION; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTANT PROTEINS; PICHIA; PROTEIN ENGINEERING; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA;

ASPERGILLUS NIGER; PENICILLIUM SP.; PICHIA PASTORIS;

EID: 77955550139     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-010-2662-8     Document Type: Article

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