Massively parallel delivery of large cargo into mammalian cells with light pulses

Nature Methods

Volumn 12, Issue 5, 2015, Pages 439-444

  Wu, Yi Chien ^a   Wu, Ting Hsiang ^a,b   Clemens, Daniel L ^a   Lee, Bai Yu ^a   Wen, Ximiao ^a   Horwitz, Marcus A ^a   Teitell, Michael A ^a,b   Chiou, Pei Yu ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; ELEMENT; ENZYME; NANOPARTICLE;

ANALYTICAL EQUIPMENT; ARTICLE; BACTERIAL GENE; BIOPHOTONIC LASER ASSISTED SURGERY TOOL; CELL DIVISION; CELL MEMBRANE; CELL VIABILITY; CONTROLLED STUDY; CYTOSOL; ENDOCYTOSIS; FEMALE; FLOW; FRANCISELLA NOVICIDA; HUMAN; HUMAN CELL; LIGHT; MAMMAL CELL; NONHUMAN; PATHOGENESIS; PHAGOSOME; PRESSURE; PRIORITY JOURNAL; TIME; ANIMAL; CELL LINE; FRANCISELLA; GENE EXPRESSION REGULATION; LASER; MICROBUBBLE; PHYSIOLOGY;

FRANCISELLA TULARENSIS SUBSP. NOVICIDA; MAMMALIA;

ANIMALS; CELL LINE; FRANCISELLA; GENE EXPRESSION REGULATION, BACTERIAL; HUMANS; LASERS; MICROBUBBLES;

EID: 84928928784     PISSN: 15487091     EISSN: 15487105     Source Type: Journal    
DOI: 10.1038/nmeth.3357     Document Type: Article

References (34)

1. Rusk, N. Seamless delivery. Nat. Methods 8, 44 (2011).
2. Naldini, L. et al. In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector. Science 272, 263-267 (1996).
3. Akin, D. et al. Bacteria-mediated delivery of nanoparticles and cargo into cells. Nat. Nanotechnol. 2, 441-449 (2007).
4. Felgner, P.L. et al. Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure. Proc. Natl. Acad. Sci. USA 84, 7413-7417 (1987).
5. De Smedt, S.C., Demeester, J. & Hennink, W.E. Cationic polymer based gene delivery systems. Pharm. Res. 17, 113-126 (2000).
6. Somiari, S. et al. Theory and in vivo application of electroporative gene delivery. Mol. Ther. 2, 178-187 (2000).
7. Guignet, E.G. & Meyer, T. Suspended-drop electroporation for high-throughput delivery of biomolecules into cells. Nat. Methods 5, 393-395 (2008).
8. Boukany, P.E. et al. Nanochannel electroporation delivers precise amounts of biomolecules into living cells. Nat. Nanotechnol. 6, 747-754 (2011).
9. Kim, H.J., Greenleaf, J.F., Kinnick, R.R., Bronk, J.T. & Bolander, M.E. Ultrasound-mediated transfection of mammalian cells. Hum. Gene Ther. 7, 1339-1346 (1996).
10. Mitragotri, S. Healing sound: the use of ultrasound in drug delivery and other therapeutic applications. Nat. Rev. Drug Discov. 4, 255-260 (2005).
11. Tirlapur, U.K. & König, K. Cell biology: targeted transfection by femtosecond laser. Nature 418, 290-291 (2002).
12. Tao, W., Wilkinson, J., Stanbridge, E.J. & Berns, M.W. Direct gene transfer into human cultured cells facilitated by laser micropuncture of the cell membrane. Proc. Natl. Acad. Sci. USA 84, 4180-4184 (1987).
13. Chakravarty, P., Qian, W., El-Sayed, M.A. & Prausnitz, M.R. Delivery of molecules into cells using carbon nanoparticles activated by femtos laser pulses. Nat. Nanotechnol. 5, 607-611 (2010).
14. Sharei, A. et al. A vector-free microfluidic platform for intracellular delivery. Proc. Natl. Acad. Sci. USA 110, 2082-2087 (2013).
15. Shalek, A.K. et al. Vertical silicon nanowires as a universal platform for delivering biomolecules into living cells. Proc. Natl. Acad. Sci. USA 107, 1870-1875 (2010).
16. Capecchi, M.R. High efficiency transformation by direct microinjection of DNA into cultured mammalian cells. Cell 22, 479-488 (1980).
17. Zhang, Y. & Yu, L.-C. Microinjection as a tool of mechanical delivery. Curr. Opin. Biotechnol. 19, 506-510 (2008).
18. Hurtig, J. & Orwar, O. Injection and transport of bacteria in nanotube-vesicle networks. Soft Matter 4, 1515-1520 (2008).
19. Wu, T.-H. et al. Photothermal nanoblade for large cargo delivery into mammalian cells. Anal. Chem. 83, 1321-1327 (2011).
20. Hartland, G.V. Optical studies of dynamics in noble metal nanostructures. Chem. Rev. 111, 3858-3887 (2011).
21. Link, S. & El-Sayed, M.A. Spectral properties and relaxation dynamics of surface plasmon electronic oscillations in gold and silver nanodots and nanorods. J. Phys. Chem. B 103, 8410-8426 (1999).
22. Kotaidis, V., Dahmen, C., von Plessen, G., Springer, F. & Plech, A. Excitation of nanoscale vapor bubbles at the surface of gold nanoparticles in water. J. Chem. Phys. 124, 184702 (2006).
23. Lukianova-Hleb, E. et al. Plasmonic nanobubbles as transient vapor nanobubbles generated around plasmonic nanoparticles. ACS Nano 4, 2109-2123 (2010).
24. Furlani, E.P., Karampelas, I.H. & Xie, Q. Analysis of pulsed laser plasmon-assisted photothermal heating and bubble generation at the nanoscale. Lab Chip 12, 3707-3719 (2012).
25. Yamane, D. et al. Electrical impedance monitoring of photothermal porated mammalian cells. J. Lab. Autom. 19, 50-59 (2014).
26. Marquis, H., Doshi, V. & Portnoy, D.A. The broad-range phospholipase C and a metalloprotease mediate listeriolysin O-independent escape of Listeria monocytogenes from a primary vacuole in human epithelial cells. Infect. Immun. 63, 4531-4534 (1995).
27. Clemens, D.L., Lee, B.-Y. & Horwitz, M.A. Virulent and avirulent strains of Francisella tularensis prevent acidification and maturation of their phagosomes and escape into the cytoplasm in human macrophages. Infect. Immun. 72, 3204-3217 (2004).
28. Nano, F.E. & Schmerk, C. The Francisella pathogenicity island. Ann. NY Acad. Sci. 1105, 122-137 (2007).
29. Barker, J.R. et al. The Francisella tularensis pathogenicity island encodes a secretion system that is required for phagosome escape and virulence. Mol. Microbiol. 74, 1459-1470 (2009).
30. Nano, F.E. et al. A Francisella tularensis pathogenicity island required for intramacrophage growth. J. Bacteriol. 186, 6430-6436 (2004).
31. de Bruin, O.M. et al. The biochemical properties of the Francisella pathogenicity island (FPI)-encoded proteins IglA, IglB, IglC, PdpB and DotU suggest roles in type VI secretion. Microbiology 157, 3483-3491 (2011).
32. Checroun, C., Wehrly, T.D., Fischer, E.R., Hayes, S.F. & Celli, J. Autophagy-mediated reentry of Francisella tularensis into the endocytic compartment after cytoplasmic replication. Proc. Natl. Acad. Sci. USA 103, 14578-14583 (2006).
33. Golovliov, I., Sjöstedt, A., Mokrievich, A. & Pavlov, V. A method for allelic replacement in Francisella tularensis. FEMS Microbiol. Lett. 222, 273-280 (2003).
34. Jones, J.W. et al. Absent in melanoma 2 is required for innate immune recognition of Francisella tularensis. Proc. Natl. Acad. Sci. USA 107, 9771-9776 (2010).