Enhanced production of leech hyaluronidase by optimizing secretion and cultivation in Pichia pastoris

Applied Microbiology and Biotechnology

Volumn 100, Issue 2, 2016, Pages 707-717

  Kang, Zhen ^a,b   Zhang, Na ^a   Zhang, Yunfeng ^a  

^a JIANGNAN UNIVERSITY   (China)

^b Synergetic Innovation Center of Food Safety and Nutrition   (China)

Author keywords

Amphipathic peptides; Leech hyaluronidase; N terminal engineering; Pichia pastoris; Signal peptides; Variable temperature control strategy

Indexed keywords

CLONING; PEPTIDES;

AMPHIPATHIC PEPTIDES; CONTROL STRATEGIES; LEECH HYALURONIDASE; N-TERMINALS; PICHIA PASTORIS; SIGNAL PEPTIDE;

YEAST;

ALCOHOL OXIDASE; HYALURONIDASE; LEECH HYALURONIDASE; LIPASE B; RECOMBINANT ENZYME; SIGNAL PEPTIDE; UNCLASSIFIED DRUG; HYALURONOGLUCOSAMINIDASE;

BIOTECHNOLOGY; ENZYME ACTIVITY; FERMENTATION; LEECH; OPTIMIZATION; PEPTIDE; SECRETION; YEAST;

AMINO TERMINAL SEQUENCE; ARTICLE; CANDIDA ANTARCTICA; CELL VIABILITY; ENZYME RELEASE; ENZYME SYNTHESIS; ESCHERICHIA COLI; FED BATCH FERMENTATION; FERMENTATION; GENE DOSAGE; GENETIC SCREENING; GENETIC TRANSFORMATION; KOMAGATAELLA PASTORIS; NONHUMAN; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION; THERMOSTABILITY; ANIMAL; BIOSYNTHESIS; CHEMISTRY; ENZYME STABILITY; ENZYMOLOGY; GENETIC ENGINEERING; GENETICS; KINETICS; LEECH; MOLECULAR CLONING; PH; PHYSIOLOGY; PICHIA; TEMPERATURE;

HIRUDINIDA; PICHIA PASTORIS;

ANIMALS; CLONING, MOLECULAR; ENZYME STABILITY; GENETIC ENGINEERING; HYALURONOGLUCOSAMINIDASE; HYDROGEN-ION CONCENTRATION; KINETICS; LEECHES; PICHIA; PROTEIN SORTING SIGNALS; TEMPERATURE;

EID: 84953634931     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-015-7056-5     Document Type: Article

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