High-level intracellular expression of hydroxynitrile lyase from the tropical rubber tree Hevea brasiliensis in microbial hosts

Protein Expression and Purification

Volumn 11, Issue 1, 1997, Pages 61-71

  Hasslacher, Meinhard ^a   Schall, Michael ^b   Hayn, Marianne ^b   Bona, Rudolfo ^a   Rumbold, Karl ^a   Lückl, Johannes ^a   Griengl, Herfried ^a   Kohlwein, Sepp D ^a   Schwab, Helmut ^a  

^a GRAZ UNIVERSITY OF TECHNOLOGY   (Austria)

^b UNIVERSITY OF GRAZ   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

HEVEA BRASILIENSIS;

EID: 0031259717     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1997.0765     Document Type: Article

References (41)

1. 1. Kruse, C. G. (1992) Chiral cyanohydrins - Their manufacture and utility as chiral building blocks, in "Chirality in Industry" (Collins, A. N., Sheldrake, G. N., and Crosby, J., Eds.), pp. 279-299, Wiley, New York.
2. 2. van der Werf, M. J., van der Will, W. J. J., Kamphuis, J., Hartmans, S., and de Bont, J. A. M. (1994) The potential of lyases for the industrial production of optically active compounds. Trends Biotechnol. 12, 95-103.
3. 3. Gerstner, E., and Pfeil, E. (1972) Zur kenntnis des flavinenzyms hydroxynitril-lyase (D-oxynitrilase). Hoppe-Seyler's Z. Physiol. Chem. 353, 271-286.
4. 4. Gerstner, E., and Kiel, U. (1975) Eine neue mandelsäurenitrillyase (D-oxynitrilase) aus Prunus laurocerasus (Kirschlorbeer). Hoppe-Seyler's Z. Physiol. Chem. 356, 1853-1857.
5. 5. Kiempier, N., Griengl, H., and Hayn, M. (1993) Aliphatic (S)-cyanohydrins by enzyme-catalyzed synthesis. Tetrahedron Lett. 34, 4769-4772.
6. 6. Kiempier, N., Pichier, U., and Griengl, H. (1995) Synthesis of α/ β-unsaturated (S)-cyanohydrins using oxynitrilase from Hevea brasiliensis. Tetrahedron: Asymmetry 6, 845-848.
7. 7. Schall, M. (1996) "Isolation and Characterization of a (S)-Hydroxynitrile Lyase from Hevea brasiliensis," PhD thesis, University of Graz, Austria.
8. 8. Hasslacher, M., Schall, M., Hayn, M., Griengl, H., Kohlwein, S. D., and Schwab, H. (1996) Molecular cloning of the full-length cDNA of (S)-hydroxynitrile lyase from Hevea brasiliensis. J. Biol. Chem. 271, 5884-5891.
9. 9. Hasslacher, M., Kratky, C., Griengl, H., Schwab, H., and Kohlwein, S. D. (1997) Hydroxynitrile lyase from Hevea brasiliensis: Molecular characterization and mechanism of enzyme catalysis. Proteins 27, 438-449.
10. 10. Wagner, U. G., Hasslacher, M., Griengl, H., Schwab, H., and Kratky, C. (1996) Mechanism of cyanogenesis: The crystal structure of hydroxynitrile lyase from Hevea brasiliensis. Structure 4, 811-822.
11. 11. Hughes, J., Decarvalho, J., and Hughes, M. A. (1994) Purification, characterization, and cloning of α-hydroxynitrile lyase from cassava (Manihot esculenta crantz). Arch. Biochem. Biophys. 311, 496-502.
12. 12. Wajant, H., Mundry, K. W., and Pfizenmaier, K. (1994) Molecular-cloning of hydroxynitrile lyase from Sorghum bicolor (L) -Homologies to serine carboxypeptidases. Plant Mol. Biol. 26, 735-746.
13. 13. Schoner, B. E., Belagaje, R. M., and Schoner, R. (1990) Enhanced translational efficiency with two-cistron expression systems. Meth. Enzymol. 185, 94-103.
14. 14. Hill, J. E., Myers, A. M., Koerner, T. J., and Tzagloff, A. (1986) Yeast/E. coli shuttle vector with multiple unique restriction sites. Yeast 2, 163-167.
15. 15. Kingsman, S. M., Cousens, D., Stanway, C. A., Chambers, A., Wilson, M., and Kingsman, A. J. (1990) High-efficiency yeast expression vectors based on the promoter of the phosphoglycerate kinase gene. Meth. Enzymol. 185, 329-341.
16. 16. Cregg, J. M., Barringer, K. J., Hessler, A. Y., and Maden, K. R. (1985) Pichia pastoris as host system for transformation. Mol. Cell. Biol. 5, 3376-3385.
17. 17. Ausubel, F., Brent, R., Kingston, R., Moore, D., Seidman, J., Smith, J., and Struhl, K. (Eds.) (1996) "Current Protocols in Molecular Biology," Greene Publishing Associates and Wiley-Interscience, New York.
18. 18. Sanger, F., Niklen, S., and Coulsen, A. R. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74, 5463-5467.
19. 19. Ito, H., Fukuda, Y., Murata, K., and Kimura, A. (1983) Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 153, 163-168.
20. 20. King, J., and Laemmli, U. K. (1971) J. Mol. Biol. 62, 465-477.
21. 21. Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951) Protein measurement with folin phenol reagent. J. Biol. Chem. 193, 265-275.
22. 22. Martson, F. A. O. (1987) The purification of eucaryontic polypeptides expressed in E. coli, in "DNA Cloning: A Practical Approach" (Glover, D. M., Ed.), pp. 59-88, IRL Press, Oxford.
23. 23. Kohno, T., Charmichael, D. F., Sommer, A., and Thompson, R. (1990) Refolding of recombinant proteins. Meth. Enzymol. 185, 187-195.
24. 24. Pringle, J. R., Preston, R. A., Adams, A. E. M., Stearns, T., Drubin, D., Haarer, B. K., and Jones, E. W. (1989) Fluorescence microscopy methods for yeast. Meth. Cell Biol. 31, 357-435.
25. 25. Amann, E., Brosius, J., and Ptashne, M. (1983) Vectors bearing a hybrid trp-lac promoter seful for regulated expression of cloned genes in Escherichia coli. Gene 25, 167-178.
26. 26. Hockney, R. C. (1994) Recent developments in heterologous protein production in Escherichia coli. Trends Biotechnol. 12, 456-463.
27. 27. Schein, C. H., and Noteborn, M. H. M. (1988) Formation of recombinant protein in E. coli is favored by lower growth temperature. Bio/Technology 6, 291-294.
28. 28. Sugimoto, S., Yokoo, Y., Hatakeyama, N., Yotsuji, A., Teshiba, S., and Hagino, H. (1991) Higher culture pH is preferable for inclusion body formation of recombinant salmon growth-hormone in Escherichia coli. Biotechnol. Lett. 13, 385-388.
29. 29. Moore, J. T., Uppal, A., Maley, F., and Maley, G. F. (1993) Overcoming inclusion body formation in a high-level expression system. Protein Expression Purif. 4, 160-163.
30. 30. Goeddel, D. V. (Ed.) (1990) Expression in yeast in "Methods in Enzymology," pp. 231-484, Academic Press, San Diego.
31. 31. Romanos, M. A., Scorer, C. A., and Clare, J. J. (1992) Foreign gene expression in yeast. Yeast 8, 423-488.
32. 32. Kuroda, S., Otaka, S., Miyazaki, T., Nakoa, M., and Fujisawa, Y. (1992) Hepatitis B virus envelope L protein particles. J. Biol. Chem. 267, 1953-1961.
33. 33. Kingsman, S. M., and Kingsman, A. J. (1983) The production of interferon in bacteria and yeast, in "Interferons, Society for General Microbiology Symp." (Birke, D. C., and Morris, A., Ed.), pp. 211-254, Cambridge University Press, Cambridge, UK.
34. 34. Siegel, R. S., Buckholz, R. G., Thill, G. P., and Wondrack, L. M. (1990) "Production of Epidermal Growth Factor in Methylotrophic Yeast Cells," International patent application, Publication WO 90/10697.
35. 35. Brierley, R. A., Bussineau, C., Kosson, R., Melton, A., and Siegel, R. S. (1990) Fermentation development of recombinant Pichia pastoris expressing the heterologous gene: Bovine lysozyme. Ann. NY Acad. Sci. 589, 350-362.
36. 36. Siegel, R. S., and Brierley, R. A. (1989) Methylotrophic yeast Pichia pastoris produced in high-cell-density fermentations with high cell yields as vehicle for recombinant protein production. Biotechnol. Bioeng. 34, 403-404.
37. 37. Cregg, J. M., et al. (1987) High-level expression and efficient assembly of hepatitis B surface antigen in the methylotrophic yeast Pichia pastoris. Bio/Technology 5, 479-485.
38. 38. Sreekrishna, K., et al. (1989) High level expression, purification and characterization of human tumor necrosis factor synthesized in the methylotrophic yeast Pichia pastoris. J. Basic Microbiol. 28, 265-278.
39. 39. Clare, J. J., Rayment, F. B., Ballantine, S. P., Sreekrishna, K., and Romanos, M. A. (1991) High-level expression of tetanus toxin fragment C in Pichia pastoris strains containing multiple tandem integrations of the gene. Bio/Technology 9, 455-460.
40. 40. Kendall, R. L., Yamada, R., and Bradshaw, R. A. (1990) Cotranslational amino-terminal processing. Meth. Enzymol. 185, 398-407.
41. 41. Cregg, J. M., Vedvick, T. S., and Rascke, W. C. (1993) Recent advances in expression of foreign genes in Pichia pastoris. Biol Technology 11, 905-910.