Improved secretion of Candida antarctica lipase B with its native signal peptide in Pichia pastoris

Enzyme and Microbial Technology

Volumn 52, Issue 3, 2013, Pages 177-183

  Vadhana, Ashok Kumar Prasanna ^a   Samuel, Premsingh ^a   Berin, Ronald M ^a   Krishna, Jayachandran ^a   Kamatchi, Kavitha ^a   Meenakshisundaram, Sankaranarayanan ^a  

^a ANNA UNIVERSITY   (India)

Author keywords

Alpha signal peptide; CalB signal peptide; HGMCSF; Lipases; Pichia pastoris

Indexed keywords

ALPHA SIGNALS; ANTARCTICA; CANDIDA ANTARCTICA LIPASE B; COMPARATIVE ANALYSIS; COPY NUMBER; HETEROLOGOUS PROTEINS; HGMCSF; LIPASE A; MATURE PROTEINS; N TERMINUS; N-TERMINAL EXTENSION; PICHIA PASTORIS; PROTEIN EXPRESSIONS; REAL-TIME PCR; REPORTER PROTEIN; SACCHROMYCES CEREVISIAE; SECRETION EFFICIENCY; SIGNAL PEPTIDE; SIGNAL SEQUENCE; TRANSCRIPTIONAL LEVELS;

AMINO ACIDS; CLONING; ISOMERS; PHYSIOLOGY; POLYMERASE CHAIN REACTION; PROTEINS; TRANSCRIPTION; YEAST;

LIPASES;

ACID LIPASE; LIPASE B; RECOMBINANT ENZYME; SIGNAL PEPTIDE;

ARTICLE; CANDIDA ANTARCTICA; COMPARATIVE STUDY; CONTROLLED STUDY; COPY NUMBER VARIATION; ENZYME ACTIVITY; ENZYME RELEASE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PICHIA PASTORIS; PROTEIN ANALYSIS; PROTEIN PROCESSING; REAL TIME POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION;

AMINO ACID SEQUENCE; BASE SEQUENCE; BIOREACTORS; CANDIDA; CLONING, MOLECULAR; ENZYME PRECURSORS; FUNGAL PROTEINS; GENE DOSAGE; GENES, REPORTER; GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR; HUMANS; INDUSTRIAL MICROBIOLOGY; LIPASE; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; PICHIA; PROTEIN SORTING SIGNALS; REAL-TIME POLYMERASE CHAIN REACTION; RECOMBINANT FUSION PROTEINS; RNA, FUNGAL; RNA, MESSENGER; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; TRANSCRIPTION INITIATION, GENETIC;

CANDIDA ANTARCTICA; PICHIA PASTORIS;

EID: 84873715274     PISSN: 01410229     EISSN: 18790909     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2013.01.001     Document Type: Article

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