Application of simple fed-batch technique to high-level secretory production of insulin precursor using Pichia pastoris with subsequent purification and conversion to human insulin

Microbial Cell Factories

Volumn 9, Issue , 2010, Pages

  Gurramkonda, Chandrasekhar ^a,b   Polez, Sulena ^b   Skoko, Natasa ^b   Adnan, Ahmad ^a,c   Gäbel, Thomas ^a,c   Chugh, Dipti ^b   Swaminathan, Sathyamangalam ^b   Khanna, Navin ^b   Tisminetzky, Sergio ^b   Rinas, Ursula ^a  

^a HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

^b INTERNATIONAL CENTRE FOR GENETIC ENGINEERING AND BIOTECHNOLOGY   (Italy)

^c FRAUNHOFER INSTITUTE FOR TOXICOLOGY AND EXPERIMENTAL MEDICINE   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

GLYCEROL; METHANOL; PREPROINSULIN; RECOMBINANT HUMAN INSULIN; TRYPSIN; INSULIN;

ARTICLE; BIOTRANSFORMATION; BROTH DILUTION; CELL DENSITY; CELL FREE SYSTEM; CULTURE MEDIUM; DEPROTECTION REACTION; DRUG PURIFICATION; DRUG SYNTHESIS; FUNGAL STRAIN; FUNGUS CULTURE; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; INSULIN RELEASE; INSULIN SYNTHESIS; MOLECULAR CLONING; NONHUMAN; PICHIA PASTORIS; PROTEIN EXPRESSION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; TRANSESTERIFICATION; BIOSYNTHESIS; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; PHARMACEUTICS; PICHIA; SECRETION;

PICHIA; PICHIA PASTORIS; SACCHAROMYCES CEREVISIAE;

CLONING, MOLECULAR; CULTURE MEDIA; GLYCEROL; HUMANS; INSULIN; METHANOL; PICHIA; TECHNOLOGY, PHARMACEUTICAL;

EID: 77951999991     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/1475-2859-9-31     Document Type: Article

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