Expression of a cold-adapted fish trypsin in Pichia pastoris

FEMS Yeast Research

Volumn 5, Issue 9, 2005, Pages 851-857

  Macouzet, Martin ^a   Simpson, Benjamin K ^a   Lee, Byong H ^a,b  

^a MCGILL UNIVERSITY   (Canada)

^b FOOD RESEARCH AND DEVELOPMENT CENTRE   (Canada)

Author keywords

Cold adapted trypsin; Cunner fish; Pichia pastoris; Tautogolabrus adspersus; Trypsinogen

Indexed keywords

COLD ADAPTED FISH TRYPSIN; ENTEROPEPTIDASE; NICKEL; RECOMBINANT PROTEIN; SIGNAL PEPTIDE; TRYPSIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; BIOTECHNOLOGY; COLD ACCLIMATIZATION; CONTROLLED STUDY; ENZYME ACTIVATION; EXPRESSION VECTOR; FISH; FOOD PROCESSING; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PICHIA PASTORIS; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; PROTEIN TARGETING; SIGNAL TRANSDUCTION; WESTERN BLOTTING;

ANIMALS; BLOTTING, WESTERN; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; DNA; ENTEROPEPTIDASE; GENETIC VECTORS; PERCIFORMES; PICHIA; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; TEMPERATURE; TRANSFORMATION, GENETIC; TRYPSIN; VISCOSITY;

ANIMALIA; BOVINAE; PICHIA; PICHIA PASTORIS; TAUTOGOLABRUS ADSPERSUS;

EID: 19544389696     PISSN: 15671356     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.femsyr.2005.02.007     Document Type: Article

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