Antibody fragments may be incorrectly processed in the yeast Pichia pastoris

Biotechnology Letters

Volumn 30, Issue 9, 2008, Pages 1661-1663

  Kozlov, D G ^a   Yagudin, T A ^a  

^a STATE RESEARCH INSTITUTE OF GENETICS AND SELECTION OF INDUSTRIAL MICROORGANISMS   (Russian Federation)

Author keywords

Antibody light chain; Leader sequence; Pichia pastoris; Pro protein processing

Indexed keywords

PORT TERMINALS;

ANTIBODY FRAGMENTS; ANTIBODY LIGHT CHAIN; LEADER SEQUENCE; LIGHT CHAIN; N TERMINALS; PICHIA PASTORIS; PRO-PROTEIN PROCESSING; SACCHAROMYCES CEREVISIAE;

YEAST;

IMMUNOGLOBULIN FRAGMENT; PROTEIN PRECURSOR;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; CHEMISTRY; HUMAN; METABOLISM; MOLECULAR GENETICS; MOUSE; PICHIA; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN PROCESSING;

AMINO ACID SEQUENCE; ANIMALS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; IMMUNOGLOBULIN FRAGMENTS; MICE; MOLECULAR SEQUENCE DATA; PICHIA; PROTEIN PRECURSORS; PROTEIN PROCESSING, POST-TRANSLATIONAL;

MURINAE; PICHIA PASTORIS; SACCHAROMYCES CEREVISIAE;

EID: 49749108920     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-008-9739-9     Document Type: Article

References (12)

1. DC Andersen DE Reilly 2004 Production technologies for monoclonal antibodies and their fragments Curr Opin Biotechnol 15 456 462
2. A Bevan C Brenner RS Fuller 1998 Quantitative assessment of enzyme specificity in vivo: P2 recognition by Kex2 protease defined in a genetic system Proc Nat Acad Sci USA. 95 10384 10389
3. JL Cereghino JM Cregg 2000 Heterologous protein expression in the methylotrophic yeast Pichia pastoris FEMS Microbiol Rev 24 45 66
4. R Daly TW Hearn 2005 Expression of heterologous proteins in Pichia pastoris: a useful experimental tool in protein engineering and production J Mol Recognit 18 119 138
5. J Folch M Lees GH Sloane Stanley 1957 A simple method for isolation and purification of total lipids from animal tissues J Biol Chem 226 497 509
6. P Holliger 2001 Expression of antibody fragments in Pichia pastoris Methods Mol Biol 178 349 357
7. EC Ledgerwood PM George RJ Peach SO Brennan 1995 Endoproteolytic processing of recombinant proalbumin variants by the yeast Kex2 protease Biochem J 308 321 325
8. V Olsen NX Cawley J Brandt M Egel-Mitani YP Loh 1999 Identification and characterization of Saccharomyces cerevisiae. yapsin 3, a new member of the yapsin family of aspartic proteases encoded by the YPS3 gene Biochem J 339 407 411
9. M Rholam N Brakch D Germain DY Thomas C Fahy H Boussetta G Boileau P Cohen 1995 Role of amino acid sequences flanking dibasic cleavage sites in precursor proteolytic processing. The importance of the first residue C-terminal of the cleavage site Eur J Biochem 227 707 714
10. NC Rockwell RS Fuller 1998 Interplay between S1 and S4 subsites in Kex2 protease: Kex2 exhibits dual specificity for the P4 side chain Biochemistry 37 3386 3391
11. Y Suzuki N Ikeda E Kataoka K Ohsuye 2000 Effect of amino acid substitution on the P(3) and P(4) subsites of fusion proteins on kex2 protease activity J Biotechnol Appl Biochem 32 53 60
12. MWT Werten FA de Wolf 2005 Reduced proteolysis of secreted gelatin and Yps1-mediated α-factor leader processing in a Pichia pastoris kex2 disruptant Appl Environ Microbiol 71 2310 2317