High-yield secretion of recombinant gelatins by Pichia pastoris

Yeast

Volumn 15, Issue 11, 1999, Pages 1087-1096

  Werten, Marc W T ^a   Van Den Bosch, Tanja J ^a   Wind, Richèle D ^a   Mooibroek, Hans ^a   De Wolf, Frits A ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

Author keywords

Collagen; Expression; Gelatin; Kex2; Pichia pastoris; Proteolysis; Secretion

Indexed keywords

CASAMINO ACID; COLLAGEN TYPE 1; COLLAGEN TYPE 3; GELATIN; PROTEINASE; RECOMBINANT PROTEIN;

ARTICLE; CONTROLLED STUDY; FERMENTATION; NONHUMAN; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN SECRETION; SACCHAROMYCES CEREVISIAE; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; COLLAGEN; DNA; DNA PRIMERS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FERMENTATION; GELATIN; GENETIC VECTORS; HYDROGEN-ION CONCENTRATION; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PICHIA; PLASMIDS; PROPROTEIN CONVERTASES; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SEQUENCE ANALYSIS; SUBTILISINS; TRANSFORMATION, GENETIC;

PICHIA PASTORIS; SACCHAROMYCES CEREVISIAE;

EID: 0032790856     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0061(199908)15:11<1087::AID-YEA436>3.0.CO;2-F     Document Type: Article

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