High-level extracellular production of alkaline polygalacturonate lyase in Bacillus subtilis with optimized regulatory elements

Bioresource Technology

Volumn 146, Issue , 2013, Pages 543-548

  Zhang, Junjiao ^a   Kang, Zhen ^a   Ling, Zhenmin ^a   Cao, Wenlong ^a   Liu, Long ^a   Wang, Miao ^a   Du, Guocheng ^a   Chen, Jian ^a  

^a JIANGNAN UNIVERSITY   (China)

Author keywords

Alkaline polygalacturonate lyase; Bacillus subtilis; Bpr; Promoter P43; Signal peptide

Indexed keywords

OPTIMIZATION;

BACILLUS SUBTILIS; BPR; POLYGALACTURONATES; PROMOTER P43; SIGNAL PEPTIDE;

GENE ENCODING;

PECTATE LYASE; SIGNAL PEPTIDE;

BACTERIOLOGY; BACTERIUM; BIOTECHNOLOGY; PEPTIDE; RECOMBINATION; SECRETION;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL STRAIN; BACTERIUM CULTURE; CELL GROWTH; CONTROLLED STUDY; DNA SEQUENCE; ENZYME ACTIVITY; ENZYME RELEASE; ENZYME SYNTHESIS; METABOLIC ENGINEERING; NONHUMAN; OPTICAL DENSITY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; SITE DIRECTED MUTAGENESIS; SPECTROPHOTOMETRY;

BACILLUS SUBTILIS;

ALKALINE POLYGALACTURONATE LYASE; BACILLUS SUBTILIS; BPR; PROMOTER P43; SIGNAL PEPTIDE;

BACILLUS SUBTILIS; BIOREACTORS; CULTURE MEDIA; ESCHERICHIA COLI; FERMENTATION; HYDROGEN-ION CONCENTRATION; MUTAGENESIS, SITE-DIRECTED; OLIGONUCLEOTIDES; PEPTIDES; PLASMIDS; POLYGALACTURONASE; POLYSACCHARIDE-LYASES; PROMOTER REGIONS, GENETIC; RECOMBINANT PROTEINS; TIME FACTORS;

EID: 84882962610     PISSN: 09608524     EISSN: 18732976     Source Type: Journal    
DOI: 10.1016/j.biortech.2013.07.129     Document Type: Article

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