Improving Performance and Operational Stability of Porcine Interferon-α Production by Pichia pastoris with Combinational Induction Strategy of Low Temperature and Methanol/Sorbitol Co-feeding

Applied Biochemistry and Biotechnology

Volumn 176, Issue 2, 2015, Pages 493-504

  Gao, Min Jie ^a   Zhan, Xiao Bei ^a   Gao, Peng ^a   Zhang, Xu ^a   Dong, Shi Juan ^b   Li, Zhen ^b   Shi, Zhong Ping ^a   Lin, Chi Chung ^a  

^a JIANGNAN UNIVERSITY   (China)

^b INSTITUTE OF EDIBLE FUNGI   (China)

Author keywords

Fed batch culture; Fermentation; Pichia pastoris; Protein expression; Sorbitol

Indexed keywords

ALCOHOLS; ANTIBIOTICS; BATCH CELL CULTURE; FEEDING; FERMENTATION; GLYCOPROTEINS; METABOLISM; METHANOL; PHYSIOLOGY; PROTEINS; TEMPERATURE; YEAST;

FED-BATCH CULTURES; FORMALDEHYDE OXIDATION; IMPROVING PERFORMANCE; METHANOL CONCENTRATION; PERFORMANCE ENHANCEMENTS; PICHIA PASTORIS; PROTEIN EXPRESSIONS; SORBITOL;

LOW TEMPERATURE PRODUCTION;

ADENOSINE TRIPHOSPHATE; ALCOHOL OXIDASE; ALPHA INTERFERON; METHANOL; PROTEINASE; SORBITOL; TRICARBOXYLIC ACID; RECOMBINANT PROTEIN;

ALCOHOL METABOLISM; ANTIVIRAL ACTIVITY; ARTICLE; BIOSYNTHESIS; CARBON SOURCE; CELL GROWTH; CITRIC ACID CYCLE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ENERGY METABOLISM; ENZYME ACTIVITY; ENZYME INDUCTION; ENZYME RELEASE; FED BATCH CULTURE; FED BATCH FERMENTATION; GLYCOLYSIS; KOMAGATAELLA PASTORIS; LOW TEMPERATURE PROCEDURES; NONHUMAN; OXIDATION; PROTEIN EXPRESSION; PROTEIN HYDROLYSIS; PROTEIN INDUCTION; PROTEIN STABILITY; ANIMAL; GENE EXPRESSION; GENETICS; GROWTH, DEVELOPMENT AND AGING; PICHIA; PIG;

PICHIA PASTORIS; SUS;

ANIMALS; GENE EXPRESSION; INTERFERON-ALPHA; METHANOL; PICHIA; PROTEIN STABILITY; RECOMBINANT PROTEINS; SORBITOL; SWINE;

EID: 84929842521     PISSN: 02732289     EISSN: 15590291     Source Type: Journal    
DOI: 10.1007/s12010-015-1590-6     Document Type: Article

References (33)

1. Cereghino, J. L., & Cregg, J. M. (2000). Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiology Reviews, 24, 45–66.
2. Macauley-Patrick, S., Fazenda, M. L., McNeil, B., & Harvey, L. M. (2005). Heterologous protein production using the Pichia pastoris expression system. Yeast, 22, 249–270.
3. Chinsangaram, J., Moraes, M. P., Koster, M., & Grubman, M. J. (2003). Novel viral disease control strategy: adenovirus expressing alpha interferon rapidly protects swine from foot-and-mouth disease. Joural of Virolory, 77, 1621–1625.
4. Chang, H. W., Jeng, C. R., Liu, J. J., Lin, T. L., Chang, C. C., Chia, M. Y., Tsai, Y. C., & Pang, V. F. (2005). Reduction of porcine reproductive and respiratory syndrome virus (PRRSV) infection in swine alveolar macrophages by porcine circovirus 2 (PCV2)-induced interferon-alpha. Veterinary Microbiology, 108, 167–177.
5. Cereghino, G. P., Cereghino, J. L., Ilgen, C., & Cregg, J. M. (2002). Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris. Current Opinion in Biotechnology, 13, 329–332.
6. Ohya, T., Ohyama, M., & Kobayashi, K. (2006). Optimization of human serum albumin production in methylotrophic yeast Pichia pastoris by repeated fed-batch fermentation. Biotechnology and Bioengineering, 90, 876–887.
7. Khatri, N. K., & Hoffmann, F. (2006). Impact of methanol concentration on secreted protein production in oxygen-limited cultures of recombinant Pichia pastoris. Biotechnology and Bioengineering, 93, 871–879.
8. Gao, M. J., Zhan, X. B., Zheng, Z. Y., Wu, J. R., Dong, S. J., Li, Z., Shi, Z. P., & Lin, C. C. (2013). Enhancing pIFN-α production and process stability in fed-batch culture of Pichia pastoris by controlling the methanol concentration and monitoring the responses of OUR/DO levels. Applied Biochemistry and Biotechnology, 171, 1262–1275.
9. Yu, R. S., Dong, S. J., Zhu, Y. M., Jin, H., Gao, M. J., Duan, Z. Y., Zheng, Z. Y., Shi, Z. P., & Li, Z. (2010). Effective and stable porcine interferon-α production by Pichia pastoris fed-batch cultivation with multi-variables clustering and analysis. Bioprocess and Biosystems Engineering, 33, 473–483.
10. Jahic, M., Wallberg, F., Bollok, M., Garcia, P., & Enfors, S. (2003). Temperature limited fed-batch technique for control of proteolysis in Pichia pastoris bioreactor cultures. Microbial Cell Factories, 2(1), 6.
11. Jin, H., Liu, G. Q., Ye, X. F., Duan, Z. Y., Li, Z., & Shi, Z. P. (2010). Enhanced porcine interferon-α production by recombinant Pichia pastoris with a combinational control strategy of low induction temperature and high dissolved oxygen concentration. Biochemical Engineering Journal, 52, 91–98.
12. Dragosits, M., Stadlmann, J., Albiol, J., Baumann, K., Maurer, M., Gasser, B., Sauer, M., Altmann, F., Ferrer, P., & Mattanovich, D. (2009). The effect of temperature on the proteome of recombinant Pichia pastoris. Journal of Proteome Research, 8, 1380–1392.
13. Wang, Y., Wang, Z. H., Xu, Q. L., Du, G. C., Hua, Z. Z., Liu, L. M., Li, J. H., & Chen, J. (2009). Lowering induction temperature for enhanced production of polygalacturonate lyase in recombinant Pichia pastoris. Process Biochemistry, 44, 949–954.
14. Choi, D. B., & Park, E. Y. (2006). Enhanced production of mouse α-amylase by feeding combined nitrogen and carbon sources in fed-batch culture of recombinant Pichia pastoris. Process Biochemistry, 41, 390–397.
15. Horstkotte, B., Arnau, C., Valero, F., Elsholz, O., & Cerdà, V. (2008). Monitoring of sorbitol in Pichia pastoris cultivation applying sequential injection analysis. Biochemical Engineering Journal, 42, 77–83.
16. Çelik, E., Çalık, P., & Oliver, S. G. (2009). Fed-batch methanol feeding strategy for recombinant protein production by Pichia pastoris in the presence of co-substrate sorbitol. Yeast, 26, 473–484.
17. Jungo, C., Schenk, J., Pasquier, M. M., Marison, I. W., & von Stockar, U. (2007). A quantitative analysis of the benefits of mixed feeds of sorbitol and methanol for production of recombinant avidin with Pichia pastoris. Journal of Biotechnology, 131, 57–66.
18. Ramón, R., Ferrer, P., & Valero, F. (2007). Sorbitol co-feeding reduces metabolic burden caused by the overexpression of a Rhizopus oryzae lipase in Pichia pastoris. Journal Biotechnology, 130, 39–46.
19. Gao, M. J., Li, Z., Yu, R. S., Wu, J. R., Zheng, Z. Y., Shi, Z. P., Zhan, X. B., & Lin, C. C. (2012). Methanol/sorbitol co-feeding induction enhanced porcine interferon-α production by P. pastoris associated with energy metabolism shift. Bioprocess and Biosystems Engineering, 35, 1125–1136.
20. Zhu, T. C., You, L. J., Gong, F. Y., Xie, M. F., Xue, Y. F., Li, Y., & Ma, Y. Y. (2011). Combinatorial strategy of sorbitol feeding and low-temperature induction leads to high-level production of alkaline β-mannanase in Pichia pastoris. Enzyme and Microbial Technology, 49, 407–412.
21. Tian, M. Y., Huitema, E., Da Cunha, L., Torto-Alalibo, T., & Kamoun, S. (2004). A Kazal-like extracellular serine protease inhibitor from Phytophthora infestans targets the tomato pathogenesis-related protease P69B. Journal of Biological Chemistry, 279, 26370–26377.
22. Suye, S., Ogawa, A., Yokoyama, S., & Obayashi, A. (1990). Screening and identification of Candida methanosorbosa as alcohol oxidase-producing methanol using yeast. Agricultural and Biological Chemistry, 54, 1297–1298.
23. Schütte, H., Flossdorf, J., Sahm, H., & Kula, M. R. (1976). Purification and properties of formaldehyde dehydrogenase and formate dehydrogenase from Candida boidinii. European Journal of Biochemistry, 62, 151–160.
24. Gao, M. J., Zheng, Z. Y., Wu, J. R., Dong, S. J., Li, Z., Jin, H., Zhan, X. B., & Lin, C. C. (2012). Improvement of specific growth rate of Pichia pastoris for effective porcine interferon-α production with an on-line model based glycerol feeding strategy. Applied Microbiology and Biotechnology, 93, 1437–1445.
25. + strain in transient continuous cultures. Journal of Biotechnology, 130, 236–246.
26. Trinh, L. B., Phue, J. N., & Shiloach, J. (2003). Effect of methanol feeding strategies on production and yield of recombinant mouse endostatin from Pichia pastoris. Biotechnology and Bioengineering, 82, 438–444.
27. Lim, H. K., Choi, S. J., Kim, K. Y., & Jung, K. H. (2003). Dissolved-oxygen-stat controlling two variables for methanol induction of rGuamerin in Pichia pastoris and its application to repeated fed-batch. Applied Microbiology and Biotechnology, 62, 342–348.
28. Khatri, N. K., & Hoffmann, F. (2006). Oxygen-limited control of methanol uptake for improved production of a single-chain antibody fragment with recombinant Pichia pastoris. Applied Microbiology and Biotechnology, 72, 492–498.
29. Çelik, E., & Çalık, P. (2012). Production of recombinant proteins by yeast cells. Biotechnology Advances, 30, 1108–1118.
30. Lüers, G. H., Advani, R., Wenzel, T., & Subramani, S. (1998). The Pichia pastoris dihydroxyacetone kinase is a PTS1-containing, but cytosolic, protein that is essential for growth on methanol. Yeast, 14, 759–771.
31. Van der Klei, I. J., Yurimoto, H., Sakai, Y., & Veenhuis, M. (2006). The significance of peroxisomes in methanol metabolism in methylotrophic yeast. Biochimica et Biophysica Acta, 12, 1453–1462.
32. Krainer, F. W., Dietzsch, C., Hajek, T., Herwig, C., Spadiut, O., & Glieder, A. (2012). Recombinant protein expression in Pichia pastoris strains with an engineered methanol utilization pathway. Microbial Cell Factories, 11, 22.
33. Schroer, K., Peter, L. K., Hartner, F. S., Glieder, A., & Pscheidt, B. (2010). Engineering the Pichia pastoris methanol oxidation pathway for improved NADH regeneration during whole-cell biotransformation. Metabolic Engineering, 12, 8–17.