Intermediate-sensor assisted push-pull strategy and its application in heterologous deoxyviolacein production in Escherichia coli

Metabolic Engineering

Volumn 33, Issue , 2016, Pages 41-51

  Fang, Mingyue ^a   Wang, Tianmin ^a   Zhang, Chong ^a   Bai, Jili ^a   Zheng, Xiang ^a   Zhao, Xuejin ^b   Lou, Chunbo ^b   Xing, Xin Hui ^a  

^a BEIJING UNIVERSITY OF POSTS AND TELECOMMUNICATIONS   (China)

^b INSTITUTE OF MICROBIOLOGY   (China)

Author keywords

Biosensor; Deoxyviolacein biosynthesis; High throughput screening; Pathway engineering; Sequential optimization

Indexed keywords

BIOCHEMISTRY; BIOSYNTHESIS; ESCHERICHIA COLI; FLOW CYTOMETRY; METABOLITES; THROUGHPUT;

BIOSYNTHETIC PATHWAY; FLUORESCENCE ACTIVATED CELL SORTING; HIGH THROUGHPUT SCREENING; HIGH THROUGHPUT SCREENING TOOLS; PATHWAY ENGINEERINGS; SCREENING METHODS; SECONDARY METABOLITES; SEQUENTIAL OPTIMIZATION;

BIOSENSORS;

AGAR; DEOXYVIOLACEIN; DIPEPTIDE; PIGMENT; UNCLASSIFIED DRUG; ESCHERICHIA COLI PROTEIN; INDOLE DERIVATIVE; TRYPTOPHAN; VIOLACEIN;

ARTICLE; BACTERIAL STRAIN; BIOREACTOR; BIOSENSOR; BIOSYNTHESIS; ESCHERICHIA COLI; FED BATCH FERMENTATION; FLOW CYTOMETRY; FLUORESCENCE ACTIVATED CELL SORTING; GENOTYPE; HIGH THROUGHPUT SCREENING; INTERMEDIATE SENSOR ASSISTED PUSH PULL STRATEGY; LIMIT OF DETECTION; NONHUMAN; PHENOTYPE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; SPECTROFLUOROMETRY; GENETIC ENHANCEMENT; GENETICS; ISOLATION AND PURIFICATION; METABOLIC ENGINEERING; METABOLIC FLUX ANALYSIS; METABOLISM; PHYSIOLOGY; PROCEDURES; SIGNAL TRANSDUCTION;

ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENETIC ENHANCEMENT; INDOLES; METABOLIC ENGINEERING; METABOLIC FLUX ANALYSIS; SIGNAL TRANSDUCTION; TRYPTOPHAN;

EID: 84947734391     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2015.10.006     Document Type: Article

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