The development and application of a single-cell biosensor for the detection of l-methionine and branched-chain amino acids

Metabolic Engineering

Volumn 14, Issue 4, 2012, Pages 449-457

  Mustafi, Nurije ^a   Grünberger, Alexander ^a   Kohlheyer, Dietrich ^a   Bott, Michael ^a   Frunzke, Julia ^a  

^a FORSCHUNGSZENTRUM JÜLICH   (Germany)

Author keywords

Amino acids; Corynebacterium glutamicum; GFP; L valine; Lrp

Indexed keywords

BACTERIAL CELLS; CELL-TO-CELL VARIABILITY; CORYNEBACTERIUM GLUTAMICUM; DIRECTED EVOLUTION; GFP; HIGH-THROUGHPUT; INDUSTRIAL BIOTECHNOLOGY; L-ISOLEUCINE; L-LEUCINE; L-METHIONINE; L-VALINE; LINEAR RELATIONSHIPS; LRP; MICROBIAL STRAIN DEVELOPMENT; ONLINE MONITORING; PYRUVATES; RANDOM MUTAGENESIS; SENSOR DEVICE; SENSOR SYSTEMS; TRANSCRIPTIONAL REGULATOR; WILD-TYPE STRAIN;

BIOSENSORS; CELLS; CYTOLOGY; ENZYME ACTIVITY; SENSORS;

AMINO ACIDS;

ISOLEUCINE; LEUCINE; METHIONINE; PYRUVATE DEHYDROGENASE; VALINE;

AMINO ACID ANALYSIS; ARTICLE; BACTERIAL GROWTH; BACTERIAL STRAIN; BIOACCUMULATION; BIOSENSOR; CONCENTRATION (PARAMETERS); CORYNEBACTERIUM GLUTAMICUM; CYTOPLASM; FLOW CYTOMETRY; FLUORESCENCE ACTIVATED CELL SORTING; FLUORESCENCE ANALYSIS; FLUORESCENCE MICROSCOPY; HIGH THROUGHPUT SCREENING; NONHUMAN; ONLINE MONITORING; OPEN READING FRAME; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN STRUCTURE; SEQUENCE ANALYSIS; TRANSCRIPTION REGULATION; WILD TYPE;

AMINO ACIDS, BRANCHED-CHAIN; BIOSENSING TECHNIQUES; CORYNEBACTERIUM GLUTAMICUM; LEUCINE-RESPONSIVE REGULATORY PROTEIN; METHIONINE; SINGLE-CELL ANALYSIS;

BACTERIA (MICROORGANISMS); CORYNEBACTERIUM GLUTAMICUM;

EID: 84862193202     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2012.02.002     Document Type: Article

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