Enhancing fatty acid production by the expression of the regulatory transcription factor FadR

Metabolic Engineering

Volumn 14, Issue 6, 2012, Pages 653-660

  Zhang, Fuzhong ^a,b,c,d,g   Ouellet, Mario ^a,b   Batth, Tanveer S ^a,b   Adams, Paul D ^a,b   Petzold, Christopher J ^a,b   Mukhopadhyay, Aindrila ^a,b   Keasling, Jay D ^a,b,c,d,e,f  

^a JOINT BIOENERGY INSTITUTE   (United States)

^b LAWRENCE BERKELEY NATIONAL LABORATORY   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d UNIVERSITY OF CALIFORNIA   (United States)

^e UNIVERSITY OF CALIFORNIA   (United States)

^f University of California at Berkeley   (United States)

^g WASHINGTON UNIVERSITY IN ST LOUIS   (United States)

Author keywords

FadR; Fatty acid production; Global regulation; Metabolic engineering

Indexed keywords

ACID PRODUCTION; E. COLI; EXPRESSION LEVELS; FADR; FATTY ACID BIOSYNTHESIS; GLOBAL REGULATION; HIGH YIELD; MEMBRANE TRANSPORT; OPTIMAL LEVEL; OVER-EXPRESSION; PATHWAY GENES; PROTEOMICS; THEORETICAL YIELD; TRANSCRIPTIONAL ANALYSIS; TRANSCRIPTIONAL CHANGES;

BIOCHEMISTRY; ESCHERICHIA COLI; METABOLIC ENGINEERING; MOLECULAR BIOLOGY; TRANSCRIPTION FACTORS;

GENES;

FATTY ACID; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR FADR; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; ESCHERICHIA COLI; FATTY ACID SYNTHESIS; GENE OVEREXPRESSION; GENETIC TRANSCRIPTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; REGULATORY MECHANISM;

3-OXOACYL-(ACYL-CARRIER-PROTEIN) SYNTHASE; ACETYL-COA CARBOXYLASE; ACETYLTRANSFERASES; BACTERIAL PROTEINS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FATTY ACID SYNTHETASE COMPLEX, TYPE II; FATTY ACIDS; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENETIC ENHANCEMENT; REGULATORY ELEMENTS, TRANSCRIPTIONAL; REPRESSOR PROTEINS;

ESCHERICHIA COLI;

EID: 84869039746     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2012.08.009     Document Type: Article

References (31)

1. Alekshun M.N., Levy S.B. The mar regulon: multiple resistance to antibiotics and other toxic chemicals. Trends Microbiol. 1999, 7:410-413.
2. Beller H.R., Goh E.B., Keasling J.D. Genes involved in long-chain alkene biosynthesis in Micrococcus luteus. Appl. Environ. Microbiol. 2010, 76:1212-1223.
3. Brissette J.L., Russel M., Weiner L., Model P. Phage shock protein, a stress protein of Escherichia coli. Proc Natl. Acad. Sci. U S A 1990, 87:862-866.
4. Cronan J.E. In vivo evidence that acyl coenzyme A regulates DNA binding by the Escherichia coli FadR global transcription factor. J. Bacteriol. 1997, 179:1819-1823.
5. Dellomonaco C., Clomburg J.M., Miller E.N., Gonzalez R. Engineered reversal of the beta-oxidation cycle for the synthesis of fuels and chemicals. Nature 2011, 476:355-359.
6. Demple B., Amabile-Cuevas C.F. Redox redux: the control of oxidative stress responses. Cell 1991, 67:837-839.
7. DiRusso C.C. Nucleotide sequence of the fadR gene, a multifunctional regulator of fatty acid metabolism in Escherichia coli. Nucleic Acids Res. 1988, 16:7995-8009.
8. Engl C., Jovanovic G., Lloyd L.J., Murray H., Spitaler M., Ying L., Errington J., Buck M. In vivo localizations of membrane stress controllers PspA and PspG in Escherichia coli. Mol. Microbiol. 2009, 73:382-396.
9. Fujita Y., Matsuoka H., Hirooka K. Regulation of fatty acid metabolism in bacteria. Mol. Microbiol. 2007, 66:829-839.
10. Goh E.B., Baidoo E.E., Keasling J.D., Beller H.R. Engineering of bacterial methyl ketone synthesis for biofuels. Appl. Environ. Microbiol. 2012, 78:70-80.
11. Handke P., Lynch S.A., Gill R.T. Application and engineering of fatty acid biosynthesis in Escherichia coli for advanced fuels and chemicals. Metab. Eng. 2011, 13:28-37.
12. Iram S.H., Cronan J.E. Unexpected functional diversity among FadR fatty acid transcriptional regulatory proteins. J. Biol. Chem. 2005, 280:32148-32156.
13. Knothe G. "Designer" Biodiesel: Optimizing Fatty Ester Composition to Improve Fuel Properties. Energ Fuel 2008, 22:1358-1364.
14. Lee T.S., Krupa R.A., Zhang F., Hajimorad M., Holtz W.J., Prasad N., Lee S.K., Keasling J.D. BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J. Biol. Eng. 2011, 5:12.
15. Lennen R.M., Braden D.J., West R.A., Dumesic J.A., Pfleger B.F. A process for microbial hydrocarbon synthesis: Overproduction of fatty acids in Escherichia coli and catalytic conversion to alkanes. Biotechnol. Bioeng. 2010, 106:193-202.
16. Lennen R.M., Kruziki M.A., Kumar K., Zinkel R.A., Burnum K.E., Lipton M.S., Hoover S.W., Ranatunga D.R., Wittkopp T.M., Marner W.D., Pfleger B.F. Membrane stresses induced by overproduction of free fatty acids in Escherichia coli. Appl. Environ. Microbiol. 2011, 77:8114-8128.
17. Liu T., Khosla C. Genetic engineering of Escherichia coli for biofuel production. Annu. Rev. Genet. 2010, 44:53-69.
18. Liu T., Vora H., Khosla C. Quantitative analysis and engineering of fatty acid biosynthesis in E. coli. Metab. Eng. 2010, 12:378-386.
19. Lutke-Eversloh T., Stephanopoulos G. L-tyrosine production by deregulated strains of Escherichia coli. Appl. Microbiol. Biotechnol. 2007, 75:103-110.
20. MacLean B., Tomazela D.M., Shulman N., Chambers M., Finney G.L., Frewen B., Kern R., Tabb D.L., Liebler D.C., MacCoss M.J. Skyline: an open source document editor for creating and analyzing targeted proteomics experiments. Bioinformatics 2010, 26:966-968.
21. Ouellet M., Adams P.D., Keasling J.D., Mukhopadhyay A. A rapid and inexpensive labeling method for microarray gene expression analysis. BMC Biotechnol. 2009, 9:97.
22. Peralta-Yahya P.P., Ouellet M., Chan R., Mukhopadhyay A., Keasling J.D., Lee T.S. Identification and microbial production of a terpene-based advanced biofuel. Nat. Commun. 2011, 2:483.
23. Redding-Johanson A.M., Batth T.S., Chan R., Krupa R., Szmidt H.L., Adams P.D., Keasling J.D., Lee T.S., Mukhopadhyay A., Petzold C.J. Targeted proteomics for metabolic pathway optimization: application to terpene production. Metab. Eng. 2011, 13:194-203.
24. Roy S., Sahu A., Adhya S. Evolution of DNA binding motifs and operators. Gene 2002, 285:169-173.
25. Rude M.A., Baron T.S., Brubaker S., Alibhai M., Del Cardayre S.B., Schirmer A. Terminal olefin (1-alkene) biosynthesis by a novel p450 fatty acid decarboxylase from Jeotgalicoccus species. Appl. Environ. Microbiol. 2011, 77:1718-1727.
26. Schirmer A., Rude M.A., Li X., Popova E., del Cardayre S.B. Microbial biosynthesis of alkanes. Science 2010, 329:559-562.
27. Steen E.J., Kang Y., Bokinsky G., Hu Z., Schirmer A., McClure A., Del Cardayre S.B., Keasling J.D. Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Nature 2010, 463:559-562.
28. van Aalten D.M., DiRusso C.C., Knudsen J. The structural basis of acyl coenzyme A-dependent regulation of the transcription factor FadR. EMBO J. 2001, 20:2041-2050.
29. Wessel D., Flugge U.I. A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids. Anal. Biochem. 1984, 138:141-143.
30. Yu X., Liu T., Zhu F., Khosla C. In vitro reconstitution and steady-state analysis of the fatty acid synthase from Escherichia coli. Proc. Natl. Acad. Sci. U S A 2011, 108:18643-18648.
31. Zhang F., Rodriguez S., Keasling J.D. Metabolic engineering of microbial pathways for advanced biofuels production. Curr. Opin. Biotechnol. 2011, 22:775-783.