Design of an ectoine-responsive AraC mutant and its application in metabolic engineering of ectoine biosynthesis

Metabolic Engineering

Volumn 30, Issue , 2015, Pages 149-155

  Chen, Wei ^a   Zhang, Shan ^a   Jiang, Peixia ^a   Yao, Jun ^a,b   He, Yongzhi ^a   Chen, Lincai ^a   Gui, Xiwu ^c   Dong, Zhiyang ^a   Tang, Shuang Yan ^a  

^a INSTITUTE OF MICROBIOLOGY   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

^c SHANXI AGRICULTURAL UNIVERSITY   (China)

Author keywords

Directed evolution; Ectoine; High throughput screening; Metabolic engineering; Regulatory protein

Indexed keywords

BIOCHEMISTRY; BIOSYNTHESIS; CLONING; ESCHERICHIA COLI; METABOLISM; MOLECULES; PROTEINS; THROUGHPUT;

BIOSYNTHETIC PATHWAY; DIRECTED EVOLUTION; ECTOINE; HALOMONAS ELONGATA; HIGH THROUGHPUT SCREENING; HIGH THROUGHPUT SCREENING METHODS; RATE-LIMITING ENZYMES; REGULATORY PROTEIN;

METABOLIC ENGINEERING;

2,4 DIAMINOBUTYRIC ACID; AMINOTRANSFERASE; ARAC PROTEIN; ECTOINE; ARAC PROTEIN, E COLI; DIAMINO ACID; ESCHERICHIA COLI PROTEIN;

ARTICLE; BACTERIAL STRAIN; BACTERIUM MUTANT; CONTROLLED STUDY; ESCHERICHIA COLI; GENE ACTIVATION; GENE CLUSTER; GENETIC TRANSCRIPTION; HALOMONAS; HALOMONAS ELONGATA; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HIGH THROUGHPUT SCREENING; IN VITRO STUDY; METABOLIC ENGINEERING; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN BINDING; PROTEIN ENGINEERING; WILD TYPE; BIOSYNTHESIS; DIRECTED MOLECULAR EVOLUTION; GENETICS; METABOLISM; MUTATION; PROCEDURES;

ESCHERICHIA COLI; HALOMONAS ELONGATA;

AMINO ACIDS, DIAMINO; ARAC TRANSCRIPTION FACTOR; DIRECTED MOLECULAR EVOLUTION; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; METABOLIC ENGINEERING; MUTATION;

EID: 84935854110     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2015.05.004     Document Type: Article

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