Efficient gene-targeting in rat embryonic stem cells by CRISPR/Cas and generation of human kynurenine aminotransferase II (KAT II) knock-in rat

Transgenic Research

Volumn 24, Issue 6, 2015, Pages 991-1001

  Yamamoto, Satoshi ^a   Ooshima, Yuki ^a   Nakata, Mitsugu ^a   Yano, Takashi ^a   Nishimura, Naoya ^a   Nishigaki, Ryuuichi ^a   Satomi, Yoshinori ^a   Matsumoto, Hirokazu ^a   Matsumoto, Yoshio ^b   Takeyama, Michiyasu ^a  

^a TAKEDA PHARMACEUTICAL COMPANY LIMITED   (Japan)

^b Takeda Rabics Limited   (Japan)

Author keywords

CRISPR Cas; Embryonic stem (ES) cells; Genome editing; KAT II; Knock in; Rat

Indexed keywords

AMINOTRANSFERASE; KYNURENIC ACID; KYNURENINE-OXOGLUTARATE TRANSAMINASE; MESSENGER RNA;

ANIMAL; CELL CULTURE; CRISPR CAS SYSTEM; CYTOLOGY; EMBRYONIC STEM CELL; ENZYMOLOGY; FEMALE; GENE TARGETING; GENETICS; HOMOLOGOUS RECOMBINATION; HUMAN; LONG EVANS RAT; MALE; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; RAT; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEQUENCE HOMOLOGY; SOUTHERN BLOTTING; WISTAR RAT;

ANIMALS; BASE SEQUENCE; BLOTTING, SOUTHERN; CELLS, CULTURED; CRISPR-CAS SYSTEMS; EMBRYONIC STEM CELLS; FEMALE; GENE TARGETING; HOMOLOGOUS RECOMBINATION; HUMANS; KYNURENIC ACID; MALE; MOLECULAR SEQUENCE DATA; RATS; RATS, LONG-EVANS; RATS, WISTAR; REAL-TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SEQUENCE HOMOLOGY, NUCLEIC ACID; TRANSAMINASES;

EID: 84946402066     PISSN: 09628819     EISSN: 15739368     Source Type: Journal    
DOI: 10.1007/s11248-015-9909-1     Document Type: Article

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