Knockout rats via embryo microinjection of zinc-finger nucleases

Science

Volumn 325, Issue 5939, 2009, Pages 433-

  Geurts, Aron M ^a   Cost, Gregory J ^b   Freyvert, Yevgeniy ^b   Zeitler, Bryan ^b   Miller, Jeffrey C ^b   Choi, Vivian M ^b   Jenkins, Shirin S ^b   Wood, Adam ^c   Cui, Xiaoxia ^c   Meng, Xiangdong ^b   Vincent, Anna ^b   Lam, Stephen ^b   Michalkiewicz, Mieczyslaw ^a   Schilling, Rebecca ^a   Foeckler, Jamie ^b   Kalloway, Shawn ^b   Weiler, Hartmut ^a   Ménoret, Séverine ^d   Anegon, Ignacio ^d   Davis, Gregory D ^c   Zhang, Lei ^b   Rebar, Edward J ^b   Gregory, Philip D ^b   Urnov, Fyodor D ^b   Jacob, Howard J ^a   Buelow, Roland ^e   more..

^a MEDICAL COLLEGE OF WISCONSIN   (United States)

^b SANGAMO BIOSCIENCES INC   (United States)

^c Sigma Aldrich Co   (United States)

^d UNIVERSITÉ DE NANTES   (France)

^e Open Monoclonal Technology Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; IMMUNOGLOBULIN M; ZINC FINGER PROTEIN;

DNA; EMBRYO; ENZYME ACTIVITY; GENE EXPRESSION; MUTATION; RODENT; ZINC;

DNA REPAIR; DOUBLE STRANDED DNA BREAK; GENE DISRUPTION; GENE FUNCTION; GENE MUTATION; GENE TARGETING; GENOME; GERM LINE; MICROINJECTION; MUTAGENESIS; PRIORITY JOURNAL; RAT EMBRYO; SHORT SURVEY;

ANIMALS; BASE SEQUENCE; DNA; EMBRYO, MAMMALIAN; ENDODEOXYRIBONUCLEASES; FEASIBILITY STUDIES; FEMALE; GENE KNOCKOUT TECHNIQUES; GREEN FLUORESCENT PROTEINS; IMMUNOGLOBULIN M; MALE; MICROINJECTIONS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; RAB GTP-BINDING PROTEINS; RATS; RNA, MESSENGER; ZINC FINGERS;

RATTUS;

EID: 67749106611     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1172447     Document Type: Short Survey

References (8)

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4. Materials and methods are available as supporting material on Science Online.
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8. We thank R. Jaenisch, R. Hammer, P. Sullivan, and three anonymous referees for helpful suggestions; D. Smoller and E. Lanphier for support; E. Eastlund for the Rab38 ZFN mRNA; R. DeKelver and R. Amora for technical assistance; and Caliper Life Sciences, Incorporated for excellent service. Supported by NIH grants 5U01HL066579-08 and 5P01HL082798-03, a sponsored research agreement between the Medical College of Wisconsin and Sigma-Aldrich, and the American Physiological Society Fellowship in Physiological Genomics to A.M.G. The authors are filing patents based on the results reported in this paper.