CRISPR/Cas-induced double-strand breaks boost the frequency of gene replacements for humanizing the mouse Cnr2 gene

Biochemical and Biophysical Research Communications

Volumn 441, Issue 4, 2013, Pages 815-819

  Gennequin, Benjamin ^a   Otte, David Marian ^a   Zimmer, Andreas ^a  

^a UNIVERSITY OF BONN   (Germany)

Author keywords

Cannabinoid receptor; ES cells; Gene replacement; Knockout; Surveyor assay

Indexed keywords

CANNABINOID RECEPTOR; DOUBLE STRANDED RNA;

ALLELE; ARTICLE; CELL STRAIN 3T3; DOUBLE STRANDED DNA BREAK; ELECTROPORATION; EXON; GENE MUTATION; GENE TARGETING; GENETIC RECOMBINATION; HOMOLOGOUS RECOMBINATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

CANNABINOID RECEPTOR; ES CELLS; GENE REPLACEMENT; KNOCKOUT; SURVEYOR ASSAY;

ANIMALS; BASE SEQUENCE; CRISPR-CAS SYSTEMS; DNA BREAKS, DOUBLE-STRANDED; EXONS; GENETIC ENGINEERING; GENETIC LOCI; HUMANS; MICE; MOLECULAR SEQUENCE DATA; RECEPTOR, CANNABINOID, CB2; RECOMBINATION, GENETIC; RNA; TRANSFECTION;

EID: 84888848372     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2013.10.138     Document Type: Article

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