CFTR inactivation by lentiviral vector-mediated RNA interference and CRISPR-Cas9 genome editing in human airway epithelial cells

Current Gene Therapy

Volumn 15, Issue 5, 2015, Pages 447-459

  Bellec, Jessica ^a,b   Bacchetta, Marc ^c   Losa, Davide ^c   Anegon, Ignacio ^a,b   Chanson, Marc ^c   Nguyen, Tuan Huy ^a,b  

^a Centre National de la Recherche Scientifique   (France)

^b HÔTEL DIEU   (France)

^c GENEVA UNIVERSITY HOSPITALS   (Switzerland)

Author keywords

CFTR; CRISPR Cas9; Cystic fibrosis; Lentiviral vector; Primary cells; RNA interference

Indexed keywords

CRISPR ASSOCIATED PROTEIN; CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR; GENOMIC DNA; INTERLEUKIN 8; LENTIVIRUS VECTOR; SHORT HAIRPIN RNA; CFTR PROTEIN, HUMAN; SMALL INTERFERING RNA;

ARTICLE; CELL MIGRATION; CELL PROLIFERATION; CONTROLLED STUDY; CRISPR CAS SYSTEM; ELECTROPHYSIOLOGY; ENZYME LINKED IMMUNOSORBENT ASSAY; FLOW CYTOMETRY; FLUORESCENCE ACTIVATED CELL SORTING; HUMAN; HUMAN CELL; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN EXPRESSION; RESPIRATORY EPITHELIUM; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA INTERFERENCE; WESTERN BLOTTING; CELL LINE; CELL MOTION; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CYSTIC FIBROSIS; EPITHELIUM CELL; GENE EXPRESSION; GENE THERAPY; GENE VECTOR; GENETICS; GENOME; LENTIVIRINAE; METABOLISM; PHYSIOLOGY; PROCEDURES; RESPIRATORY SYSTEM;

CELL LINE; CELL MOVEMENT; CELL PROLIFERATION; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; CYSTIC FIBROSIS; CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR; EPITHELIAL CELLS; GENE EXPRESSION; GENETIC THERAPY; GENETIC VECTORS; GENOME; HUMANS; INTERLEUKIN-8; LENTIVIRUS; RESPIRATORY SYSTEM; RNA INTERFERENCE; RNA, SMALL INTERFERING;

EID: 84940902836     PISSN: 15665232     EISSN: 18755631     Source Type: Journal    
DOI: 10.2174/1566523215666150812115939     Document Type: Article

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