Establishment of astrocyte cell lines from sheep genetically susceptible to scrapie

In Vitro Cellular and Developmental Biology - Animal

Volumn 36, Issue 1, 2000, Pages 45-49

  Vilette, D ^a   Madelaine, M F ^a   Laude, H ^a  

^a INRA Institut National de la Recherche Agronomique   (France)

Author keywords

Cell line; Glial; Immortalization; Ovine; Prions; SV40

Indexed keywords

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL ISOLATION; CELL LINE; GENETIC SUSCEPTIBILITY; GENETIC TRANSFECTION; NONHUMAN; PATHOGENESIS; SCRAPIE;

ANIMALS; ASTROCYTES; CELL LINE; GENETIC PREDISPOSITION TO DISEASE; SCRAPIE; SHEEP;

ANIMALIA; OVIS; OVIS ARIES; RODENTIA; SIMIAE; SIMIAN VIRUS; SIMIAN VIRUS 40;

EID: 0034024162     PISSN: 10712690     EISSN: None     Source Type: Journal    
DOI: 10.1290/1071-2690(2000)036<0045:EOACLF>2.3.CO;2     Document Type: Article

References (34)

1. Anderson, C. A.; Higgins, R. J.; Waldvogel, A. S.; Osburn, B. I. Tropism of border disease virus for oligodendrocytes in ovine fetal brain cell-cultures. Am. J. Vet. Res. 48:822-827; 1987.
2. Bigami, A.; Dahl, D. Gliosis. In: Kettenmann, H.; Ransom, B. R., ed. Neuroglia. Oxford: Oxford University Press; 1995:843-858.
3. Brandner, S.; Isenmann, S.; Kühne, G.; Aguzzi, A. Identification of the end stage of scrapie using infected neural grafts. Brain Pathol. 8:19-27; 1998.
4. Brandner, S.; Isenmann, S.; Raeber, A.; Fisher, M.; Sailer, A.; Kobayashi, Y.; Marino, S.; Weissmann, C.; Aguzzi, A. Normal host prion protein necessary for scrapie-induced neurotoxicity. Nature 379:339-343; 1996.
5. Büeler, H.; Aguzzi, A.; Sailer, A.; Greiner, R. A.; Autenried, P.; Aguet, M.; Weissmann, C. Mice devoid of PrP are resistant to scrapie. Cell 73: 1339-1347; 1993.
6. Clouscard, C.; Beaudry, P.; Elsen, J. M.; Milan, D.; Dussaucy, M.; Bounneau, C.; Schelchert, F.; Chatelain, J.; Launay, J. M.; Laplanche, J. L. Different allelic effects of the codons 136 and 171 of the prion protein gene in sheep with natural scrapie. J. Gen. Virol. 76:2097-2101; 1995.
7. Diedrich, J. F.; Bendheim, P. E.; Kim, Y. S.; Carp, R. I.; Haase, A. T. Scrapie-associated prion protein accumulates in astrocytes during scrapie infection. Proc. Natl. Acad. Sci. USA 88:375-379; 1991.
8. Eklund, C. M.; Kennedy, R. C.; Hadlow, W. J. Pathogenesis of scrapie virus infection in the mouse. J. Infect. Dis. 117:15-22; 1967.
9. Elder, G. A.; Potts, B. J.; Sawyer, M. Characterization of glial subpopulations in cultures of the ovine central nervous system. Glia 1:317-327; 1988.
10. Eng, L. F. Glial fibrillary acidic protein (GFAP): the major protein of glial intermediate filaments in differentiated astrocytes. J. Neuroimmunol. 8:203-214; 1985.
11. Eng, L. F.; Lee, Y. E. Intermediate filaments in astrocytes. In: Kettenmann, H.; Ransom, B. R., ed. Neuroglia. Oxford: Oxford University Press; 1995:650-667.
12. Evrard, C.; Galiana, E.; Rouget, P. Establishment of "normal" nervous cell lines after transfert of polyoma virus and adenovirus early genes into murine brain cells. EMBO J. 5:3157-3162; 1986.
13. Fraser, H.; Dickinson, A. G. Pathogenesis of scrapie in the mouse: the role of the spleen. Nature 226:462-463; 1970.
14. Fedoroff, S.; White, R.; Subrahmanyan, L.; Kalnins V. I. Astrocyle cell lineage II. mouse fibrous astrocytes and reactive astrocytes in culture have vimentin- and GFP-containing intermediate filaments. Brain Res. 283:303-315; 1983.
15. Galiana, E.; Borde, I.; Marin, P.; Rassoulzadegan, M.; Cuzin, F.; Gros, F.; Rouget, P.; Evrard, C. Establishment of permanent astroglial cell lines, able to differentiate ex vivo, from transgenic mice carrying the polyomavirus large T gene: an alternative approach to brain cell immortalization. J. Neurosci. Res. 26:269-277; 1990.
16. Ghandour, M. S.; Labourdette, G.; Vincendon, G.; Gombos, G. A biochemical and immunohistological study of S100 protein in developing rat cerebellum. Dev. Neurosci. 4:98-109; 1981.
17. Gown, A. M.; Vogel, A. M. Monoclonal antibodies to intermediate filaments of human cells - unique and cross-reacting antibodies. J. Cell Biol. 95:414-424; 1982.
18. Harlow, E.; Crawford, L. V.; Pim, D. C.; Williamson, N. M. Monoclonal antibodies specific for simian virus 40 tumor antigens. J. Virol. 39:861-869; 1981.
19. Hunter, N. PrP genetics in sheep and the implications for scrapie and BSE. Trends Microbiol. 5:331-334; 1997.
20. Ludwin, S. K.; Szuchet, S. Myelination by mature oligodendrocytes in vivo and in vitro: evidence that different steps in the myelination process are independently controlled. Glia 8:219-231; 1993.
21. McCarthy, K. D.; de Vellis, J. Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue. J. Cell Biol. 85: 890-902; 1980.
22. Markovits, P.; Dormont, D.; Delpech, B.; Court, L.; Latarjet, R. Trials of ex vivo propagation of the scrapie agent in mouse nerve cells. C. R. Acad. Sci. Paris 293:413-417; 1981.
23. Moser, M.; Collelo, R. J., Pott, U.; Oesch, B. Developmental expression of the prion protein gene in glial cells. Neuron 14:509-517; 1995.
24. Oblinger, M. M.; Kost, S. A.; Singh, L. D. In: Federoff, S.; Juurlink, B. M. J.; Doucette, R., ed. Biology and pathology of astrocyte-neuron interactions. Plenum Press, New York: 1993:291-302.
25. Prusiner, S. B. Molecular biology and pathogenesis of prion diseases. Trends Biochem. Sci. 21:482-487; 1996.
26. Quinlan, R. A.; Franke, W. W. Molecular interactions in intermediate-sized filaments revealed by chemical crosslinking: heteropolymers of vimentin and glial fibrillary acidic protein in cultured human glioma cells. Eur. J. Biochem. 132:477-484; 1983.
27. Race, R. The scrapie agent ex vivo. Curr. Top. Microbiol. Immunol. 172:181-193; 1991.
28. Raeber, A. J.; Race, R. E.; Brandner, S.; Priola, S. A.; Sailer, A.; Bessen, R. A.; Mucke, L.; Manson, J.; Aguzzi, A.; Oldstone, M. B. A.; Weissmann, C.; Chesebro, B. Astrocyte-specific expression of hamster prion protein (PrP) renders PrP knockout mice susceptible to hamster scrapie. EMBO J. 16:6057-6065; 1997.
29. Richard, O.; Duitloz, A. H.; Hevor, T. K. Early, middle, and late stages of neural cells from ovine embryo in primary cultures. Neurosci. Res. 31:61-68; 1998.
30. Sailer, A.; Büeler, H.; Fisher, M.; Aguzzi, A.; Weissmann, C. No propagation of prions in mice devoid of PrP. Cell 77:967-968; 1994.
31. Schuermann, M. An expression vector for stable expression of oncogenes. Nucleic Acids Res. 18:4945-4946; 1990.
32. Schwartz, B.; Vicard, P. Delouis, C.; Paulin, D. Mammalian cell lines can be efficiently established ex vivo upon expression of the SV40 large T antigen driven by a promoter sequence derived from the human vimentin gene. Biol. Cell. 73:7-14; 1991.
33. Sharp, G.; Osborn, M.; Weber, K. Occurrence of two different intermediate filament proteins in the same filament in situ within a human glioma cell line. An immunoelectron microscopical study. Exp. Cell Res. 141:385-395; 1982.
34. Wang, E.; Cairncross, J. G.; Liem, R. K. H. Identification of glial filament protein and vimentin in the same intermediate filament system in human glioma cells. Proc. Natl. Acad. Sci. USA 81:2102-2106; 1984.