Transcription yield of fully 2'-modified RNA can be increased by the addition of thermostabilizing mutations to T7 RNA polymerase mutants

Nucleic Acids Research

Volumn 43, Issue 15, 2015, Pages 7480-7488

  Meyer, Adam J ^a   Garry, Daniel J ^a   Hall, Bradley ^b   Byrom, Michelle M ^a   McDonald, Hannah G ^a   Yang, Xu ^c   Yin, Y Whitney ^c   Ellington, Andrew D ^a  

^a UNIVERSITY OF TEXAS AT AUSTIN   (United States)

^b Altermune Technologies   (United States)

^c University of Texas Medical Branch School of Medicine   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA DIRECTED RNA POLYMERASE; MUTANT PROTEIN; RECOMBINANT RNA; T7 RNA POLYMERASE; UNCLASSIFIED DRUG; BACTERIOPHAGE T7 RNA POLYMERASE; RNA; VIRUS PROTEIN;

ARTICLE; CONCENTRATION (PARAMETERS); CRYSTALLIZATION; ENZYME ACTIVITY; ENZYME SPECIFICITY; ENZYME STABILITY; GENETIC TRANSCRIPTION; MUTATION; PRIORITY JOURNAL; BIOSYNTHESIS; CHEMISTRY; GENETICS; METABOLISM; TEMPERATURE;

DNA-DIRECTED RNA POLYMERASES; ENZYME STABILITY; MUTATION; RNA; SUBSTRATE SPECIFICITY; TEMPERATURE; TRANSCRIPTION, GENETIC; VIRAL PROTEINS;

EID: 84941585619     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gkv734     Document Type: Article

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