In vivo activity of nuclease-resistant siRNAs

RNA

Volumn 10, Issue 5, 2004, Pages 766-771

  Layzer, Juliana M ^a   McCaffrey, Anton P ^b   Tanner, Alice K ^a   Huang, Zan ^b   Kay, Mark A ^b   Sullenger, Bruce A ^a  

^a DUKE UNIVERSITY MEDICAL CENTER   (United States)

^b STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Luciferase; Modified nucleotides; RNAi; SiRNA

Indexed keywords

LUCIFERASE; NUCLEASE; NUCLEOTIDE; SMALL INTERFERING RNA;

ANIMAL CELL; ARTICLE; BIOLUMINESCENCE; CELL STRAIN 3T3; CHEMICAL MODIFICATION; GENE EXPRESSION; HALF LIFE TIME; HYDRODYNAMICS; MOUSE; NONHUMAN; PRIORITY JOURNAL; RNA INTERFERENCE; RNA STABILITY;

ANIMALS; ENDONUCLEASES; LUMINESCENT MEASUREMENTS; MICE; RNA, SMALL INTERFERING; TRANSFECTION;

ANIMALIA; MAMMALIA;

EID: 1942533474     PISSN: 13558382     EISSN: None     Source Type: Journal    
DOI: 10.1261/rna.5239604     Document Type: Article

References (27)

1. Bernstein, E., Caudy, A.A., Hammond, S.M., and Hannon, G.J. 2001. Role for a bidentate ribonuclease in the initiation step of RNA interference. Nature 409: 363-366.
2. Braasch, D.A., Jensen, S., Liu, Y., Kaur, K., Arar, K., White, M.A., and Corey, D.R. 2003. RNA interference in mammalian cells by chemically-modified RNA. Biochemistry 42: 7967-7975.
3. Chiu, Y.L. and Rana, T.M. 2003. siRNA function in RNAi: A chemical modification analysis. RNA 9: 1034-1048.
4. Contag, C.H., Spilman, S.D., Contag, P.R., Oshiro, M., Eames, B., Dennery, P., Stevenson, D.K., and Benaron, D. A. 1997. Visualizing gene expression in living mammals using a bioluminescent reporter. Photochem. Photobiol. 66: 523-531.
5. Czauderna, F., Fechtner, M., Dames, S., Aygun, H., Klippel, A., Pronk, G.J., Giese, K., and Kaufmann, J. 2003. Structural variations and stabilising modifications of synthetic siRNAs in mammalian cells. Nucleic Acids Res. 31: 2705-2716.
6. Dykxhoorn, D.M., Novina, C.D., and Sharp, P.A. 2003. Killing the messenger: Short RNAs that silence gene expression. Nat. Rev. Mol. Cell. Biol. 4: 457-467.
7. Elbashir, S.M., Harborth, J., Lendeckel, W., Yalcin, A., Weber, K., and Tuschl, T. 2001. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 411: 494-498.
8. Fire, A., Xu, S., Montgomery, M.K., Kostas, S.A., Driver, S.E., and Mello, C.C. 1998. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391: 806-811.
9. Hamilton, A.J. and Baulcombe, D.C. 1999. A species of small antisense RNA in posttranscriptional gene silencing in plants. Science 286: 950-952.
10. Hammond, S.M., Bernstein, E., Beach, D., and Hannon, G.J. 2000. An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells. Nature 404: 293-296.
11. Hannon, G.J. 2002. RNA interference. Nature 418: 244-251.
12. Harborth, J., Elbashir, S.M., Vandenburgh, K., Manninga, H., Scaringe, S.A., Weber, K., and Tuschl, T. 2003. Sequence, chemical, and structural variation of small interfering RNAs and short hairpin RNAs and the effect on mammalian gene silencing. Antisense Nucleic Acid Drug Dev. 13: 83-105.
13. Lee, N.S., Dohjima, T., Bauer, G., Li, H., Li, M.J., Ehsani, A., Salvaterra, P., and Rossi, J. 2002. Expression of small interfering RNAs targeted against HIV-1 rev transcripts in human cells. Nat. Biotechnol. 20: 500-505.
14. Liu, F., Song, Y., and Liu, D. 1999. Hydrodynamics-based transfection in animals by systemic administration of plasmid DNA. Gene Ther. 6: 1258-1266.
15. McCaffrey, A.P., Meuse, L., Pham, T.T., Conklin, D.S., Hannon, G.J., and Kay, M.A. 2002a. RNA interference in adult mice. Nature 418: 38-39.
16. McCaffrey, A.P., Ohashi, K., Meuse, L., Shen, S., Lancaster, A.M., Lukavsky, P.J., Sarnow, P., and Kay, M.A. 2002b. Determinants of hepatitis C translational initiation in vitro, in cultured cells and mice. Mol. Ther. 5: 676-684.
17. McCaffrey, A.P., Nakai, H., Pandey, K., Huang, Z., Salazar, F.H., Xu, H., Wieland, S.F., Marion, P.L., and Kay, M.A. 2003. Inhibition of hepatitis B virus in mice by RNA interference. Nat. Biotechnol. 21: 639-644.
18. McManus, M.T. and Sharp, P.A. 2002. Gene silencing in mammals by small interfering RNAs. Nat. Rev. Genet. 3: 737-747.
19. Miyagishi, M. and Taira, K. 2002. U6 promoter-driven siRNAs with four uridine 3′ overhangs efficiently suppress targeted gene expression in mammalian cells. Nat. Biotechnol. 20: 497-500.
20. Paul, C.P., Good, P.D., Winer, I., and Engelke, D.R. 2002. Effective expression of small interfering RNA in human cells. Nat. Biotechnol. 20: 505-508.
21. Pieken, W.A., Olsen, D.B., Benseler, F., Aurup, H., and Eckstein, F. 1991. Kinetic characterization of ribonuclease-resistant 2′-modified hammerhead ribozymes. Science 253: 314-317.
22. Rusconi, C.P., Scardino, E., Layzer, J., Pitoc, G.A., Ortel, T.L., Monroe, D., and Sullenger, B.A. 2002. RNA aptamers as reversible antagonists of coagulation factor IXa. Nature 419: 90-94.
23. Song, E., Lee, S.K., Dykxhoorn, D.M., Novina, C., Zhang, D., Crawford, K., Cerny, J., Sharp, P.A., Lieberman, J., Manjunath, N., et al. 2003. Sustained small interfering RNA-mediated human immunodeficiency virus type 1 inhibition in primary macrophages. J. Virol. 77: 7174-7181.
24. Sui, G., Soohoo, C., Affar el, B., Gay, F., Shi, Y., and Forrester, W.C. 2002. A DNA vector-based RNAi technology to suppress gene expression in mammalian cells. Proc. Natl. Acad. Sci. 99: 5515-5520.
25. Yant, S.R., Meuse, L., Chiu, W., Ivics, Z., Izsvak, Z., and Kay, M.A. 2000. Somatic integration and long-term transgene expression in normal and haemophilic mice using a DNA transposon system. Nat. Genet. 25: 35-41.
26. Zamore, P.D., Tuschl, T., Sharp, P.A., and Bartel, D.P. 2000. RNAi: Double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals. Cell 101: 25-33.
27. Zhang, G., Budker, V., and Wolff, J.A. 1999. High levels of foreign gene expression in hepatocytes after tail vein injections of naked plasmid DNA. Hum. Gene Ther. 10: 1735-1737.