Identification of a T7 RNA polymerase variant that permits the enzymatic synthesis of fully 2'-O-methyl-modified RNA

Journal of Biotechnology

Volumn 167, Issue 3, 2013, Pages 287-295

  Ibach, Jenny ^b   Dietrich, Laura ^a   Koopmans, Kyra R M ^a   Nöbel, Nico ^a   Skoupi, Marc ^c   Brakmann, Susanne ^a  

^a TU DORTMUND UNIVERSITY   (Germany)

^b MAX PLANCK INSTITUTE OF MOLECULAR PHYSIOLOGY   (Germany)

^c KARLSRUHE INSTITUTE OF TECHNOLOGY   (Germany)

Author keywords

Aptamer; Modified nucleotide; Polymerase; RNA

Indexed keywords

APTAMERS; BIOTECHNOLOGICAL APPLICATIONS; MODIFIED NUCLEOTIDES; PHARMACOKINETIC PROPERTIES; POLYMERASE; POLYMERASE ACTIVITIES; PROTEIN EXPRESSIONS; SUBSTRATE SPECIFICITY;

NUCLEOTIDES; POLYMERS; SUBSTRATES;

RNA;

2' O METHYL MODIFIED RNA; NUCLEIC ACID; NUCLEOTIDE; RNA; RNA POLYMERASE; T7 RNA POLYMERASE; UNCLASSIFIED DRUG; APTAMER; BACTERIAL RNA; BACTERIOPHAGE T7 RNA POLYMERASE; DNA DIRECTED RNA POLYMERASE; EPIDERMAL GROWTH FACTOR RECEPTOR; NUCLEOSIDE; VIRUS PROTEIN;

ARTICLE; BIOCATALYST; ENZYME ACTIVITY; ENZYME SPECIFICITY; ENZYME SYNTHESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; SCREENING; BIOTECHNOLOGY; CHEMISTRY; ESCHERICHIA COLI; GENETICS; HUMAN; METABOLISM; MUTATION; PLASMID; SITE DIRECTED MUTAGENESIS;

APTAMERS, NUCLEOTIDE; BIOTECHNOLOGY; DNA-DIRECTED RNA POLYMERASES; ESCHERICHIA COLI; HUMANS; MUTAGENESIS, SITE-DIRECTED; MUTATION; NUCLEOSIDES; PLASMIDS; RECEPTOR, EPIDERMAL GROWTH FACTOR; RNA, BACTERIAL; VIRAL PROTEINS;

EID: 84881513084     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2013.07.005     Document Type: Article

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