Structure of a transcribing T7 RNA polymerase initiation complex

Science

Volumn 286, Issue 5448, 1999, Pages 2305-2309

  Cheetham, Graham M T ^a   Steitz, Thomas A ^a,b  

^a YALE UNIVERSITY   (United States)

^b HOWARD HUGHES MEDICAL INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

HETERODUPLEX; LYSOZYME; RNA; RNA POLYMERASE; TRINUCLEOTIDE;

ARTICLE; BACTERIOPHAGE T7; CONFORMATIONAL TRANSITION; ENZYME CONFORMATION; ENZYME REGULATION; ENZYME SPECIFICITY; ENZYME SUBSTRATE COMPLEX; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN DNA BINDING; RNA SYNTHESIS; TRANSCRIPTION INITIATION;

AMINO ACID MOTIFS; BACTERIOPHAGE T7; CATALYTIC DOMAIN; CONSERVED SEQUENCE; CRYSTALLOGRAPHY, X-RAY; DNA, SINGLE-STRANDED; DNA-DIRECTED DNA POLYMERASE; DNA-DIRECTED RNA POLYMERASES; HYDROGEN BONDING; MODELS, MOLECULAR; N-ACETYLMURAMOYL-L-ALANINE AMIDASE; NUCLEIC ACID CONFORMATION; NUCLEIC ACID HETERODUPLEXES; OLIGORIBONUCLEOTIDES; PROMOTER REGIONS (GENETICS); PROTEIN CONFORMATION; PROTEIN STRUCTURE, TERTIARY; RNA, MESSENGER; SUBSTRATE SPECIFICITY; TEMPLATES, GENETIC; TRANSCRIPTION, GENETIC; VIRAL PROTEINS;

EID: 0033579380     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.286.5448.2305     Document Type: Article

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42. We thank J. Ippolito, D. Kennedy, and J. Wang for discussions and P. Eatherton for assistance in the preparation of this manuscript. This research was supported by NIH grant number CM-22778 (T.A.S.). The complete refined coordinates of the T7 RNAP transcribing complex and the observed structure factor amplitudes have been deposited in the Protein Data Bank with numbers 1QLN and R1QLNSF.