A multi-pronged investigation into the effect of glucose starvation and culture duration on fed-batch CHO cell culture

Biotechnology and Bioengineering

Volumn 112, Issue 10, 2015, Pages 2172-2184

  Fan, Yuzhou ^a,b   Jimenez Del Val, Ioscani ^c   Müller, Christian ^b   Lund, Anne Mathilde ^a   Sen, Jette Wagtberg ^b   Rasmussen, Søren Kofoed ^b   Kontoravdi, Cleo ^c   Baycin Hizal, Deniz ^d   Betenbaugh, Michael J ^d   Weilguny, Dietmar ^b   Andersen, Mikael Rørdam ^a  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^b Symphogen AS   (Denmark)

^c IMPERIAL COLLEGE LONDON   (United Kingdom)

^d Johns Hopkins University   (United States)

Author keywords

Chinese hamster ovary cells; Culture duration; Fed batch; Glucose starvation; Glycosylation; Monoclonal antibody; Proteomics

Indexed keywords

ANTIBODIES; CELL CULTURE; CELL DEATH; CELLS; CYTOLOGY; ENZYME ACTIVITY; GLUCOSE; GLYCOSYLATION; METABOLISM; MOLECULAR BIOLOGY; MONOCLONAL ANTIBODIES; PHYSIOLOGY; PRODUCTIVITY; PROTEINS; QUALITY CONTROL;

CHINESE HAMSTER OVARY CELLS; COMPARATIVE PROTEOMICS; EXTRACELLULAR MATRICES; FED BATCHES; INTRACELLULAR CONCENTRATION; MONOCLONAL ANTIBODIES (MAB); PROTEOMICS; SPECIFIC PRODUCTIVITY;

BATCH CELL CULTURE;

GLUCOSE; MONOCLONAL ANTIBODY; CULTURE MEDIUM; RECOMBINANT PROTEIN;

ANIMAL CELL; ANTIBODY PRODUCTION; ANTIBODY TITER; APOPTOSIS; ARTICLE; CELL METABOLISM; CONTROLLED STUDY; EXTRACELLULAR MATRIX; FED BATCH CULTURE; GLUCOSE METABOLISM; GLYCOSYLATION; NONHUMAN; PROTEIN SECRETION; PROTEOMICS; ANIMAL; BIOSYNTHESIS; CELL CULTURE TECHNIQUE; CHEMISTRY; CHO CELL LINE; CRICETULUS; CULTURE MEDIUM; GENETICS; METABOLISM; PROCEDURES;

CRICETULUS GRISEUS;

ANIMALS; ANTIBODIES, MONOCLONAL; CELL CULTURE TECHNIQUES; CHO CELLS; CRICETULUS; CULTURE MEDIA; GLUCOSE; RECOMBINANT PROTEINS;

EID: 84940467009     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25620     Document Type: Article

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