The availability of glucose to CHO cells affects the intracellular lipid-linked oligosaccharide distribution, site occupancy and the N-glycosylation profile of a monoclonal antibody

Journal of Biotechnology

Volumn 170, Issue 1, 2014, Pages 17-27

  Liu, Bo ^a   Spearman, Maureen ^a   Doering, John ^a   Lattová, Erica ^a   Perreault, Hélène ^a   Butler, Michael ^a  

^a UNIVERSITY OF MANITOBA   (Canada)

Author keywords

CHO cells; Glycosylation; Lipid linked oligosaccharide; Monoclonal antibody

Indexed keywords

CHO CELL; FED-BATCH CULTURES; GLUCOSE CONCENTRATION; HEAVY-CHAIN ANTIBODIES; HUMANIZED ANTIBODY; LOW MOLECULAR WEIGHT; MICROHETEROGENEITY; STEADY-STATE LEVEL;

BATCH CELL CULTURE; CELLS; CYTOLOGY; ESTERIFICATION; GLYCOSYLATION; MONOCLONAL ANTIBODIES; OLIGOSACCHARIDES; POLYSACCHARIDES; SUBSTRATES;

GLUCOSE;

GALACTOSE; GLUCOSE; GLYCAN; LIPOOLIGOSACCHARIDE; MANGANESE; MONOCLONAL ANTIBODY; URIDINE;

ANIMAL CELL; ANIMAL CELL CULTURE; ARTICLE; CELL DENSITY; CELLULAR DISTRIBUTION; CHO CELL; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CULTURE MEDIUM; FEMALE; GLUCOSE METABOLISM; GLUCOSE TRANSPORT; NONHUMAN; PRIORITY JOURNAL; PROTEIN GLYCOSYLATION; PROTEIN POLYMORPHISM; RAT; SIALYLATION; STEADY STATE;

CHO CELLS; GLYCOSYLATION; LIPID-LINKED OLIGOSACCHARIDE; MONOCLONAL ANTIBODY;

ANIMALS; ANTIBODIES, MONOCLONAL; CHO CELLS; CRICETULUS; CULTURE MEDIA; GALACTOSE; GLUCOSE; GLYCOSYLATION; LIPOPOLYSACCHARIDES; RECOMBINANT PROTEINS;

EID: 84890158776     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2013.11.007     Document Type: Article

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