An optimized method for extraction and quantification of nucleotides and nucleotide sugars from mammalian cells

Analytical Biochemistry

Volumn 443, Issue 2, 2013, Pages 172-180

  Del Val, Ioscani Jimenez ^a   Kyriakopoulos, Sarantos ^a   Polizzi, Karen M ^a   Kontoravdi, Cleo ^a  

^a IMPERIAL COLLEGE LONDON   (United Kingdom)

Author keywords

CHO cells; HPLC; Metabolite extraction; Nucleotide sugars; Nucleotides; Protein glycosylation

Indexed keywords

CELL CULTURE; CELLS; CHROMATOGRAPHIC ANALYSIS; CHROMATOGRAPHY; GLYCOSYLATION; MAMMALS; METABOLISM; METABOLITES; NUCLEOTIDES; PROTEINS; QUENCHING; SUGARS; WASHING;

CHO CELL; HPLC; METABOLITE EXTRACTION; NUCLEOTIDE SUGAR; PROTEIN GLYCOSYLATION;

EXTRACTION;

ACETONITRILE; ADENOSINE TRIPHOSPHATE; CYTIDINE TRIPHOSPHATE; GUANOSINE TRIPHOSPHATE; NUCLEOTIDE; URIDINE TRIPHOSPHATE;

ANIMAL CELL; ANION EXCHANGE; ARTICLE; CELL CULTURE; CHROMATOGRAPHY; EXTRACTION; LIMIT OF DETECTION; LIMIT OF QUANTITATION; MAMMAL CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN GLYCOSYLATION;

MAMMALIA;

CHO CELLS; HPLC; METABOLITE EXTRACTION; NUCLEOTIDE SUGARS; NUCLEOTIDES; PROTEIN GLYCOSYLATION;

ANIMALS; CARBOHYDRATES; CHO CELLS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CRICETULUS; GLYCOSYLATION; NUCLEOTIDES;

EID: 84886072423     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2013.09.005     Document Type: Article

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