Effects of cell culture conditions on antibody N-linked glycosylation-what affects high mannose 5 glycoform

Biotechnology and Bioengineering

Volumn 108, Issue 10, 2011, Pages 2348-2358

  Pacis, Efren ^a,b   Yu, Marcella ^a,b,c   Autsen, Jennifer ^a,b   Bayer, Robert ^a,b   Li, Feng ^a,b  

^a Oceanside Pharma Technical Development   (United States)

^b GENENTECH INC   (United States)

^c GENZYME CORPORATION   (United States)

Author keywords

Chinese hamster ovary; Culture duration; Glycosylation; Man5; Manganese chloride; Osmolality

Indexed keywords

CELL CULTURE MEDIUMS; CELL CULTURE PROCESS; CELL LINES; CHINESE HAMSTER OVARY; CHINESE HAMSTER OVARY CELLS; CULTURE CONDITIONS; FED-BATCH PROCESS; FOLLOW-UP STUDIES; GLYCOFORMS; GLYCOSYLTRANSFERASES; MAN5; MANGANESE CHLORIDE; MRNA LEVEL; N-LINKED; OSMOLALITY; PROCESS PARAMETERS; THERAPEUTIC ANTIBODIES; THERAPEUTIC PRODUCTS;

ANTIBODIES; BATCH DATA PROCESSING; CELLS; CURRICULA; ENZYME ACTIVITY; ESTERIFICATION; GLYCOSYLATION; MANGANESE; SODIUM CHLORIDE;

CELL CULTURE;

MANGANESE CHLORIDE; MANNOSE; MESSENGER RNA; N ACETYLGLUCOSAMINYLTRANSFERASE; RECOMBINANT ANTIBODY; SODIUM CHLORIDE; CULTURE MEDIUM; MONOCLONAL ANTIBODY; RECOMBINANT PROTEIN;

ANIMAL CELL; ANTIBODY PRODUCTION; ARTICLE; CELL CULTURE; CELL LINE; ENZYME ACTIVITY; FED BATCH CULTURE; FOLLOW UP; GLYCOSYLATION; NONHUMAN; OSMOLALITY; ANIMAL; BIOSYNTHESIS; CHEMISTRY; CHO CELL LINE; CRICETULUS; CULTURE MEDIUM; CULTURE TECHNIQUE; HAMSTER; MOUSE; OSMOLARITY;

CRICETULUS GRISEUS;

ANIMALS; ANTIBODIES, MONOCLONAL, MURINE-DERIVED; CELL CULTURE TECHNIQUES; CHO CELLS; CRICETINAE; CRICETULUS; CULTURE MEDIA; GLYCOSYLATION; MANNOSE; MICE; OSMOLAR CONCENTRATION; RECOMBINANT PROTEINS;

EID: 80051798475     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.23200     Document Type: Article

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