A versatile, efficient strategy for assembly of multi-fragment expression vectors in Saccharomyces cerevisiae using 60 bp synthetic recombination sequences

Microbial Cell Factories

Volumn 12, Issue 1, 2013, Pages

  Kuijpers, Niels G A ^a,b   Solis Escalante, Daniel ^a,b   Bosman, Lizanne ^a,b   van den Broek, Marcel ^a,b   Pronk, Jack T ^a,b,c   Daran, Jean Marc ^a,b,c   Daran Lapujade, Pascale ^a,b  

^a DELFT UNIVERSITY OF TECHNOLOGY   (Netherlands)

^b KLUYVER CENTRE FOR GENOMICS OF INDUSTRIAL FERMENTATION   (Netherlands)

^c Platform for Green Synthetic Biology   (Netherlands)

Author keywords

Homologous recombination; In vivo assembly; Pathway engineering; Saccharomyces cerevisiae; Synthetic biology

Indexed keywords

DNA FRAGMENT; PLASMID DNA;

ARTICLE; EPISOME; EXPRESSION VECTOR; GENE AMPLIFICATION; GENE EXPRESSION; GENE REPLICATION; GENE SEQUENCE; GENE VECTOR; GENETIC RECOMBINATION; IN VIVO STUDY; PLASMID; POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE; SURVIVAL; YEAST;

BASE SEQUENCE; GENETIC VECTORS; HOMOLOGOUS RECOMBINATION; METABOLIC ENGINEERING; MULTIPLEX POLYMERASE CHAIN REACTION; PLASMIDS; SACCHAROMYCES CEREVISIAE;

SACCHAROMYCES CEREVISIAE;

EID: 84877272995     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/1475-2859-12-47     Document Type: Article

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