Mouse genome editing using the CRISPR/Cas system

Current Protocols in Human Genetics

Volumn 83, Issue , 2014, Pages 15.7.1-15.7.27

  Harms, Donald W ^a   Quadros, Rolen M ^a   Seruggia, Davide ^b,c   Ohtsuka, Masato ^d   Takahashi, Gou ^d,e   Montoliu, Lluis ^b,c   Gurumurthy, Channabasavaiah B ^a  

^a UNIVERSITY OF NEBRASKA MEDICAL CENTER   (United States)

^b CENTRO NACIONAL DE BIOTECNOLOGÍA   (Spain)

^c INSTITUTO DE SALUD CARLOS III   (Spain)

^d TOKAI UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^e TOKYO UNIVERSITY OF AGRICULTURE   (Japan)

Author keywords

CRISPR Cas9; Gene editing; Mutant mouse; Pronuclear and cytoplasmic injection; sgRNA

Indexed keywords

CASPASE 9; DNA; MESSENGER RNA; PLASMID DNA; REAGENT; RNA;

ARTICLE; CELL ISOLATION; CLONING; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; DNA CONTENT; DNA REPAIR; DNA TEMPLATE; EMBRYO TRANSFER; EMBRYONIC STEM CELL; FERTILIZATION; GENE MUTATION; GENE SEQUENCE; GENETIC ENGINEERING; GENETICS; GENOTYPE; IN VITRO STUDY; KNOCK IN MOUSE; KNOCKOUT MOUSE; NONHUMAN; PLASMID; POLYADENYLATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROGENY; PROMOTER REGION; PSEUDOPREGNANCY; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; RNA EDITING; RNA PURIFICATION; RNA SYNTHESIS; RNA TRANSCRIPTION; RNA TRANSLATION; SUPEROVULATION; ANIMAL; GENOME; MOUSE;

ANIMALS; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; GENOME; MICE;

EID: 84934904398     PISSN: 19348266     EISSN: 19348258     Source Type: Journal    
DOI: 10.1002/0471142905.hg1507s83     Document Type: Article

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