Generation of targeted mouse mutants by embryo microinjection of TALEN mRNA

Nature Protocols

Volumn 8, Issue 12, 2013, Pages 2355-2379

  Wefers, Benedikt ^a   Panda, Sudeepta K ^a,b   Ortiz, Oskar ^a   Brandl, Christina ^a,b   Hensler, Svenja ^a,b   Hansen, Jens ^a   Wurst, Wolfgang ^a,b,c,d   Kühn, Ralf ^a,b  

^a INSTITUTE OF EPIDEMIOLOGY   (Germany)

^b TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^c GERMAN CENTER FOR NEURODEGENERATIVE DISEASES DZNE   (Germany)

^d MAX PLANCK INSTITUTE OF PSYCHIATRY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVATING TRANSCRIPTION FACTOR; MESSENGER RNA; NUCLEASE; OLIGODEOXYNUCLEOTIDE; TRANSCRIPTION ACTIVATOR LIKE EFFECTOR NUCLEASE; UNCLASSIFIED DRUG;

ARTICLE; BINDING AFFINITY; BINDING SITE; DNA MODIFICATION; DNA SEQUENCE; ENZYME ACTIVITY; GENE DELETION; GENE INACTIVATION; GENE SEQUENCE; GENE TARGETING; GENETIC VARIABILITY; GENETICALLY ENGINEERED MOUSE STRAIN; MESSENGER RNA SYNTHESIS; MICROINJECTION; MUTAGENESIS; NONHUMAN; NUCLEAR LOCALIZATION SIGNAL; PRIORITY JOURNAL; PROGENY; PROMOTER REGION; PROTEIN DNA BINDING; PROTEIN EXPRESSION; SINGLE NUCLEOTIDE POLYMORPHISM;

MAMMALIA; MUS;

ANIMALS; BINDING SITES; DEOXYRIBONUCLEASES; EMBRYO, MAMMALIAN; GENE TARGETING; GENETIC ENGINEERING; GENOTYPING TECHNIQUES; MICE; MICE, KNOCKOUT; MICROINJECTIONS; MUTAGENESIS, SITE-DIRECTED; OLIGODEOXYRIBONUCLEOTIDES; RNA, MESSENGER;

EID: 84888338529     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2013.142     Document Type: Article

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