Composition of the early intestinal microbiota:Knowledge, knowledge gaps and the use of high-throughput sequencing to address these gaps

Gut Microbes

Volumn 3, Issue 3, 2012, Pages 203-220

  Fouhy, Fiona ^a,b   Ross, R Paul ^a,c   Fitzgerald, Gerald ^b,c   Stanton, Catherine ^a,c   Cotter, Paul D ^a,c  

^a TEAGASC FOOD RESEARCH CENTRE   (Ireland)

^b UNIVERSITY COLLEGE CORK   (Ireland)

^c UNIVERSITY COLLEGE CORK   (Ireland)

Author keywords

Antibiotics; Colonization; Gut microbiota; High throughput sequencing; Infant; Prebiotics; Probiotics

Indexed keywords

ANTIBIOTIC AGENT; LACTOFERRIN; PREBIOTIC AGENT; PROBIOTIC AGENT;

BACTERIAL COLONIZATION; BACTERIUM CULTURE; FEEDING; HIGH THROUGHPUT SEQUENCING; HUMAN; INTESTINE FLORA; MATERNAL NUTRITION; NONHUMAN; PERINATAL DRUG EXPOSURE; PRENATAL DRUG EXPOSURE; REVIEW; AGE; BIOTA; GASTROINTESTINAL TRACT; INFANT; METHODOLOGY; MICROBIOLOGY; NEWBORN;

AGE FACTORS; BIOTA; GASTROINTESTINAL TRACT; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HUMANS; INFANT; INFANT, NEWBORN;

EID: 84930477508     PISSN: 19490976     EISSN: 19490984     Source Type: Journal    
DOI: 10.4161/gmic.20169     Document Type: Review

References (181)

1. Adlerberth I, Wold AE. Establishment of the gut microbiota in western infants. Acta Paediatr 2009; 98:229-238. PMID:19143664; http://dx.doi.org/10.1111/j.1651-2227.2008.01060.x.
2. Vaishampayan PA, Kuehl JV, Froula JL, Morgan JL, Ochman H, Francino MP. Comparative metagenomics and population dynamics of the gut microbiota in mother and infant. Genome Biol Evol 2010; 2:53-66. PMID:20333224; http://dx.doi.org/10.1093/gbe/evp057.
3. Palmer C, Bik EM, DiGiulio DB, Relman DA, Brown PO. Development of the human infant intestinal microbiota. PLoS Biol 2007; 5:177. PMID:17594176; http://dx.doi.org/10.1371/journal.pbio.0050177.
4. Fagerås M, Tomičić S, Voor T, Björkstén B, Jenmalm MC. Slow salivary secretory IgA maturation may relate to low microbial pressure and allergic symptoms in sensitized children. Pediatr Res 2011; 70:572-577. PMID:21857384; http://dx.doi.org/10.1203/PDR.0b013e318232169e.
5. Conroy ME, Shi HN, Walker WA. The long-term health effects of neonatal microbial flora. Curr Opin Allergy Clin Immunol 2009; 9:197-201. PMID:19398905; http://dx.doi.org/10.1097/ACI.0b013e32832b3f1d.
6. O'Hara AM, Shanahan F. The gut flora as a forgotten organ. EMBO Rep 2006; 7:688-693. PMID:16819463; http://dx.doi.org/10.1038/sj.embor.7400731.
7. Guarner F, Malagelada JR. Gut flora in health and disease. Lancet 2003; 361:512-519. PMID:12583961; http://dx.doi.org/10.1016/S0140-6736(03)12489-0.
8. Eckburg PB, Bik EM, Bernstein CN, Purdom E, Dethlefsen L, Sargent M, et al. Diversity of the human intestinal microbial flora. Science 2005; 308:1635-1638; PMID:15831718; http://dx.doi.org/10.1126/science.1110591.
9. Goodman AL, Kallstrom G, Faith JJ, Reyes A, Moore A, Dantas G, et al. Extensive personal human gut microbiota culture collections characterized and manipulated in gnotobiotic mice. Proc Natl Acad Sci USA 2011; 108:6252-6257. PMID:21436049; http://dx.doi.org/10.1073/pnas.1102938108.
10. Tjellström B, Stenhammar L, Högberg L, Fälth-Magnusson K, Magnusson KE, Midtvedt T, et al. Gut microflora associated characteristics in firstdegree relatives of children with celiac disease. Scand J Gastroenterol 2007; 42:1204-1208. PMID:17852880; http://dx.doi.org/10.1080/00365520701320687.
11. Cardona ME. Effect of probiotics on five biochemical microflora-associated characteristics, in vitro and in vivo. Food & Nutrition Research 2002; 46:73-79.
12. Böttcher MF, Nordin EK, Sandin A, Midtvedt T, Björkstén B. Microflora-associated characteristics in faeces from allergic and nonallergic infants. Clin Exp Allergy 2000; 30:1590-1596. PMID:11069568; http://dx.doi.org/10.1046/j.1365-2222.2000.00982.x.
13. Bennet R, Eriksson M, Nord CE. The fecal microflora of 1-3-month-old infants during treatment with eight oral antibiotics. Infection 2002; 30:158-160. PMID:12120942; http://dx.doi.org/10.1007/s15010-002-2140-z.
14. Hascoët JM, Hubert C, Rochat F, Legagneur H, Gaga S, Emady-Azar S, et al. Effect of formula composition on the development of infant gut microbiota. J Pediatr Gastroenterol Nutr 2011; 52:756-762; PMID:21593648; http://dx.doi.org/10.1097/MPG.0b013e3182105850.
15. Fallani M, Amarri S, Uusijarvi A, Adam R, Khanna S, Aguilera M, et al.; INFABIO team. Determinants of the human infant intestinal microbiota after the introduction of first complementary foods in infant samples from five European centres. Microbiology 2011; 157:1385-1392. PMID:21330436; http://dx.doi.org/10.1099/mic.0.042143-0.
16. De Filippo C, Cavalieri D, Di Paola M, Ramazzotti M, Poullet JB, Massart S, et al. Impact of diet in shaping gut microbiota revealed by a comparative study in children from Europe and rural Africa. Proc Natl Acad Sci USA 2010; 107:14691-14696. PMID:20679230; http://dx.doi.org/10.1073/pnas.1005963107.
17. Midtvedt T, Bjørneklett A, Carlstedt-Duke B, Gustafsson BE, Høverstad T, Lingaas E, et al. The influence of antibiotics upon microflora-associated characteristics in man and mammals. Prog Clin Biol Res 1985; 181:241-244. PMID:4022981.
18. Tjellström B, Stenhammar L, Högberg L, Fälth-Magnusson K, Magnusson KE, Midtvedt T, et al. Gut microflora associated characteristics in children with celiac disease. Am J Gastroenterol 2005; 100:2784-788. PMID:16393236; http://dx.doi.org/10.1111/j.1572-0241.2005.00313.x.
19. Bezirtzoglou E, Stavropoulou E. Immunology and probiotic impact of the newborn and young children intestinal microflora. Anaerobe 2011; 17:369-3674. PMID:21515397; http://dx.doi.org/10.1016/j.anaerobe.2011.03.010.
20. Thompson-Chagoyan OC, Fallani M, Maldonado J, Vieites JM, Khanna S, Edwards C, et al. Faecal microbiota and short-chain fatty acid levels in faeces from infants with cow's milk protein allergy. Int Arch Allergy Immunol 2011; 156:325-332. PMID:21720179; http://dx.doi.org/10.1159/000323893.
21. Cani PD, Bibiloni R, Knauf C, Waget A, Neyrinck AM, Delzenne NM, et al. Changes in gut microbiota control metabolic endotoxemia-induced inflammation in high-fat diet-induced obesity and diabetes in mice. Diabetes 2008; 57:1470-1481. PMID:18305141; http://dx.doi.org/10.2337/db07-1403.
22. O'Toole PW, Claesson MJ. Gut microbiota: Changes throughout the lifespan from infancy to elderly. Int Dairy J 2010; 20:281-291. http://dx.doi.org/10.1016/j.idairyj.2009.11.010.
23. Malinen E, Kassinen A, Rinttilä T, Palva A. Comparison of real-time PCR with SYBR Green I or 5'-nuclease assays and dot-blot hybridization with rDNAtargeted oligonucleotide probes in quantification of selected faecal bacteria. Microbiology 2003; 149:269-277. PMID:12576600; http://dx.doi.org/10.1099/mic.0.25975-0.
24. Duncan SH, Lobley GE, Holtrop G, Ince J, Johnstone AM, Louis P, et al. Human colonic microbiota associated with diet, obesity and weight loss. Int J Obes (Lond) 2008; 32:1720-1724. PMID:18779823; http://dx.doi.org/10.1038/ijo.2008.155.
25. Manichanh C, Rigottier-Gois L, Bonnaud E, Gloux K, Pelletier E, Frangeul L, et al. Reduced diversity of faecal microbiota in Crohn's disease revealed by a metagenomic approach. Gut 2006; 55:205-211. PMID:16188921; http://dx.doi.org/10.1136/gut.2005.073817.
26. Overturf K. Quantitative PCR. Molecular Research in Aquaculture 2009; 39-61.
27. Zwielehner J, Liszt K, Handschur M, Lassl C, Lapin A, Haslberger AG. Combined PCR-DGGE fingerprinting and quantitative-PCR indicates shifts in fecal population sizes and diversity of Bacteroides, bifidobacteria and Clostridium cluster IV in institutionalized elderly. Exp Gerontol 2009; 44:440-446. PMID:19376217; http://dx.doi.org/10.1016/j.exger.2009.04.002.
28. Collado MC, Isolauri E, Laitinen K, Salminen S. Effect of mother's weight on infant's microbiota acquisition, composition and activity during early infancy: a prospective follow-up study initiated in early pregnancy. Am J Clin Nutr 2010; 92:1023-1030. PMID:20844065; http://dx.doi.org/10.3945/ajcn.2010.29877.
29. Brown PO, Botstein D. Exploring the new world of the genome with DNA microarrays. Nat Genet 1999; 21:33-37. PMID:9915498; http://dx.doi.org/10.1038/4462.
30. Frank DN, Pace NR. Gastrointestinal microbiology enters the metagenomics era. Curr Opin Gastroenterol 2008; 24:4-10. PMID:18043225; http://dx.doi.org/10.1097/MOG.0b013e3282f2b0e8.
31. Mardis ER. Next-generation DNA sequencing methods. Annu Rev Genomics Hum Genet 2008; 9:387-402. PMID:18576944; http://dx.doi.org/10.1146/annurev.genom.9.081307.164359.
32. Strausberg RL, Levy S, Rogers YH. Emerging DNA sequencing technologies for human genomic medicine. Drug Discov Today 2008; 13:569-577. PMID:18598911; http://dx.doi.org/10.1016/j.drudis.2008.03.025.
33. Mardis ER. The impact of next-generation sequencing technology on genetics. Trends Genet 2008; 24:133-141; PMID:18262675; http://dx.doi.org/10.1016/j.tig.2007.12.007.
34. Weber JL, Myers EW. Human whole-genome shotgun sequencing. Genome Res 1997; 7:401-409. PMID:9149936.
35. Hattori M, Taylor TD. The human intestinal microbiome: a new frontier of human biology. DNA Res 2009; 16:1-12. PMID:19147530; http://dx.doi.org/10.1093/dnares/dsn033.
36. Kurokawa K, Itoh T, Kuwahara T, Oshima K, Toh H, Toyoda A, et al. Comparative metagenomics revealed commonly enriched gene sets in human gut microbiomes. DNA Res 2007; 14:169-81. PMID:17916580; http://dx.doi.org/10.1093/dnares/dsm018.
37. Venter JC, Adams MD, Myers EW, Li PW, Mural RJ, Sutton GG, et al. The sequence of the human genome. Science 2001; 291:1304-1351. PMID:11181995; http://dx.doi.org/10.1126/science.1058040.
38. Turnbaugh PJ, Hamady M, Yatsunenko T, Cantarel BL, Duncan A, Ley RE, et al. A core gut microbiome in obese and lean twins. Nature 2009; 457:480-484. PMID:19043404; http://dx.doi.org/10.1038/nature07540.
39. Turnbaugh PJ, Ridaura VK, Faith JJ, Rey FE, Knight R, Gordon JI. The effect of diet on the human gut microbiome: a metagenomic analysis in humanized gnotobiotic mice. Sci Transl Med 2009; 1:6. PMID:20368178; http://dx.doi.org/10.1126/scitranslmed.3000322.
40. Claesson MJ, Cusack S, O'Sullivan O, Greene-Diniz R, de Weerd H, Flannery E, et al. Composition, variability and temporal stability of the intestinal microbiota of the elderly. Proc Natl Acad Sci USA 2011; 108:4586-4591. PMID:20571116; http://dx.doi.org/10.1073/pnas.1000097107.
41. Schadt EE, Turner S, Kasarskis A. A window into thirdgeneration sequencing. Hum Mol Genet 2010; 19:227-240. PMID:20858600; http://dx.doi.org/10.1093/hmg/ddq416.
42. Shendure J, Porreca GJ, Reppas NB, Lin X, McCutcheon JP, Rosenbaum AM, et al. Accurate multiplex polony sequencing of an evolved bacterial genome. Science 2005; 309:1728-1732. PMID:16081699; http://dx.doi.org/10.1126/science.1117389.
43. Levene MJ, Korlach J, Turner SW, Foquet M, Craighead HG, Webb WW. Zero-mode waveguides for single-molecule analysis at high concentrations. Science 2003; 299:682-686. PMID:12560545; http://dx.doi.org/10.1126/science.1079700.
44. Kasianowicz JJ, Brandin E, Branton D, Deamer DW. Characterization of individual polynucleotide molecules using a membrane channel. Proc Natl Acad Sci USA 1996; 93:13770-13773. PMID:8943010; http://dx.doi.org/10.1073/pnas.93.24.13770.
45. Clarke J, Wu HC, Jayasinghe L, Patel A, Reid S, Bayley H. Continuous base identification for single-molecule nanopore DNA sequencing. Nat Nanotechnol 2009; 4:265-270. PMID:19350039; http://dx.doi.org/10.1038/nnano.2009.12.
46. Roesch LFW, Lorca GL, Casella G, Giongo A, Naranjo A, Pionzio AM, et al. Culture-independent identification of gut bacteria correlated with the onset of diabetes in a rat model. ISME J 2009; 3:536-548. PMID:19225551; http://dx.doi.org/10.1038/ismej.2009.5.
47. Gophna U, Sommerfeld K, Gophna S, Doolittle WF, Veldhuyzen van Zanten SJO. Differences between tissue-associated intestinal microfloras of patients withCrohn's disease and ulcerative colitis. J Clin Microbiol 2006; 44:4136-4141. PMID:16988016; http://dx.doi.org/10.1128/JCM.01004-06.
48. Kassinen A, Krogius-Kurikka L, Mäkivuokko H, Rinttilä T, Paulin L, Corander J, et al. The fecal microbiota of irritable bowel syndrome patients differs significantly from that of healthy subjects. Gastroenterology 2007; 133:24-33. PMID:17631127; http://dx.doi.org/10.1053/j.gastro.2007.04.005.
49. Maitra A, Cohen Y, Gillespie SED, Mambo E, Fukushima N, Hoque MO, et al. The Human MitoChip: a high-throughput sequencing microarray for mitochondrial mutation detection. Genome Res 2004; 14:812-819. PMID:15123581; http://dx.doi.org/10.1101/gr.2228504.
50. Thomas RK, Baker AC, Debiasi RM, Winckler W, Laframboise T, Lin WM, et al. High-throughput oncogene mutation profiling in human cancer. Nat Genet 2007; 39:347-351. PMID:17293865; http://dx.doi.org/10.1038/ng1975.
51. Ley RE. Obesity and the human microbiome. Curr Opin Gastroenterol 2010; 26:5-11. PMID:19901833; http://dx.doi.org/10.1097/MOG.0b013e328333d751.
52. Ley RE, Bäckhed F, Turnbaugh P, Lozupone CA, Knight RD, Gordon JI. Obesity alters gut microbial ecology. Proc Natl Acad Sci USA 2005; 102:11070-11075. PMID:16033867; http://dx.doi.org/10.1073/pnas.0504978102.
53. Dethlefsen L, Huse S, Sogin ML, Relman DA. The pervasive effects of an antibiotic on the human gut microbiota, as revealed by deep 16S rRNA sequencing. PLoS Biol 2008; 6:280. PMID:19018661.
54. Grönlund MM, Lehtonen OP, Eerola E, Kero P. Fecal microflora in healthy infants born by different methods of delivery: permanent changes in intestinal flora after cesarean delivery. J Pediatr Gastroenterol Nutr 1999; 28:19-25. PMID:9890463; http://dx.doi.org/10.1097/00005176-199901000-00007.
55. Edwards CA, Parrett AM. Intestinal flora during the first months of life: new perspectives. Br J Nutr 2002; 88:11-18. PMID:12117423; http://dx.doi.org/10.1079/BJN2002625.
56. Salminen S, Gibson GR, McCartney AL, Isolauri E. Influence of mode of delivery on gut microbiota composition in seven year old children. Gut 2004; 53:1388-1389. PMID:15306608; http://dx.doi.org/10.1136/gut.2004.041640.
57. Stark PL, Lee A. The microbial ecology of the large bowel of breast-fed and formula-fed infants during the first year of life. J Med Microbiol 1982; 15:189-203. PMID:7143428; http://dx.doi.org/10.1099/00222615-15-2-189.
58. Mshvildadze M, Neu J. The infant intestinal microbiome: friend or foe? Early Hum Dev 2010; 86:67-71. PMID:20116944; http://dx.doi.org/10.1016/j.earlhumdev.2010.01.018.
59. Dominguez-Bello MG, Costello EK, Contreras M, Magris M, Hidalgo G, Fierer N, et al. Delivery mode shapes the acquisition and structure of the initial microbiota across multiple body habitats in newborns. Proc Natl Acad Sci USA 2010; 107:11971-1975. PMID:20566857; http://dx.doi.org/10.1073/pnas.1002601107.
60. Penders J, Thijs C, Vink C, Stelma FF, Snijders B, Kummeling I, et al. Factors influencing the composition of the intestinal microbiota in early infancy. Pediatrics 2006; 118:511-521. PMID:16882802; http://dx.doi.org/10.1542/peds.2005-824.
61. Fitzpatrick F, Oza A, Gilleece A, O'Byrne AM, Drudy D C. difficile subcommittee of the Health Protection Surveillance Centre. Laboratory diagnosis of Clostridium difficile-associated disease in the Republic of Ireland: a survey of Irish microbiology laboratories. J Hosp Infect 2008; 68:315-321. PMID:18353502; http://dx.doi.org/10.1016/j.jhin.2008.01.025.
62. Gerding DN, Johnson S, Peterson LR, Mulligan ME, Silva J Jr. Clostridium difficile-associated diarrhea and colitis. Infect Control Hosp Epidemiol 1995; 16:459-4577. PMID:7594392; http://dx.doi.org/10.1086/648363.
63. Thomas C, Stevenson M, Riley TV. Antibiotics and hospital-acquired Clostridium difficile-associated diarrhoea: a systematic review. J Antimicrob Chemother 2003; 51:1339-1350. PMID:12746372; http://dx.doi.org/10.1093/jac/dkg254.
64. van Nimwegen FA, Penders J, Stobberingh EE, Postma DS, Koppelman GH, Kerkhof M, et al. Mode and place of delivery, gastrointestinal microbiota, and their influence on asthma and atopy. J Allergy Clin Immunol 2011; 128:948-955. PMID:21872915; http://dx.doi.org/10.1016/j.jaci.2011.07.027.
65. Biasucci G, Rubini M, Riboni S, Morelli L, Bessi E, Retetangos C. Mode of delivery affects the bacterial community in the newborn gut. Early Hum Dev 2010; 86:13-15. PMID:20133091; http://dx.doi.org/10.1016/j.earlhumdev.2010.01.004.
66. Adlerberth I, Strachan DP, Matricardi PM, Ahrné S, Orfei L, Åberg N, et al. Gut microbiota and development of atopic eczema in 3 European birth cohorts. J Allergy Clin Immunol 2007; 120:343-350. PMID:17604093; http://dx.doi.org/10.1016/j.jaci.2007.05.018.
67. Huurre A, Kalliomäki M, Rautava S, Rinne M, Salminen S, Isolauri E. Mode of delivery-effects on gut microbiota and humoral immunity. Neonatology 2008; 93:236-420. PMID:18025796; http://dx.doi.org/10.1159/000111102.
68. Leahy SC, Higgins DG, Fitzgerald GF, van Sinderen D. Getting better with bifidobacteria. J Appl Microbiol 2005; 98:1303-1315. PMID:15916644; http://dx.doi.org/10.1111/j.1365-2672.2005.02600.x.
69. Picard C, Fioramonti J, Francois A, Robinson T, Neant F, Matuchansky C. Review article: bifidobacteria as probiotic agents-physiological effects and clinical benefits. Aliment Pharmacol Ther 2005; 22:495-512. PMID:16167966; http://dx.doi.org/10.1111/j.1365-2036.2005.02615.x.
70. Liepke C, Adermann K, Raida M, Mägert HJ, Forssmann WG, Zucht HD. Human milk provides peptides highly stimulating the growth of bifidobacteria. Eur J Biochem 2002; 269:712-718. PMID:11856332; http://dx.doi.org/10.1046/j.0014-2956.2001.02712.x.
71. EFSA. Opinion of the Scientific Committee on a request from EFSA on the introduction of a Qualified Presumption of Safety (QPS) approach for assessment of selected microorganisms referred to EFSA. The EFSA Journal 2007; 587:1-16.
72. Goldani HAS, Bettiol H, Barbieri MA, Silva AAM, Agranonik M, Morais MB, et al. Cesarean delivery is associated with an increased risk of obesity in adulthood in a Brazilian birth cohort study. Am J Clin Nutr 2011; 93:1344-1347. PMID:21508088; http://dx.doi.org/10.3945/ajcn.110.010033.
73. Neu J, Rushing J. Cesarean versus vaginal delivery: long-term infant outcomes and the hygiene hypothesis. Clin Perinatol 2011; 38:321-331. PMID:21645799; http://dx.doi.org/10.1016/j.clp.2011.03.008.
74. WHO. Global strategy on infant and young child feeding. Available online at (www.who.int/nutrition/publications/infantfeeding) 2003.
75. Kristiansen AL, Lande B, Øverby NC, Andersen LF. Factors associated with exclusive breast-feeding and breast-feeding in Norway. Public Health Nutr 2010; 13:2087-2096. PMID:20707948; http://dx.doi.org/10.1017/S1368980010002156.
76. Tarrant RC, Younger KM, Sheridan-Pereira M, White MJ, Kearney JM. The prevalence and determinants of breast-feeding initiation and duration in a sample of women in Ireland. Public Health Nutr 2010; 13:760-770. PMID:19758484; http://dx.doi.org/10.1017/S1368980009991522.
77. Survey NI. Breastfeeding Among US Children Born 1999-2007. CDC National Immunization Survey 2007.
78. Allen J, Hector D. Benefits of breastfeeding. NSW Public Health Bull 2005; 16:42-46. PMID:16106271; http://dx.doi.org/10.1071/NB05011.
79. Fox PF, Kelly AL. Indigenous enzymes in milk: Overview and historical aspects-Part 2. Int Dairy J 2006; 16:517-532. http://dx.doi.org/10.1016/j.idairyj.2005.09.017.
80. Manning TS, Gibson GR. Microbial-gut interactions in health and disease. Prebiotics. Best Pract Res Clin Gastroenterol 2004; 18:287-298. PMID:15123070; http://dx.doi.org/10.1016/j.bpg.2003.10.008.
81. Bezirtzoglou E, Tsiotsias A, Welling GW. Microbiota profile in feces of breast- and formula-fed newborns by using fluorescence in situ hybridization (FISH). Anaerobe 2011; 17:478-482. PMID:21497661; http://dx.doi.org/10.1016/j.anaerobe.2011.03.009.
82. Fallani M, Young D, Scott J, Norin E, Amarri S, Adam R, et al.; and Other Members of the INFABIO Team. Intestinal microbiota of 6-week-old infants across Europe: geographic influence beyond delivery mode, breast-feeding and antibiotics. J Pediatr Gastroenterol Nutr 2010; 51:77-84. PMID:20479681; http://dx.doi.org/10.1097/MPG.0b013e3181d1b11e.
83. Le Huërou-Luron I, Blat S, Boudry G. Breast- v. formula-feeding: impacts on the digestive tract and immediate and long-term health effects. Nutr Res Rev 2010; 23:23-36. PMID:20450531; http://dx.doi.org/10.1017/S0954422410000065.
84. Roger LC, Costabile A, Holland DT, Hoyles L, McCartney AL. Examination of faecal Bifidobacterium populations in breast- and formula-fed infants during the first 18 months of life. Microbiology 2010; 156:3329-33241. PMID:20864478; http://dx.doi.org/10.1099/mic.0.043224-0.
85. Lin PW, Stoll BJ. Necrotising enterocolitis. Lancet 2006; 368:1271-1283. PMID:17027734; http://dx.doi.org/10.1016/S0140-6736(06)69525-1.
86. Martín R, Langa S, Reviriego C, Jimínez E, Marín ML, Xaus J, et al. Human milk is a source of lactic acid bacteria for the infant gut. J Pediatr 2003; 143:754-758. PMID:14657823; http://dx.doi.org/10.1016/j.jpeds.2003.09.028.
87. Martín R, Heilig GHJ, Zoetendal EG, Smidt H, Rodríguez JM. Diversity of the Lactobacillus group in breast milk and vagina of healthy women and potential role in the colonization of the infant gut. J Appl Microbiol 2007; 103:2638-644. PMID:18045446; http://dx.doi.org/10.1111/j.1365-2672.2007.03497.x.
88. Sinkiewicz G, Ljunggren L. Occurrence of Lactobacillus reuteri in human breast milk. Microb Ecol Health Dis 2008; 20:122-126. http://dx.doi.org/10.1080/08910600802341007.
89. Solís G, de Los Reyes-Gavilan CG, Fernández N, Margolles A, Gueimonde M. Establishment and development of lactic acid bacteria and bifidobacteria microbiota in breast-milk and the infant gut. Anaerobe 2010; 16:307-310. PMID:20176122; http://dx.doi.org/10.1016/j.anaerobe.2010.02.004.
90. Mohan P, Abrams SA. Oral lactoferrin for the treatment of sepsis and necrotizing enterocolitis in neonates. Cochrane Database Syst Rev 2009; 7138. PMID:19160324.
91. Agostoni C, Decsi T, Fewtrell M, Goulet O, Kolacek S, Koletzko B, et al.; ESPGHAN Committee on Nutrition. Complementary feeding: a commentary by the ESPGHAN Committee on Nutrition. J Pediatr Gastroenterol Nutr 2008; 46:99-110. PMID:18162844; http://dx.doi.org/10.1097/01.mpg.0000304464.60788.bd.
92. Koenig JE, Spor A, Scalfone N, Fricker AD, Stombaugh J, Knight R, et al. Succession of microbial consortia in the developing infant gut microbiome. Proc Natl Acad Sci USA 2011; 108:4578-4585. PMID:20668239; http://dx.doi.org/10.1073/pnas.1000081107.
93. Strachan DP. Hay fever, hygiene and household size. BMJ 1989; 299:1259-1260. PMID:2513902; http://dx.doi.org/10.1136/bmj.299.6710.1259.
94. McKeever TM, Lewis SA, Smith C, Hubbard R. The importance of prenatal exposures on the development of allergic disease: a birth cohort study using the West Midlands General Practice Database. Am J Respir Crit Care Med 2002; 166:827-832. PMID:12231492; http://dx.doi.org/10.1164/rccm.200202-158OC.
95. Strachan DP. Family size, infection and atopy: the first decade of the 'hygiene hypothesis'. Thorax 2000; 55:2-10. http://dx.doi.org/10.1136/thorax.55.suppl_1.S2.
96. WHO. WHO obesity factsheet. Available online at (http://www.who.int/mediacentre/factsheets/fs311/en/index.html) 2011.
97. Lawlor DA, Smith GD, O'Callaghan M, Alati R, Mamun AA, Williams GM, et al. Epidemiologic evidence for the fetal overnutrition hypothesis: findings from the mater-university study of pregnancy and its outcomes. Am J Epidemiol 2007; 165:418-424. PMID:17158475; http://dx.doi.org/10.1093/aje/kwk030.
98. Kalliomäki M, Collado MC, Salminen S, Isolauri E. Early differences in fecal microbiota composition in children may predict overweight. Am J Clin Nutr 2008; 87:534-538. PMID:18326589.
99. Gueimonde M, Sakata S, Kalliomäki M, Isolauri E, Benno Y, Salminen S. Effect of maternal consumption of lactobacillus GG on transfer and establishment of fecal bifidobacterial microbiota in neonates. J Pediatr Gastroenterol Nutr 2006; 42:166-170. PMID:16456409; http://dx.doi.org/10.1097/01.mpg.0000189346.25172.fd.
100. Mangin I, Suau A, Gotteland M, Brunser O, Pochart P. Amoxicillin treatment modifies the composition of Bifidobacterium species in infant intestinal microbiota. Anaerobe 2010; 16:433-438. PMID:20601031; http://dx.doi.org/10.1016/j.anaerobe.2010.06.005.
101. Parker RB. Probiotics, the other half of the antibiotic story. Animal Nutrition and Health 1974; 29:4-8.
102. Fuller R. Probiotics in man and animals. J Appl Bacteriol 1989; 66:365-378. PMID:2666378; http://dx.doi.org/10.1111/j.1365-2672.1989.tb05105.x.
103. Salminen S, Ouwehand A, Benno Y, Lee YK. Probiotics: how should they be defined? Trends Food Sci Technol 1999; 10:107-1010. http://dx.doi.org/10.1016/S0924-2244(99)00027-8.
104. Lilly DM, Stillwell RH. Probiotics: growth-promoting factors produced by microorganisms. Science 1965; 147:747-748. PMID:14242024; http://dx.doi.org/10.1126/science.147.3659.747.
105. FAO/WHO. Health and nutritional properties of probiotics in food including powder milk with live lactic acid bacteria. Report of a Joint FAO/WHO Expert Consultation on Evaluation of Health and Nutritional Properties of Probiotics in Foods Including Powder Milk with Live Lactic Acid Bacteria. Basel, Switzerland: World Health Organization 2001.
106. Parracho H, McCartney AL, Gibson GR. Probiotics and prebiotics in infant nutrition. Proc Nutr Soc 2007; 66:405-411. PMID:17637093; http://dx.doi.org/10.1017/S0029665107005678.
107. Holzapfel WH, Schillinger U. Introduction to pre-and probiotics. Food Res Int 2002; 35:109-116. http://dx.doi.org/10.1016/S0963-9969(01)00171-5.
108. EFSA. EFSA on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on on the scientific requirements for health claims related to gut and immune function. Available online at (www.efsa.europa.eu/efsajournal.html) 2010.
109. Aureli P, Capurso L, Castellazzi AM, Clerici M, Giovannini M, Morelli L, et al. Probiotics and health: an evidence-based review. Pharmacol Res 2011; 63:366-376; PMID:21349334; http://dx.doi.org/10.1016/j.phrs.2011.02.006.
110. Preidis GA, Versalovic J. Targeting the human microbiome with antibiotics, probiotics and prebiotics: gastroenterology enters the metagenomics era. Gastroenterology 2009; 136:2015-2031. PMID:19462507; http://dx.doi.org/10.1053/j.gastro.2009.01.072.
111. Rastall RA, Gibson GR, Gill HS, Guarner F, Klaenhammer TR, Pot B, et al. Modulation of the microbial ecology of the human colon by probiotics, prebiotics and synbiotics to enhance human health: an overview of enabling science and potential applications. FEMS Microbiol Ecol 2005; 52:145-152. PMID:16329901; http://dx.doi.org/10.1016/j.femsec.2005.01.003.
112. Wallace TC, Guarner F, Madsen K, Cabana MD, Gibson G, Hentges E, et al. Human gut microbiota and its relationship to health and disease. Nutr Rev 2011; 69:392-403. PMID:21729093; http://dx.doi.org/10.1111/j.1753-4887.2011.00402.x.
113. Kitajima H, Sumida Y, Tanaka R, Yuki N, Takayama H, Fujimura M. Early administration of Bifidobacterium breve to preterm infants: randomised controlled trial. Arch Dis Child Fetal Neonatal Ed 1997; 76:101-107. http://dx.doi.org/10.1136/fn.76.2.F101.
114. Grzeskowiak Ł, Grönlund MM, Beckmann C, Salminen S, von Berg A, Isolauri E. The impact of perinatal probiotic intervention on gut microbiota: doubleblind placebo-controlled trials in Finland and Germany. Anaerobe 2012; 18:7-13. http://dx.doi.org/10.1016/j.anaerobe.2011.09.006; PMID:21979491.
115. Gerrard JW, Geddes CA, Reggin PL, Gerrard CD, Horne S. Serum IgE levels in white and metis communities in Saskatchewan. Ann Allergy 1976; 37:91-100. PMID:987744.
116. Sepp E, Julge K, Vasar M, Naaber P, Björksten B, Mikelsaar M. Intestinal microflora of Estonian and Swedish infants. Acta Paediatr 1997; 86:956-961. PMID:9343275; http://dx.doi.org/10.1111/j.1651-2227.1997.tb15178.x.
117. Bourlioux P, Koletzko B, Guarner F, Braesco V. The intestine and its microflora are partners for the protection of the host: report on the Danone Symposium "The Intelligent Intestine", held in Paris, June 14, 2002. Am J Clin Nutr 2003; 78:675-683. PMID:14522724.
118. Saavedra JM. Use of probiotics in pediatrics: rationale, mechanisms of action and practical aspects. Nutr Clin Pract 2007; 22:351-365. PMID:17507735; http://dx.doi.org/10.1177/0115426507022003351.
119. Johansson MA, Sjögren YM, Persson JO, Nilsson C, Sverremark-Ekström E. Early colonization with a group of Lactobacilli decreases the risk for allergy at five years of age despite allergic heredity. PLoS One 2011; 6:23031. PMID:21829685; http://dx.doi.org/10.1371/journal.pone.0023031.
120. Bach JF. The effect of infections on susceptibility to autoimmune and allergic diseases. N Engl J Med 2002; 347:911-920. PMID:12239261; http://dx.doi.org/10.1056/NEJMra020100.
121. Rautava S, Kalliomäki M, Isolauri E. Probiotics during pregnancy and breast-feeding might confer immunomodulatory protection against atopic disease in the infant. J Allergy Clin Immunol 2002; 109:119-121. PMID:11799376; http://dx.doi.org/10.1067/mai.2002.120273.
122. Kukkonen K, Savilahti E, Haahtela T, Juntunen-Backman K, Korpela R, Poussa T, et al. Probiotics and prebiotic galacto-oligosaccharides in the prevention of allergic diseases: a randomized, double-blind, placebo-controlled trial. J Allergy Clin Immunol 2007; 119:192-198. PMID:17208601; http://dx.doi.org/10.1016/j.jaci.2006.09.009.
123. Viljanen M, Savilahti E, Haahtela T, Juntunen-Backman K, Korpela R, Poussa T, et al. Probiotics in the treatment of atopic eczema/dermatitis syndrome in infants: a double-blind placebo-controlled trial. Allergy 2005; 60:494-500. PMID:15727582; http://dx.doi.org/10.1111/j.1398-9995.2004.00514.x.
124. Prescott SL, Björkstén B. Probiotics for the prevention or treatment of allergic diseases. J Allergy Clin Immunol 2007; 120:255-262. PMID:17544096; http://dx.doi.org/10.1016/j.jaci.2007.04.027.
125. Boyle RJ, Bath-Hextall FJ, Leonardi-Bee J, Murrell DF, Tang ML. Probiotics for the treatment of eczema: a systematic review. Clin Exp Allergy 2009; 39:1117-1127. PMID:19573037; http://dx.doi.org/10.1111/j.1365-2222.2009.03305.x.
126. Kalliomäki M, Antoine JM, Herz U, Rijkers GT, Wells JM, Mercenier A. Guidance for substantiating the evidence for beneficial effects of probiotics: prevention and management of allergic diseases by probiotics. J Nutr 2010; 140:713-721. PMID:20130079; http://dx.doi.org/10.3945/jn.109.113761.
127. Wang M, Karlsson C, Olsson C, Adlerberth I, Wold AE, Strachan DP, et al. Reduced diversity in the early fecal microbiota of infants with atopic eczema. J Allergy Clin Immunol 2008; 121:129-134. PMID:18028995; http://dx.doi.org/10.1016/j.jaci.2007.09.011.
128. Jernberg C, Löfmark S, Edlund C, Jansson JK. Longterm impacts of antibiotic exposure on the human intestinal microbiota. Microbiology 2010; 156:3216-3223. PMID:20705661; http://dx.doi.org/10.1099/mic.0.040618-0.
129. Jernberg C, Löfmark S, Edlund C, Jansson JK. Longterm ecological impacts of antibiotic administration on the human intestinal microbiota. ISME J 2007; 1:56-66. PMID:18043614; http://dx.doi.org/10.1038/ismej.2007.3.
130. Claud EC, Walker WA. Hypothesis: inappropriate colonization of the premature intestine can cause neonatal necrotizing enterocolitis. FASEB J 2001; 15:1398-1403. PMID:11387237; http://dx.doi.org/10.1096/fj.00-0833hyp.
131. Deshpande G, Rao S, Patole S. Probiotics for prevention of necrotising enterocolitis in preterm neonates with very low birthweight: a systematic review of randomized controlled trials. Lancet 2007; 369:1614-1620. PMID:17499603; http://dx.doi.org/10.1016/S0140-6736(07)60748-X.
132. Braga TD, da Silva GAP, de Lira PIC, de Carvalho Lima M. Efficacy of Bifidobacterium breve and Lactobacillus casei oral supplementation on necrotizing enterocolitis in very-low-birth-weight preterm infants: a double-blind, randomized, controlled trial. Am J Clin Nutr 2011; 93:81-86. PMID:20980486; http://dx.doi.org/10.3945/ajcn.2010.29799.
133. Caplan MS, Lickerman M, Adler L, Dietsch GN, Yu A. The role of recombinant platelet-activating factor acetylhydrolase in a neonatal rat model of necrotizing enterocolitis. Pediatr Res 1997; 42:779-783. PMID:9396557; http://dx.doi.org/10.1203/00006450-199712000-00010.
134. Caplan MS, Miller-Catchpole R, Kaup S, Russell T, Lickerman M, Amer M, et al. Bifidobacterial supplementation reduces the incidence of necrotizing enterocolitis in a neonatal rat model. Gastroenterology 1999; 117:577-583. PMID:10464133; http://dx.doi.org/10.1016/S0016-5085(99)70450-6.
135. Lin HC, Su BH, Chen AC, Lin TW, Tsai CH, Yeh TF, et al. Oral probiotics reduce the incidence and severity of necrotizing enterocolitis in very low birth weight infants. Pediatrics 2005; 115:1-4. PMID:15629973.
136. Hoyos AB. Reduced incidence of necrotizing enterocolitis associated with enteral administration of Lactobacillus acidophilus and Bifidobacterium infantis to neonates in an intensive care unit. Int J Infect Dis 1999; 3:197-202. PMID:10575148; http://dx.doi.org/10.1016/S1201-9712(99)90024-3.
137. Bin-Nun A, Bromiker R, Wilschanski M, Kaplan M, Rudensky B, Caplan M, et al. Oral probiotics prevent necrotizing enterocolitis in very low birth weight neonates. J Pediatr 2005; 147:192-196. PMID:16126048; http://dx.doi.org/10.1016/j.jpeds.2005.03.054.
138. Alfaleh K, Anabrees J, Bassler D, Al-Kharfi T. Probiotics for prevention of necrotizing enterocolitis in preterm infants. Cochrane Database Syst Rev 2011; CD005496. http://dx.doi.org/10.1002/14651858.CD005496.pub3.
139. Barclay AR, Stenson B, Simpson JH, Weaver LT, Wilson DC. Probiotics for necrotizing enterocolitis: a systematic review. J Pediatr Gastroenterol Nutr 2007; 45:569-76. PMID:18030235; http://dx.doi.org/10.1097/MPG.0b013e3181344694.
140. Deshpande G, Rao S, Patole S, Bulsara M. Updated meta-analysis of probiotics for preventing necrotizing enterocolitis in preterm neonates. Pediatrics 2010; 125:921-930. PMID:20403939; http://dx.doi.org/10.1542/peds.2009-1301.
141. Gooch WM, 3rd, Blair E, Puopolo A, Paster RZ, Schwartz RH, Miller HC, et al. Effectiveness of five days of therapy with cefuroxime axetil suspension for treatment of acute otitis media. Pediatr Infect Dis J 1996; 15:157-164. PMID:8822290; http://dx.doi.org/10.1097/00006454-199602000-00013.
142. Hoberman A, Paradise JL, Burch DJ, Valinski WA, Hedrick JA, Aronovitz GH, et al. Equivalent efficacy and reduced occurrence of diarrhea from a new formulation of amoxicillin/clavulanate potassium (Augmentin) for treatment of acute otitis media in children. Pediatr Infect Dis J 1997; 16:463-470. PMID:9154538; http://dx.doi.org/10.1097/00006454-199705000-00002.
143. Cremonini F, Di Caro S, Nista EC, Bartolozzi F, Capelli G, Gasbarrini G, et al. Meta-analysis: the effect of probiotic administration on antibiotic-associated diarrhoea. Aliment Pharmacol Ther 2002; 16:1461-1467. PMID:12182746; http://dx.doi.org/10.1046/j.1365-2036.2002.01318.x.
144. D'Souza AL, Rajkumar C, Cooke J, Bulpitt CJ. Probiotics in prevention of antibiotic associated diarrhoea: meta-analysis. BMJ 2002; 324:1361-1367. PMID:12052801; http://dx.doi.org/10.1136/bmj.324.7350.1361.
145. Tankanow RM, Ross MB, Ertel IJ, Dickinson DG, McCormick LS, Garfinkel JF. A double-blind, placebocontrolled study of the efficacy of Lactinex in the prophylaxis of amoxicillin-induced diarrhea. DICP 1990; 24:382-384. PMID:2109432.
146. Agostoni C, Axelsson I, Braegger C, Goulet O, Koletzko B, Michaelsen KF, et al.; ESPGHAN Committee on Nutrition. Probiotic bacteria in dietetic products for infants: a commentary by the ESPGHAN Committee on Nutrition. J Pediatr Gastroenterol Nutr 2004; 38:365-374. PMID:15085012; http://dx.doi.org/10.1097/00005176-200404000-00001.
147. Segarra-Newnham M. Probiotics for Clostridium difficile-associated diarrhea: focus on Lactobacillus rhamnosus GG and Saccharomyces boulardii. Ann Pharmacother 2007; 41:1212-1221. PMID:17595306; http://dx.doi.org/10.1345/aph.1K110.
148. Gibson GR, Roberfroid MB. Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics. J Nutr 1995; 125:1401-1412. PMID:7782892.
149. Veereman-Wauters G. Application of prebiotics in infant foods. Br J Nutr 2005; 93:57-60. PMID:15877896; http://dx.doi.org/10.1079/BJN20041354.
150. Playne MJ, Crittenden R. Commercially available oligosaccharides. Bulletin-FIL-IDF (Belgium); International Dairy Federation 1996.
151. Wang X, Gibson GR. Effects of the in vitro fermentation of oligofructose and inulin by bacteria growing in the human large intestine. J Appl Bacteriol 1993; 75:373-380. PMID:8226394; http://dx.doi.org/10.1111/j.1365-2672.1993.tb02790.x.
152. Roberfroid M, Gibson GR, Hoyles L, McCartney AL, Rastall R, Rowland I, et al. Prebiotic effects: metabolic and health benefits. Br J Nutr 2010; 104:1-63. PMID:20920376; http://dx.doi.org/10.1017/S0007114510003363.
153. Reddy BS. Possible mechanisms by which pro- and prebiotics influence colon carcinogenesis and tumor growth. J Nutr 1999; 129:1478-1482. PMID:10395625.
154. Arslanoglu S, Moro GE, Boehm G. Early supplementation of prebiotic oligosaccharides protects formula-fed infants against infections during the first 6 months of life. J Nutr 2007; 137:2420-2424. PMID:17951479.
155. Gibson GR, Probert HM, Loo JV, Rastall RA, Roberfroid MB. Dietary modulation of the human colonic microbiota: updating the concept of prebiotics. Nutr Res Rev 2004; 17:259-275. PMID:19079930; http://dx.doi.org/10.1079/NRR200479.
156. Macfarlane S, Macfarlane GT, Cummings JH. Review article: prebiotics in the gastrointestinal tract. Aliment Pharmacol Ther 2006; 24:701-714. PMID:16918875; http://dx.doi.org/10.1111/j.1365-2036.2006.03042.x.
157. Agostoni C, Axelsson I, Goulet O, Koletzko B, Michaelsen KF, Puntis JWL, et al.; ESPGHAN Committee on Nutrition. Prebiotic oligosaccharides in dietetic products for infants: a commentary by the ESPGHAN Committee on Nutrition. J Pediatr Gastroenterol Nutr 2004; 39:465-473. PMID:15572882; http://dx.doi.org/10.1097/00005176-200411000-00003.
158. Ben XM, Zhou XY, Zhao WH, Yu WL, Pan W, Zhang WL, et al. Supplementation of milk formula with galacto-oligosaccharides improves intestinal micro-flora and fermentation in term infants. Chin Med J (Engl) 2004; 117:927-931. PMID:15198901.
159. Waligora-Dupriet AJ, Campeotto F, Nicolis I, Bonet A, Soulaines P, Dupont C, et al. Effect of oligofructose supplementation on gut microflora and well-being in young children attending a day care centre. Int J Food Microbiol 2007; 113:108-113. PMID:16996154; http://dx.doi.org/10.1016/j.ijfoodmicro.2006.07.009.
160. Boehm G, Moro G. Structural and functional aspects of prebiotics used in infant nutrition. J Nutr 2008; 138:1818-1828. PMID:18716193.
161. Howie PW, Forsyth JS, Ogston SA, Clark A, Florey CD. Protective effect of breast feeding against infection. BMJ 1990; 300:11-16. PMID:2105113; http://dx.doi.org/10.1136/bmj.300.6716.11.
162. Boehm G, Stahl B, Jelinek J, Knol J, Miniello V, Moro GE. Prebiotic carbohydrates in human milk and formulas. Acta Paediatr Suppl 2005; 94:18-21. PMID:16214760; http://dx.doi.org/10.1080/08035320510043493.
163. Haarman M, Knol J. Quantitative real-time PCR assays to identify and quantify fecal Bifidobacterium species in infants receiving a prebiotic infant formula. Appl Environ Microbiol 2005; 71:2318-2324. PMID:15870317; http://dx.doi.org/10.1128/AEM.71.5.2318-24.2005.
164. Rinne MM, Gueimonde M, Kalliomäki M, Hoppu U, Salminen SJ, Isolauri E. Similar bifidogenic effects of prebiotic-supplemented partially hydrolyzed infant formula and breastfeeding on infant gut microbiota. FEMS Immunol Med Microbiol 2005; 43:59-65. PMID:15607637; http://dx.doi.org/10.1016/j.femsim.2004.07.005.
165. Salvini F, Riva E, Salvatici E, Boehm G, Jelinek J, Banderali G, et al. A specific prebiotic mixture added to starting infant formula has long-lasting bifidogenic effects. J Nutr 2011; 141:1335-1339. PMID:21613452; http://dx.doi.org/10.3945/jn.110.136747.
166. Finegold SM. Interaction of antimicrobial therapy and intestinal flora. Am J Clin Nutr 1970; 23:1466-1471. PMID:4319890.
167. Sakata H, Yoshioka H, Fujita K. Development of the intestinal flora in very low birth weight infants compared to normal full-term newborns. Eur J Pediatr 1985; 144:186-190. PMID:4043132; http://dx.doi.org/10.1007/BF00451911.
168. Tanaka S, Kobayashi T, Songjinda P, Tateyama A, Tsubouchi M, Kiyohara C, et al. Influence of antibiotic exposure in the early postnatal period on the development of intestinal microbiota. FEMS Immunol Med Microbiol 2009; 56:80-87. PMID:19385995; http://dx.doi.org/10.1111/j.1574-695X.2009.00553.x.
169. Favier CF, de Vos WM, Akkermans ADL. Development of bacterial and bifidobacterial communities in feces of newborn babies. Anaerobe 2003; 9:219-229. PMID:16887708; http://dx.doi.org/10.1016/j.anaerobe.2003.07.001.
170. Hussey S, Wall R, Gruffman E, O'Sullivan L, Ryan CA, Murphy B, et al. Parenteral antibiotics reduce bifidobacteria colonization and diversity in neonates. Int J Microbiol 2011; 2011:130574. PMID:20811542; http://dx.doi.org/10.1155/2011/130574.
171. Ajslev TA, Andersen CS, Gamborg M, Sørensen TI, Jess T. Childhood overweight after establishment of the gut microbiota: the role of delivery mode, pre-pregnancy weight and early administration of antibiotics. Int J Obes (Lond) 2011; 35:522-529. PMID:21386800; http://dx.doi.org/10.1038/ijo.2011.27.
172. Jedrychowski W, Gałaś A, Whyatt R, Perera F. The prenatal use of antibiotics and the development of allergic disease in one year old infants. A preliminary study. Int J Occup Med Environ Health 2006; 19:70-76. PMID:16881601; http://dx.doi.org/10.2478/v10001-006-0010-0.
173. Foliaki S, Pearce N, Björkstén B, Mallol J, Montefort S, von Mutius E; International Study of Asthma and Allergies in Childhood Phase III Study Group. Antibiotic use in infancy and symptoms of asthma, rhinoconjunctivitis and eczema in children 6 and 7 years old: International Study of Asthma and Allergies in Childhood Phase III. J Allergy Clin Immunol 2009; 124:982-989. PMID:19895986; http://dx.doi.org/10.1016/j.jaci.2009.08.017.
174. Kozyrskyj AL, Ernst P, Becker AB. Increased risk of childhood asthma from antibiotic use in early life. Chest 2007; 131:1753-1759. PMID:17413050; http://dx.doi.org/10.1378/chest.06-3008.
175. Marra F, Marra CA, Richardson K, Lynd LD, Kozyrskyj A, Patrick DM, et al. Antibiotic use in children is associated with increased risk of asthma. Pediatrics 2009; 123:1003-1010. PMID:19255032; http://dx.doi.org/10.1542/peds.2008-1146.
176. Bedford Russell AR, Murch SH. Could peripartum antibiotics have delayed health consequences for the infant? BJOG 2006; 113:758-765. PMID:16827757; http://dx.doi.org/10.1111/j.1471-0528.2006.00952.x.
177. Penders J, Kummeling I, Thijs C. Infant antibiotic use and wheeze and asthma risk: a systematic review and meta-analysis. Eur Respir J 2011; 38:295-302. PMID:21233272; http://dx.doi.org/10.1183/09031936.00105010.
178. Celedón JC, Fuhlbrigge A, Rifas-Shiman S, Weiss ST, Finkelstein JA. Antibiotic use in the first year of life and asthma in early childhood. Clin Exp Allergy 2004; 34:1011-1016. PMID:15248843; http://dx.doi.org/10.1111/j.1365-2222.2004.01994.x.
179. Kummeling I, Stelma FF, Dagnelie PC, Snijders BEP, Penders J, Huber M, et al. Early life exposure to antibiotics and the subsequent development of eczema, wheeze and allergic sensitization in the first 2 years of life: the KOALA Birth Cohort Study. Pediatrics 2007; 119:225-231. PMID:17200248; http://dx.doi.org/10.1542/peds.2006-0896.
180. Abrahamsson TR, Jakobsson HE, Andersson AF, Björkstén B, Engstrand L, Jenmalm MC. Low diversity of the gut microbiota in infants with atopic eczema. J Allergy Clin Immunol 2012; 129:434-440. PMID:22153774; http://dx.doi.org/10.1016/j.jaci.2011.10.025.
181. Johnston, B., A. Supina, M. Ospina, and S. Vohra. 2007. Probiotics for the prevention of pediatric antibiotic-associated diarrhea. Cochrane Database Syst Rev 2007. PMID:17443557; http://dx.doi.org/10.1002/14651858.CD004827.pub2.