Zero-mode waveguides for single-molecule analysis at high concentrations

Science

Volumn 299, Issue 5607, 2003, Pages 682-686

  Levene, H J ^a   Korlach, J ^a,b   Turner, S W ^a,c   Foquet, M ^a   Craighead, H G ^a   Webb, W W ^a  

^a School of Operations Research and Industrial Engineering   (United States)

^b Nanofluidics Incorporated ^*  (United States)

^c Nanofluidics Incorporated   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; MOLECULAR DYNAMICS;

TEMPORAL RESOLUTION;

WAVEGUIDES;

DNA POLYMERASE; LIGAND;

BIOLOGY; DNA;

ARTICLE; BIOTECHNOLOGY; CONCENTRATION RESPONSE; DYNAMICS; ENZYME ACTIVITY; FILM; PRIORITY JOURNAL; WAVEFORM;

BIOCHEMISTRY; COMPUTER SIMULATION; COUMARINS; DEOXYCYTOSINE NUCLEOTIDES; DIFFUSION; DNA; DNA-DIRECTED DNA POLYMERASE; ENZYMES, IMMOBILIZED; FINITE ELEMENT ANALYSIS; FLUORESCENCE; FLUORESCENT DYES; LIGANDS; LIGHT; NANOTECHNOLOGY; OPTICS; SPECTROMETRY, FLUORESCENCE;

EID: 0037474152     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1079700     Document Type: Article

References (33)

1. X. S. Xie, H. P. Lu, J. Biol. Chem. 274, 15967 (1999).
2. H. P. Lu, L. Xun, X. S. Xie, Science 282, 1877 (1998).
3. A. Castro, J. G. Williams, Anal. Chem. 69, 3915 (1997).
4. R. A. Keller et al., Anal. Chem. 74, 316A (2002).
5. A. T. Woolley, C. Guillemette, C. Li Cheung, D. E. Housman, C. M. Lieber, Nature Biotechnol. 18, 760 (2000).
6. D. Magde, E. Elson, W. W. Webb, Phys. Rev. Lett. 29, 705 (1972).
7. J. Mertz, C. Xu, W. W. Webb, Optics Lett. 20, 2532 (1995).
8. L. 5. Barak, W. W. Webb, J. Cell Biol. 90, 595 (1981).
9. M. A. Medina, P. Schwille, Bioessays 24, 758 (2002).
10. F. J. Meyer-Almes, M. Auer, Biochemistry 39, 13261 (2000).
11. D. Schomburg, I. Schomburg, Springer Handbook of Enzymes (Springer, New York, ed. 2, 2001).
12. H. P. Lerch, A. S. Mikhailov, B. Hess, Proc. Natl. Acad. Sci. U.S.A. 99, 15410 (2002).
13. T. E. Starr, N. L Thompson, Biophys. J. 80, 1575 (2001).
14. F. de Lange et al., J. Cell Sci. 114, 4153 (2001).
15. E. Betzig, R. J. Chichester, Science 262, 1422 (1993).
16. D. R. Reyes, D. Iossifidis, P.-A. Auroux, A. Manz, Anal. Chem. 74, 2623 (2002).
17. P.-A. Auroux, D. Iossifidis, D. R. Reyes, A. Manz, Anal. Chem. 74, 2637 (2002).
18. H. G. Craighead, Science 290, 1532 (2000).
19. J. D. Jackson, Classical Electrodynamics (Wiley & Sons, New York, ed. 3, 1999).
20. K. Rippe, Biochemistry 39, 2131 (2000).
21. J. Ricka, T. Binkert, Phys. Rev. A 39, 2646 (1989).
22. Materials and methods are available as supporting material on Science Online.
23. D. Kleppner, Phys. Rev. Lett. 47, 233 (1981).
24. E. M. Purcell, Phys. Rev. 69, 681 (1946).
25. E. Yablonivitch, Phys. Rev. Lett. 58, 2059 (1987).
26. C. Xu, W. W. Webb, in Topics in Fluorescence Spectroscopy, vol. 5, Nonlinear and Two-Photon-Induced Fluorescence, J. Lakowicz, Ed. (Plenum Press, New York, 1997), pp. 471-540.
27. E. L. Elson, D. Magde, Biopolymers 13, 1 (1974).
28. N. L Thompson, T. P. Burghardt, D. Axelrod, Biophys. J. 33, 435 (1981).
29. D. E. Koppel, Phys. Rev. A 10, 1938 (1974).
30. Coumarin-dCTP was found to be efficiently and processively incorporated into DNA by Sequenase (USB Corporation, Cleveland, OH), a mutant T7 DNA polymerase. Detailed reaction conditions are given in (22).
31. S. S. Patel, I. Wong, K. A. Johnson, Biochemistry 30, 511 (1991).
32. J. Korlach et al., Patent Cooperation Treaty International Application No. WO 0070073, Cornell Research Foundation (2000).
33. We thank K. Hodgson for Fig. 1, M. Isaacson for obtaining Fig. 3D, and Amersham Biosciences for providing R110-dCTP. Supported by grant DE-FG02-99ER62809 from the U.S. Department of Energy. Additional support was received from NSF grant DBI-0080792 and from the facilities of National Center for Research Resources -NIH Biomedical Technology Resource, grant P41RR04224, and the Nanobiotechnology Center, NSF-Science and Technology Center grant agreement ECS-9876771. Device fabrication was performed at the Cornell Nanofabrication Facility.