Comparison of real-time PCR with SYBR green I or 5′-nuclease assays and dot-blot hybridization with rDNA-targeted oligonucleotide probes in quantification of selected faecal bacteria

Microbiology

Volumn 149, Issue 1, 2003, Pages 269-277

  Malinen, Erja ^a   Kassinen, Anna ^a   Rinttilä, Teemu ^a   Palva, Airi ^a  

^a UNIVERSITY OF HELSINKI   (Finland)

Author keywords

[No Author keywords available]

Indexed keywords

DNA POLYMERASE; DYE; INTERCALATING AGENT; NUCLEASE; OLIGONUCLEOTIDE; PHOSPHORUS; PRIMER DNA; RIBOSOME DNA; RNA 16S; TAQ POLYMERASE;

ANALYTIC METHOD; BACTERIAL GENOME; BACTERIUM DETECTION; CHEMISTRY; CONTROLLED STUDY; DAIRY INDUSTRY; DNA DETERMINATION; DOT HYBRIDIZATION; ENZYME ACTIVATION; ENZYME ASSAY; FEASIBILITY STUDY; FECES MICROFLORA; GENE AMPLIFICATION; GENE POOL; GENE TARGETING; GENETIC PROCEDURES; INTERMETHOD COMPARISON; ISOTOPE LABELING; LINEAR SYSTEM; MOLECULAR PROBE; NONHUMAN; OLIGONUCLEOTIDE PROBE; POLYMERASE CHAIN REACTION; POPULATION GENETICS; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REVIEW; SAMPLE SIZE; SENSITIVITY ANALYSIS; SPECIES DIFFERENCE; THERMAL STIMULATION;

BACTERIA; DNA, BACTERIAL; DNA, RIBOSOMAL; FECES; FLUORESCENT DYES; HUMANS; IMMUNOBLOTTING; NUCLEIC ACID HYBRIDIZATION; OLIGONUCLEOTIDE PROBES; ORGANIC CHEMICALS; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; TAQ POLYMERASE;

BACTERIA (MICROORGANISMS);

EID: 0037261792     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/mic.0.25975-0     Document Type: Review

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