Probabilistic methods in directed evolution: Library size, mutation rate, and diversity

Methods in Molecular Biology

Volumn 1179, Issue , 2014, Pages 261-278

  Nov, Yuval ^a  

^a UNIVERSITY OF HAIFA   (Israel)

Author keywords

Computational tools; Directed evolution; DNA shuffling; Error prone PCR; Library generation; Probabilistic modeling; Saturation mutagenesis; Staggered extension process

Indexed keywords

OLIGONUCLEOTIDE; PROTEIN VARIANT;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; CODON USAGE; DIRECTED MOLECULAR EVOLUTION; DNA RECOMBINATION; DNA SEQUENCE; DNA SHUFFLING; DNA TEMPLATE; ERROR PRONE POLYMERASE CHAIN REACTION; GENE LIBRARY; GENETIC CODE; GENETIC VARIABILITY; IN VITRO RECOMBINATION; INDEL MUTATION; MUTAGENESIS; MUTATION RATE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROBABILITY; RANDOMIZATION; REVIEW; SATURATION MUTAGENESIS; STOP CODON; SYSTEM ANALYSIS; PROCEDURES;

DIRECTED MOLECULAR EVOLUTION; GENE LIBRARY; MUTAGENESIS; MUTATION RATE; POLYMERASE CHAIN REACTION;

EID: 84925884241     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-1053-3_18     Document Type: Review

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