Cleavage efficient 2A peptides for high level monoclonal antibody expression in CHO cells

mAbs

Volumn 7, Issue 2, 2015, Pages 403-412

  Chng, Jake ^a   Wang, Tianhua ^a   Nian, Rui ^a   Lau, Ally ^a   Hoi, Kong Meng ^a   Ho, Steven C L ^a   Gagnon, Peter ^a   Bi, Xuezhi ^a   Yang, Yuansheng ^a,b  

^a AGENCY FOR SCIENCE   (Singapore)

^b Nanyang Technological University   (Singapore)

Author keywords

2A peptide; CHO; Cleavage efficiency; Furin; GSG linker; Monoclonal antibody

Indexed keywords

BEVACIZUMAB; BIOSIMILAR AGENT; IMMUNOGLOBULIN G1; MONOCLONAL ANTIBODY; PEPTIDE; TRASTUZUMAB; VIRUS PROTEIN; HYBRID PROTEIN; IMMUNOGLOBULIN G; IMMUNOGLOBULIN HEAVY CHAIN; IMMUNOGLOBULIN LIGHT CHAIN;

ANIMAL CELL; ANTIBODY PRODUCTION; ANTIGEN RECOGNITION; ARTICLE; BACTERIUM CULTURE; CHO CELL LINE; CHROMATIN; CONTROLLED STUDY; EQUINE RHINITIS A VIRUS; FOOT AND MOUTH DISEASE VIRUS; LIQUID CHROMATOGRAPHY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN CLEAVAGE; PROTEIN PURIFICATION; STABLE TRANSFECTION; TANDEM MASS SPECTROMETRY; TESCHOVIRUS; THOSEA ASIGNA VIRUS; TRANSIENT TRANSFECTION; VIRUS STRAIN; ANIMAL; BIOSYNTHESIS; CRICETULUS; GENETICS; HAMSTER; HUMAN; METABOLISM; PROTEIN DEGRADATION;

ANIMALS; ANTIBODIES, MONOCLONAL; CHO CELLS; CRICETINAE; CRICETULUS; HUMANS; IMMUNOGLOBULIN G; IMMUNOGLOBULIN HEAVY CHAINS; IMMUNOGLOBULIN LIGHT CHAINS; PEPTIDES; PROTEOLYSIS; RECOMBINANT FUSION PROTEINS; VIRAL PROTEINS;

EID: 84924585125     PISSN: 19420862     EISSN: 19420870     Source Type: Journal    
DOI: 10.1080/19420862.2015.1008351     Document Type: Article

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