Control of IgG LC: HC ratio in stably transfected CHO cells and study of the impact on expression, aggregation, glycosylation and conformational stability

Journal of Biotechnology

Volumn 165, Issue 3-4, 2013, Pages 157-166

  Ho, Steven C L ^a,b   Koh, Esther Y C ^a   van Beers, Miranda ^a   Mueller, Monika ^a   Wan, Corrine ^a   Teo, Gavin ^a   Song, Zhiwei ^a   Tong, Yen Wah ^b   Bardor, Muriel ^a,c   Yang, Yuansheng ^a,d  

^a AGENCY FOR SCIENCE   (Singapore)

^b NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^c UNIVERSITÉ DE ROUEN   (France)

^d Nanyang Technological University   (Singapore)

Author keywords

Aggregation; Cell line generation; Conformational stability; Glycosylation; Monoclonal antibody

Indexed keywords

CELL LINES; CHINESE HAMSTER OVARY CELLS; CONFORMATIONAL STABILITIES; GENE AMPLIFICATION; IMMUNOGLOBULIN G; INTERNAL RIBOSOME ENTRY SITE; MONOCLONAL ANTIBODIES (MABS); PRODUCT STABILITY;

AGGLOMERATION; CELL CULTURE; COVALENT BONDS; ESTERIFICATION; GENE EXPRESSION; GLYCOSYLATION; LAKES; MONOCLONAL ANTIBODIES; MONOMERS; POLYSACCHARIDES;

CONFORMATIONS;

GLYCAN; IMMUNOGLOBULIN G; IMMUNOGLOBULIN G HEAVY CHAIN; IMMUNOGLOBULIN G LIGHT CHAIN; MONOMER; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ANIMAL CELL; ARTICLE; CHO CELL; DIFFERENTIAL SCANNING CALORIMETRY; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; GENE AMPLIFICATION; GLYCOSYLATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN CONFORMATION; PROTEIN EXPRESSION; PROTEIN STABILITY; WESTERN BLOTTING;

ANIMALS; ANTIBODIES, MONOCLONAL; CHO CELLS; CRICETINAE; CRICETULUS; GENETIC VECTORS; GLYCOSYLATION; HUMANS; IMMUNOGLOBULIN G; IMMUNOGLOBULIN HEAVY CHAINS; IMMUNOGLOBULIN LIGHT CHAINS; INTRACELLULAR SPACE; PROTEIN CONFORMATION; PROTEIN STABILITY; RECEPTOR, ERBB-2; TEMPERATURE;

EID: 84877354063     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2013.03.019     Document Type: Article

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