Composition and arrangement of genes define the strength of IRES-driven translation in bicistronic mRNAs

Nucleic Acids Research

Volumn 29, Issue 16, 2001, Pages 3327-3334

  Hennecke, Meike ^a   Kwissa, Marcin ^b   Metzger, Karin ^b   Oumard, André ^a   Kröger, Andrea ^a   Schirmbeck, Reinhold ^b   Reimann, Jörg ^b   Hauser, Hansjörg ^a  

^a HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

^b UNIVERSITY OF ULM   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOKINE; HEPATITIS B CORE ANTIGEN; LUCIFERASE; MESSENGER RNA;

ANIMAL CELL; ARTICLE; CISTRON; CONTROLLED STUDY; ENCEPHALOMYOCARDITIS VIRUS; ENZYME ASSAY; EUKARYOTE; EXPRESSION VECTOR; GENE EXPRESSION; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; POLIOMYELITIS VIRUS; PRIORITY JOURNAL; RIBOSOME; RNA TRANSLATION; START CODON; TRANSLATION INITIATION;

EID: 0035880772     PISSN: 03051048     EISSN: None     Source Type: Journal    
DOI: 10.1093/nar/29.16.3327     Document Type: Article

References (52)

1. Starting at the beginning, middle and end: Translation initiation in eukaryotes
2. Internal translation initiation in the design of improved expression vectors rfsti Curr. Opin. Biotechnol.
3. Bicistronic transcription units for gene expression in mammalian cells
4. Internal ribosome entry sites and dicistronic RNAs in mammalian transgenesis
5. Internal ribosome entry site biology and its use in expression vectors
6. Equimolar expression of two protein chains in mammalian cells
7. Picornavirus internal ribosome entry segments: Comparison of translation efficiency and the requirements for optimal internal initiation of translation in vitro
8. Identification of mammalian cell clones exhibiting highly regulated expression from inducible promoters
9. Polyvalent vaccination against hepatitis B surface and core antigen using a dicistronic expression plasmid
10. Genetic optimization of recombinant protein production by mammalian cells
11. Internal initiation of translation in retroviral vectors carrying picarnovirus 5′ nontranslated regions
12. Retroviral vectors containing putative internal ribosome entry sites: Development of a polycistronic gene transfer system and applications to human gene therapy
13. High-titer bicistronic retroviral vectors employing foot-and-mouth disease virus internal ribosome entry site
14. Conversion to expression stability by Flp-mediated cassette replacement
15. Internal initiation of translation of eukaryotic mRNA directed by a sequence derived from poliovirus RNA
16. Internal initiation of translation directed by the 5′-untranslated region of the mRNA for eIF4G, a factor involved in the picornavirus-induced switch from cap-dependent to internal initiation
17. Alternative translation of human fibroblast growth factor 2 mRNA occurs by internal entry of ribosomes
18. Unique features of internal initiation of translation of hepatitis C virus translation
19. PDGF2/c-sis mRNA leader contains a differentiation linked internal ribosomal entry site (D-IRES)
20. Ultrabithorax and Antennapedia 5′ untranslated regions promote developmentally regulated internal translation initiation
21. Regulation of vascular endothelial growth factor (VEGF) expression is mediated by internal initiation of translation and alternative initiation of transcription
22. A secreted DNA-binding protein that is translated through an internal ribosome entry site (IRES) and distributed in a discrete pattern in the central nervous system
23. Translation initiation of cardiac voltage-gated potassium channel by internal ribosome entry
24. Translation of vascular endothelial growth factor mRNA by internal ribosome entry: Implications for translation under hypoxia
25. A 9-nt segment of a cellular mRNA can function as an internal ribosome entry site (IRES) and when present in linked multiple copies greatly enhances IRES activity
26. A novel form of DAP5 protein accumulates in apoptotic cells as a result of caspase cleavage and internal ribosome entry site-mediated translation
27. Internal initiation of translation in eukaryotes: The picornavirus paradigm and beyond
28. A common RNA structural motif involved in the internal initiation of translation of cellular mRNAs
29. RNA-protein interactions in regulation of picarnovirus RNA translation
30. Two independent internal ribosome entry sites are involved in translation initiation of vascular endothelial growth factor mRNA
31. Canonical eukaryotic initiation factors determine initiation of translation by internal ribosome entry
32. Recognition of picornavirus internal ribosome entry sites within cells; influence of cellular and viral proteins
33. Comparison of picornaviral-IRES driven internal initiation of translation in cultured cells of different origins
34. Translation of NRF mRNA is mediated by highly efficient internal ribosome entry
35. Polyvalent vectors for coexpression of multiple genes
36. Construction and characterization of retroviral vectors expressing biologically active human interleukin-12
37. pTRIDENT, a novel vector family for tricistronic gene expression in mammalian cells
38. Stabilized, long-term expression of heteromeric proteins from tricistronic mRNA
39. DNA-based vaccination with polycistronic expression plasmids
40. Similar as well as distinct MHC class I-binding peptides are generated by exogenous and endogenous processing of hepatitis B virus surface antigen
41. Studies with MHC-deficient knock-out mice reveal impact of both MHC I and MHC II-dependent T cell responses in Listeria monocytogenes infection
42. Expression of selectin-binding epitopes and cytokines by CD4+ T cells repopulating scid mice with colitis
43. Firefly luciferase gene: Structure and expression in mammalian cells
44. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells
45. High interferon producing line of transformed murine cells
46. Applications of expression vectors containing bicistronic transcription units in mammalian cells
47. mRNA stability in mammalian cells
48. Post-transcriptional gene regulatory mechanisms in eukaryotes: An overview
49. The optimal use of IRES (internal ribosome entry site) in expression vectors
50. Bicistronic vector for the creation of stable mammalian cell lines that predisposes all antibiotics-resistant cells to express recombinant protein
51. A segment of the 5′ nontranslated region of encephalomyocarditis virus RNA directs internal entry of ribosomes during in vitro translation