Mutated polyadenylation signals for controlling expression levels of multiple genes in mammalian cells

Biotechnology and Bioengineering

Volumn 102, Issue 4, 2009, Pages 1152-1160

  Yang, Yuansheng ^a   Mariati, ^a   Ho, Steven C L ^a   Yap, Miranda G S ^a,b  

^a AGENCY FOR SCIENCE   (Singapore)

^b NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

Control of gene expression; Mammalian cells; Monoclonal antibody; RNA processing; SV40 early polyadenylation signal

Indexed keywords

EXPRESSION LEVELS; HEAVY CHAINS; IN FIELDS; LIGHT CHAINS; MAMMALIAN CELL LINES; MAMMALIAN CELLS; METABOLIC ENGINEERINGS; MONOCLONAL ANTIBODIES (MAB); MRNA LEVELS; MULTIPLE GENES; NEW TOOLS; PROTEIN INTERACTIONS; RELATIVE STRENGTHS; REPORTER GENES; RNA PROCESSING; SINGLE VECTORS; SV40 EARLY POLYADENYLATION SIGNAL; TRACE AMOUNTS; TRANSIENT TRANSFECTIONS; WIDE-RANGING APPLICATIONS; WILD TYPES;

BIOACTIVITY; BIOCHEMICAL ENGINEERING; CELL CULTURE; ENGINEERING RESEARCH; ENZYME ACTIVITY; GENE EXPRESSION; MONOCLONAL ANTIBODIES; NUCLEIC ACIDS; PLANTS (BOTANY); RNA; SURFACE PLASMON RESONANCE;

MAMMALS;

LUCIFERASE; MESSENGER RNA; MONOCLONAL ANTIBODY; POLYPEPTIDE;

ACCURACY; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; GENE EXPRESSION REGULATION; GENE MUTATION; GENE SEQUENCE; GENETIC TRANSFECTION; GENETIC VARIABILITY; HUMAN; HUMAN CELL; MAMMAL CELL; METABOLIC ENGINEERING; MOUSE; NONHUMAN; POLYADENYLATION; PROMOTER REGION; PROTEIN INTERACTION; PROTEIN SYNTHESIS; PROTEOMICS; REPORTER GENE; SIGNAL TRANSDUCTION; WILD TYPE; BIOSYNTHESIS; BIOTECHNOLOGY; GENETICS; METHODOLOGY; MUTATION; POLYOMA VIRUS; REGULATORY RNA SEQUENCE; VIRUS GENE;

MAMMALIA;

ANTIBODIES, MONOCLONAL; BIOTECHNOLOGY; GENE EXPRESSION REGULATION; GENES, VIRAL; MUTATION; POLYOMAVIRUS; PROTEIN BIOSYNTHESIS; RNA 3' POLYADENYLATION SIGNALS;

EID: 60549116306     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22152     Document Type: Article

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