Improvement of L-phenylalanine production from glycerol by recombinant Escherichia coli strains: The role of extra copies of glpK, glpX, and tktA genes

Microbial Cell Factories

Volumn 13, Issue 1, 2014, Pages

  Gottlieb, Katrin ^a   Albermann, Christoph ^a   Sprenger, Georg A ^a  

^a UNIVERSITY OF STUTTGART   (Germany)

Author keywords

Crude glycerol; Escherichia coli; Fructose 1,6 bisphosphatase; Glycerol; Glycerol kinase; L phenylalanine; Metabolic engineering; Transketolase

Indexed keywords

CARBON; FRUCTOSE BISPHOSPHATASE; FRUCTOSE BISPHOSPHATE ALDOLASE; GLUCOSE; GLUCOSE 6 PHOSPHATE; GLYCEROL; GLYCEROL KINASE; PHENYLALANINE; PHOSPHOENOLPYRUVATE; PHOSPHOTRANSFERASE; TRANSKETOLASE; ESCHERICHIA COLI PROTEIN;

AROB GENE; AROF GENE; AROL GENE; ARTICLE; BACTERIAL STRAIN; CARBON SOURCE; CONTROLLED STUDY; ESCHERICHIA COLI; GENE; GENE DOSAGE; GENETIC CODE; GLPK GENE; GLPX GENE; GLUCONEOGENESIS; GLYCOLYSIS; GROWTH RATE; NONHUMAN; PHEA GENE; PROTEIN PHOSPHORYLATION; RECOMBINANT GENE; TKTA GENE; BIOSYNTHESIS; ENZYMOLOGY; GENETICS; METABOLIC ENGINEERING; METABOLISM; PENTOSE PHOSPHATE CYCLE;

ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FRUCTOSE-BISPHOSPHATASE; GENE DOSAGE; GLYCEROL; GLYCEROL KINASE; METABOLIC ENGINEERING; PENTOSE PHOSPHATE PATHWAY; PHENYLALANINE; TRANSKETOLASE;

EID: 84923874240     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/s12934-014-0096-1     Document Type: Article

References (74)

1. Almeida JRM, Fávaro LCL, Quirino BF: Biodiesel biorefinery: opportunities and challenges for microbial production of fuels and chemicals from glycerol waste. Biotechnol Biofuels 2012, 5:48.
2. Wendisch VF, Meiswinkel T, Lindner S: Use of glycerol in biotechnological applications. In Biodiesel - Quality, Emissions and By-Products. Edited by Gisela M, Margarita S. Rijeka, Croatia: In Tech - Open Access Publisher; 2011:305-340.
3. Heller KB, Lin ECC, Wilson TH: Substrate specificity and transport properties of the glycerol facilitator of Escherichia coli. J Bacteriol 1980, 144:274-278.
4. Zwaig N, Kistler WS, Lin ECC: Glycerol kinase, the pacemaker for the dissimilation of glycerol in Escherichia coli. J Bacteriol 1970, 102:753-759.
5. Weiner JH, Heppel LA: Purification of the membrane-bound and pyridine nucleotide-independent L-glycerol 3-phosphate dehydrogenase from Escherichia coli. Biochem Biophys Res Commun 1972, 47:1360-1365.
6. Yeh JI, Chinte U, Du S: Structure of glycerol-3-phosphate dehydrogenase, an essential monotopic membrane enzyme involved in respiration and metabolism. Proc Natl Acad Sci U S A 2008, 105:3280-3285.
7. Andersen KB, von Meyenburg K: Are growth rates of Escherichia coli in batch cultures limited by respiration? J Bacteriol 1980, 144:114-123.
8. Soetaert W, Vandamme E: The impact of industrial biotechnology. Biotechnol J 2006, 1:756-769.
9. Postma PW, Lengeler JW, Jacobson GR: Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria. Microbiol Rev 1993, 57:543-594.
10. Gosset G: Improvement of Escherichia coli production strains by modification of the phosphoenolpyruvate:sugar phosphotransferase system. Microb Cell Fact 2005, 4:14.
11. Martínez-Gómez K, Flores N, Castañeda HM, Martínez-Batallar G, Hernández-Chávez G, Ramírez OT, Gosset G, Encarnación S, Bolivar F: New insights into Escherichia coli metabolism: carbon scavenging, acetate metabolism and carbon recycling responses during growth on glycerol. Microb Cell Fact 2012, 11:46.
12. Neidhardt FC, Ingraham JL, Schaechter M: Physiology of the Bacterial Cell: A Molecular Approach. Sunderland, Mass: Sinauer Associates; 1990.
13. Ibarra RU, Edwards JS, Palsson BO: Escherichia coli K-12 undergoes adaptive evolution to achieve in silico predicted optimal growth. Nature 2002, 420:186-189.
14. Lin ECC: Glycerol dissimilation and its regulation in bacteria. Annu Rev Microbiol 1976, 30:535-578.
15. Berman M, Lin ECC: Glycerol-specific revertants of a phosphoenolpyruvate phosphotransferase mutant: suppression by the desensitization of glycerol kinase to feedback inhibition. J Bacteriol 1971, 105:113-120.
16. Holtman CK, Pawlyk AC, Meadow ND, Pettigrew DW: Reverse genetics of Escherichia coli glycerol kinase allosteric regulation and glucose control of glycerol utilization in vivo. J Bacteriol 2001, 183:3336-3344.
17. Lin ECC: Dissimilatory pathways for sugars, polyols, and carboxylates. In Escherichia coli and Salmonella. 2nd edition. Edited by Neidhardt FC. Washington, D.C: ASM Press; 1996:307-342.
18. Marr AG: Growth rate of Escherichia coli. Microbiol Rev 1991, 55:316-333.
19. Fraenkel DG, Horecker BL: Fructose-1, 6-diphosphatase and acid hexose phosphatase of Escherichia coli. J Bacteriol 1965, 90:837-842.
20. Sedivy JM, Daldal F, Fraenkel DG: Fructose bisphosphatase of Escherichia coli: cloning of the structural gene ( fbp ) and preparation of a chromosomal deletion. J Bacteriol 1984, 158:1048-1053.
21. Donahue JL, Bownas JL, Niehaus WG, Larson TJ: Purification and characterization of glpX -encoded fructose 1, 6-bisphosphatase, a new enzyme of the glycerol 3-phosphate regulon of Escherichia coli. J Bacteriol 2000, 182:5624-5627.
22. Taymaz-Nikerel H, Borujeni AE, Verheijen PJT, Heijnen JJ, van Gulik WM: Genome-derived minimal metabolic models for Escherichia coli MG1655 with estimated in vivo respiratory ATP stoichiometry. Biotechnol Bioeng 2010, 107:369-381.
23. Pittard JA: Biosynthesis of the aromatic amino acids. I. In Escherichia coli and Salmonella. 2nd edition. Edited by Neidhardt FC. Washington, D.C: ASM Press; 1996:458-484.
24. Sprenger GA: Aromatic Amino Acids. In Amino Acid Biosynthesis ~ Pathways, Regulation and Metabolic Engineering. Edited by Wendisch VF. Berlin, Heidelberg: Springer Berlin Heidelberg; 2007:93-127 [Microbiology Monographs].
25. Sprenger GA: From scratch to value: engineering Escherichia coli wild type cells to the production of L-phenylalanine and other fine chemicals derived from chorismate. Appl Microbiol Biotechnol 2007, 75:739-749.
26. Draths KM, Frost JW: Synthesis using plasmid-based biocatalysis: plasmid assembly and 3-deoxy-D-arabino-heptulosonate production. J Am Chem Soc 1990, 112:1657-1659.
27. Sprenger G, Siewe R, Sahm H, Karutz M, Sonke T: Microbial Preparation of Substances from Aromatic Metabolism/I US Patent 09/298,843. ; 1998.
28. Sprenger GA: Genetics of pentose-phosphate pathway enzymes of Escherichia coli K-12. Arch Microbiol 1995, 164:324-330.
29. Sprenger GA, Schörken U, Sprenger G, Sahm H: Transketolase A of Escherichia coli K12. Purification and properties of the enzyme from recombinant strains. Eur J Biochem 1995, 230:525-532.
30. Rui D, Yonghua A: Development of L-phenylalanine in China. China Chem Rep 2011, 22:19-20.
31. Weiner M, Albermann C, Gottlieb K, Sprenger GA, Weuster-Botz D: Fed-batch production of l-phenylalanine from glycerol and ammonia with recombinant Escherichia coli. Biochem Eng J 2014, 83:62-69.
32. Varma A, Boesch BW, Palsson BO: Biochemical production capabilities of Escherichia coli. Biotechnol Bioeng 1993, 42:59-73.
33. Khamduang M, Packdibamrung K, Chutmanop J, Chisti Y, Srinophakun P: Production of L-phenylalanine from glycerol by a recombinant Escherichia coli. J Ind Microbiol Biotechnol 2009, 36:1267-1274.
34. Thongchuang M, Pongsawasdi P, Chisti Y, Packdibamrung K: Design of a recombinant Escherichia coli for producing L-phenylalanine from glycerol. World J Microbiol Biotechnol 2012, 28:2937-2943.
35. Srinophakun P, Reakasame S, Khamduang M, Packdibamrung K, Thanapi A: Potential of L-phenylalanine production from raw glycerol of palm biodiesel process by a recombinant Escherichia coli. Chiang Mai J Science 2012, 39:59-68.
36. Gosset G: Production of aromatic compounds in bacteria. Curr Opin Biotechnol 2009, 20:651-658.
37. Zeppenfeld T, Larisch C, Lengeler JW, Jahreis K: Glucose transporter mutants of Escherichia coli K-12 with changes in substrate recognition of IICB(Glc) and induction behavior of the ptsG gene. J Bacteriol 2000, 182:4443-4452.
38. Rüffer N, Heidersdorf U, Kretzers I, Sprenger GA, Raeven L, Takors R: Fully integrated L-phenylalanine separation and concentration using reactive-extraction with liquid-liquid centrifuges in a fed-batch process with E. coli. Bioprocess Biosyst Eng 2004, 26:239-248.
39. Gerigk M, Bujnicki R, Ganpo-Nkwenkwa E, Bongaerts J, Sprenger G, Takors R: Process control for enhanced L-phenylalanine production using different recombinant Escherichia coli strains. Biotechnol Bioeng 2002, 80:746-754.
40. Gerigk MR, Maass D, Kreutzer A, Sprenger G, Bongaerts J, Wubbolts M, Takors R: Enhanced pilot-scale fed-batch L-phenylalanine production with recombinant Escherichia coli by fully integrated reactive extraction. Bioprocess Biosyst Eng 2002, 25:43-52.
41. Gottlieb K: Nutzung von Glycerin und Rohglycerin als C-Quelle für die Produktion von L-Phenylalanin mit rekombinanten Escherichia coli -Stämmen. In PhD Thesis. Stuttgart, Germany: Universität Stuttgart, Institut für Mikrobiologie; 2011.
42. Rodrigues AL, Trachtmann N, Becker J, Lohanatha AF, Blotenberg J, Bolten CJ, Korneli C, de Souza L, André O, Porto LM, Sprenger GA, Wittmann C: Systems metabolic engineering of Escherichia coli for production of the antitumor drugs violacein and deoxyviolacein. Metab Eng 2013, 20:29-41.
43. Weiner M, Tröndle J, Albermann C, Sprenger GA, Weuster-Botz D: Improvement of constraint-based flux estimation during L-phenylalanine production with Escherichia coli using targeted knock-out mutants. Biotechnol Bioeng 2014, 111:1406-1416.
44. Bachmann BJ: Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol Rev 1972, 36:525-557.
45. Datsenko KA, Wanner BL: One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci U S A 2000, 97:6640-6645.
46. Cherepanov PP, Wackernagel W: Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant. Gene 1995, 158:9-14.
47. Furste JP, Pansegrau W, Frank R, Blocker H, Scholz P, Bagdasarian M, Lanka E: Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vector. Gene 1986, 48:119-131.
48. Honisch C: High-throughput mutation detection underlying adaptive evolution of Escherichia coli -K12. Genome Res 2004, 14:2495-2502.
49. Anderson MJ, DeLaBarre B, Raghunathan A, Palsson BO, Brunger AT, Quake SR: Crystal structure of a hyperactive Escherichia coli glycerol kinase mutant Gly230 →Asp obtained using microfluidic crystallization devices. Biochemistry 2007, 46:5722-5731.
50. El-Mansi M: Flux to acetate and lactate excretions in industrial fermentations: physiological and biochemical implications. J Ind Microbiol Biotechnol 2004, 31:295-300.
51. Albermann C, Trachtmann N, Sprenger GA: A simple and reliable method to conduct and monitor expression cassette integration into the Escherichia coli chromosome. Biotechnol J 2010, 5:32-38.
52. Lennox ES: Transduction of linked genetic characters of the host by bacteriophage P1. Virology 1955, 1:190-206.
53. Thierauf A, Perez G, Maloy AS: Generalized transduction. Methods Mol Biol 2009, 501:267-286.
54. Truniger V, Boos W, Sweet G: Molecular analysis of the glpFKX regions of Escherichia coli and Shigella flexneri. J Bacteriol 1992, 174:6981-6991.
55. Col B: Regulation of Fructose 1,6-bisphosphatase II (GlpX) Gene Expression in Escherichia coli. In PhD Thesis. Blacksburg, VA, USA: Virginia Polytechnic Institute State University; 2004.
56. Iida A, Teshiba S, Mizobuchi K: Identification and characterization of the tktB gene encoding a second transketolase in Escherichia coli K-12. J Bacteriol 1993, 175:5375-5383.
57. Eidels L, Osborn MJ: Lipopolysaccharide and aldoheptose biosynthesis in transketolase mutants of Salmonella typhimurium. Proc Natl Acad Sci U S A 1971, 68:1673-1677.
58. Martínez K, de Anda R, Hernández G, Escalante A, Gosset G, Ramírez OT, Bolívar FG: Coutilization of glucose and glycerol enhances the production of aromatic compounds in an Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system. Microb Cell Fact 2008, 7:1.
59. Yazdani SS, Gonzalez R: Anaerobic fermentation of glycerol: a path to economic viability for the biofuels industry. Curr Opin Biotechnol 2007, 18:213-219.
60. Sakamoto N, Kotre AM, Savageau MA: Glutamate dehydrogenase from Escherichia coli: purification and properties. J Bacteriol 1975, 124:775-783.
61. Khanna S, Goyal A, Moholkar VS: Microbial conversion of glycerol: present status and future prospects. Crit Rev Biotechnol 2012, 32:235-262.
62. Ahn JO, Lee HW, Saha R, Park MS, Jung J, Lee D: Exploring the effects of carbon sources on the metabolic capacity for shikimic acid production in Escherichia coli using in silico metabolic predictions. J Microbiol Biotechnol 2008, 18:1773-1784.
63. Chen K, Dou J, Tang S, Yang Y, Wang H, Fang H, Zhou C: Deletion of the aroK gene is essential for high shikimic acid accumulation through the shikimate pathway in E. coli. Bioresour Technol 2012, 119:141-147.
64. Ikeda M: Towards bacterial strains overproducing L-tryptophan and other aromatics by metabolic engineering. Appl Microbiol Biotechnol 2006, 69:615-626.
65. Iuchi S, Lin ECC: arcA ( dye ), a global regulatory gene in Escherichia coli mediating repression of enzymes in aerobic pathways. Proc Natl Acad Sci U S A 1988, 85:1888-1892.
66. Iuchi S, Weiner L: Cellular and molecular physiology of Escherichia coli in the adaptation to aerobic environments. J Biochem 1996, 120:1055-1063.
67. Berríos-Rivera SJ, Sánchez AM, Bennett GN, San K: Effect of different levels of NADH availability on metabolite distribution in Escherichia coli fermentation in minimal and complex media. Appl Microbiol Biotechnol 2004, 65:426-432.
68. Hines JK, Fromm HJ, Honzatko RB: Novel allosteric activation site in Escherichia coli fructose-1,6-bisphosphatase. J Biol Chem 2006, 281:18386-18393.
69. Hines JK, Kruesel CE, Fromm HJ, Honzatko RB: Structure of inhibited fructose-1,6-bisphosphatase from Escherichia coli: distinct allosteric inhibition sites for AMP and glucose 6-phosphate and the characterization of a gluconeogenic switch. J Biol Chem 2007, 282:24697-24706.
70. Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual. 2nd edition. Cold Spring Harbor, N.Y: Cold Spring Harbor Laboratory; 1989.
71. Gerhardt P: Methods for General and Molecular Bacteriology. Washington, D.C: American Society for Microbiology; 1994.
72. Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976, 72:248-254.
73. Altmann HJ, Fürstenau E, Gielewski A, Scholz L: Photometrische Bestimmung kleiner Phosphatmengen mit Malachitgrün. Z Anal Chem 1971, 256:274-276.
74. Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227:680-685.