Crystal structure of a hyperactive Escherichia coli glycerol kinase mutant Gly230 → Asp obtained using microfluidic crystallization devices

Biochemistry

Volumn 46, Issue 19, 2007, Pages 5722-5731

  Anderson, Megan J ^a,b   DeLaBarre, Byron ^c   Raghunathan, Anu ^d   Palsson, Bernhard O ^d   Brunger, Axel T ^c   Quake, Stephen R ^b  

^a CALIFORNIA INSTITUTE OF TECHNOLOGY   (United States)

^b Stanford University   (United States)

^c STANFORD UNIVERSITY   (United States)

^d UNIVERSITY OF CALIFORNIA SAN DIEGO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CRYSTAL STRUCTURE; CRYSTALLIZATION; ESCHERICHIA COLI; GLYCEROL; MICROFLUIDICS; MUTAGENS;

ALLOSTERIC REGULATION; DIFFRACTION-QUALITY CRYSTALS; MICROFLUIDIC DEVICES;

ENZYME ACTIVITY;

ASPARTIC ACID; FRUCTOSE 1,6 BISPHOSPHATE; GLYCEROL; GLYCEROL KINASE; GLYCINE; NUCLEOTIDE; TETRAMER;

ARTICLE; BINDING SITE; CONTROLLED STUDY; CRYSTAL STRUCTURE; CRYSTALLIZATION; DATA COLLECTION METHOD; DEVICE; DIFFRACTION; DIFFUSION; ENZYME ACTIVITY; ENZYME BINDING; ENZYME CONFORMATION; ENZYME SUBSTRATE; ESCHERICHIA COLI; LIGHT SCATTERING; MICROFLUIDIC ANALYSIS; MICROFLUIDICS; MOLECULAR INTERACTION; MUTANT; NONHUMAN; PRIORITY JOURNAL; SOLUBILITY; STRUCTURE ANALYSIS; WILD TYPE;

AMINO ACID SUBSTITUTION; BINDING SITES; CHROMATOGRAPHY, GEL; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; ESCHERICHIA COLI; FRUCTOSEDIPHOSPHATES; GLYCEROL KINASE; LASERS; MICROFLUIDIC ANALYTICAL TECHNIQUES; MODELS, MOLECULAR; PROTEIN CONFORMATION; PROTEIN STRUCTURE, TERTIARY; SCATTERING, RADIATION; SOLUBILITY;

ESCHERICHIA COLI;

EID: 34248585864     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi700096p     Document Type: Article

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