Fed-batch production of l-phenylalanine from glycerol and ammonia with recombinant Escherichia coli

Biochemical Engineering Journal

Volumn 83, Issue , 2014, Pages 62-69

  Weiner, Michael ^a   Albermann, Christoph ^b   Gottlieb, Katrin ^b   Sprenger, Georg A ^b   Weuster Botz, Dirk ^a  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^b UNIVERSITY OF STUTTGART   (Germany)

Author keywords

Escherichia coli; Fed batch; Glycerol; L Phenylalanine; Plasmid stability

Indexed keywords

FED BATCHES; FED-BATCH PRODUCTION; L-PHENYLALANINE; PHOSPHOENOLPYRUVATES; PLASMID INSTABILITY; PLASMID STABILITY; RECOMBINANT ESCHERICHIA COLI; SPECIFIC PRODUCTIVITY;

AMMONIA; BATCH DATA PROCESSING; DNA; ENZYMES; ESCHERICHIA COLI; FEEDING; GENES; GLYCEROL; LACTIC ACID;

AMINO ACIDS;

ACETIC ACID; AMMONIA; AMPICILLIN; GLYCEROL; KANAMYCIN; LACTIC ACID; PHENYLALANINE; PHOSPHOENOLPYRUVATE; PYRUVATE KINASE; SHIKIMIC ACID; TYROSINE;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BIOMASS PRODUCTION; CARBON SOURCE; CONCENTRATION (PARAMETERS); ESCHERICHIA COLI; FEEDING; GENE CASSETTE; GLYCOLYSIS; NONHUMAN; PENICILLIN RESISTANCE; PLASMID; PRIORITY JOURNAL;

EID: 84891475027     PISSN: 1369703X     EISSN: 1873295X     Source Type: Journal    
DOI: 10.1016/j.bej.2013.12.001     Document Type: Article

References (35)

1. Martínez-Gómez K., Flores N., Castañeda H.M., Martínez-Batallar G., Ramírez O.T., Gosset G., Encarnación S., Bolivar F. New insights into Escherichia coli metabolism: carbon scavenging, acetate metabolism and carbon recycling responses during growth on glycerol. Microb. Cell Fact. 2012, 11:46.
2. Sprenger G.A. From scratch to value: engineering Escherichia coli wild type cells to the production of l-phenylalanine and other fine chemicals derived from chorismate. Appl. Microbiol. Biotechnol. 2007, 75:739-749.
3. Ikeda M. Towards bacterial strains overproducing l-tryptophan and other aromatics by metabolic engineering. Appl. Microbiol. Biotechnol. 2006, 69:615-626.
4. Bongaerts J., Krämer M., Müller U., Raeven L., Wubbolts M. Metabolic engineering for microbial production of aromatic amino acids and derived compounds. Metab. Eng. 2001, 3(4):289-300.
5. Khamduang M., Packdibamrung K., Chutmanop J., Chisti Y., Srinophakun P. Production of l-phenylalanine from glycerol by a recombinant Escherichia coli. J. Ind. Microbiol. Biotechnol. 2009, 36(10):1267-1274.
6. Rüffer N., Heidersdorf U., Kretzers I., Sprenger G.A., Raeven L., Takors R. Fully integrated l-phenylalanine separation and concentration using reactive-extraction with liquid-liquid centrifuges in a fed-batch process with E. coli. Bioprocess Biosyst. Eng. 2004, 26:239-248.
7. Konstantinov K.B., Nishio N., Seki T., Yoshida T. Physiologically motivated strategies for control of the fed-batch cultivation of recombinant Escherichia coli for phenylalanine production. J. Ferment. Bioeng. 1991, 71:350-355.
8. Backman K., O'Connor M.J., Maruya A., Rudd E., McKay D., Balakrishnan R., Radjai M., DiPasquantonio V., Shoda D., Hatch R., Venkatasubramanian K. Genetic engineering of metabolic pathways applied to the production of phenylalanine. Ann. N. Y. Acad. Sci. 1990, 589:16-24.
9. Martínez K., de Anda R., Hernández G., Escalante A., Gosset G., Ramírez O.T., Bolívar F.G. Coutilization of glucose and glycerol enhances the production of aromatic compounds in an Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system. Microb. Cell Fact. 2008, 7:1.
10. Thongchuang M., Chisti Y., Pongsawasdi P., Packdibamrung K. Design of a recombinant Escherichia coli for producing l-phenylalanine from glycerol. World J. Microbiol. Biotechnol. 2012, 28:2937-2943.
11. Gottlieb K. Nutzung von Glycerin und Rohglycerin als C-Quelle fürdie Produktion von L-Phenylalanin mit rekombinanten Escherichia coli-Stämmen 2011, (Ph.D. dissertation), University of Stuttgart.
12. Yazdani S.S., Gonzalez R. Anaerobic fermentation of glycerol: a path to economic viability for the biofuels industry. Curr. Opin. Biotechnol. 2007, 18:213-219.
13. Thompson J.C., He B.B. Characterization of crude glycerol from biodiesel production from multiple feedstocks. Appl. Eng. Agric. 2006, 22(2):261-265.
14. Nicol R.W., Marchand K., Lubitz W.D. Bioconversion of crude glycerol by fungi. Appl. Microbiol. Biotechnol. 2012, 93:1865-1875.
15. Yazdani S.S., Gonzalez R. Engineering Escherichia coli for the efficient conversion of glycerol to ethanol and co-products. Metab. Eng. 2008, 10(6):340-351.
16. Lu D., Grayson P., Schulten K. Glycerol conductance and physical asymmetry of the Escherichia coli glycerol facilitator GlpF. Biophys. J. 2003, 85(5):2977-2987.
17. Heller K.B., Lin E.C.C., Wilson T.H. Substrate specificity and transport properties of the glycerol facilitator of Escherichia coli. J. Bacteriol. 1980, 144:274-278.
18. Meza E., Becker J., Bolivar F., Gosset G., Wittmann C. Consequences of phosphoenolpyruvate:sugarphosphotranferase system and pyruvate kinase isozymes inactivation in central carbon metabolism flux distribution in Escherichia coli. Microb. Cell Fact. 2012, 11:127.
19. Gerigk M., Bujnicki R., Ganpo-Nkwenkwa E., Bongaerts J., Sprenger G., Takors R. Process control for enhanced l-phenylalanine production using different recombinant Escherichia coli strains. Biotechnol. Bioeng. 2002, 80(7):746-754.
20. Datsenko K.A., Wanner B.L. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U. S. A. 2000, 97:6640-6645.
21. Ponce E., Flores N., Martinez A., Valle F., Bolívar F. Cloning of the two pyruvate kinase isoenzyme structural genes from Escherichia coli: the relative roles of these enzymes in pyruvate biosynthesis. J. Bacteriol. 1995, 177(19):5719-5722.
22. Ponce E., Martinez A., Bolivar F., Valle F. Stimulation of glucose catabolism through the pentose pathway by the absence of the two pyruvate kinase isoenzymes in Escherichia coli. Biotechnol. Bioeng. 1998, 58(2-3):292-295.
23. G. Sprenger, et al., Microbial preparation of substances from aromatic metabolism/I, WO98/18936 (1998).
24. R cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant. Gene 1995, 158:9-14.
25. Sharan S.K., Thomason L.C., Kuznetsov S.G., Court D.L. Recombineering: a homologous recombination-based method of genetic engineering. Nat. Protoc. 2009, 4:206-223.
26. Albermann C., Trachtmann N., Sprenger G.A. A simple and reliable method to conduct and monitor expression cassette integration into the Escherichia coli chromosome. Biotechnol. J. 2010, 5(1):32-38.
27. Albermann C., Ghanegaonkar S., Lemuth K., Vallon T., Reuss M., Armbruster W., Sprenger G.A. Biosynthesis of the vitamin E compound δ-tocotrienol in recombinant Escherichia coli cells. Chembiochem 2008, 9:2524-2533.
28. Pan J.G., Rhee J.S., Lebeault J.M. Physiological constraints in increasing biomass concentration of Escherichia coli B in fed-batch culture. Biotechnol. Lett. 1987, 9(2):89-94.
29. Hiller J., Franco-Lara E., Papaioannou V., Weuster-Botz D. Fast sampling and quenching procedures for microbial metabolic profiling. Biotechnol. Lett. 2007, 29:1161-1167.
30. Jenzsch M., Gnoth S., Beck M., Kleinschmidt M., Simutis R., Lübbert A. Open-loop control of the biomass concentration within the growth phase of recombinant protein production processes. J. Biotechnol. 2006, 127:84-94.
31. Ibrahim S.A., Yang H., Seo C.W. Antimicrobial activity of lactic acid and copper on growth of Salmonella and Escherichia coli O157:H7 in laboratory medium and carrot juice. Feed Chem. 2007, 109:137-143.
32. Jossek R., Bongaerts J., Sprenger G.A. Characterization of a new feedback-resistant 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase of Escherichia coli. FEMS Microbiol. Lett. 2001, 202:145-148.
33. Georgiou G., Shuler M.L., Wilson D.B. Release of periplasmic enzymes and other physiological effects of β-lactamase overproduction in Escherichia coli. Biotechnol. Bioeng. 1988, 32:741-748.
34. Friehs K. Plasmid copy number and plasmid stability. Adv. Biochem. Eng. Biotechnol. 2004, 86:47-82.
35. Link H., Anselment B., Weuster-Botz D. Rapid media transition: an experimental approach for steady state analysis of metabolic pathways. Biotechnol. Prog. 2010, 26(1):1-10.