Design of a recombinant Escherichia coli for producing l-phenylalanine from glycerol

World Journal of Microbiology and Biotechnology

Volumn 28, Issue 10, 2012, Pages 2937-2943

  Thongchuang, Mayura ^a   Pongsawasdi, Piamsook ^a   Chisti, Yusuf ^b   Packdibamrung, Kanoktip ^a  

^a CHULALONGKORN UNIVERSITY   (Thailand)

^b MASSEY UNIVERSITY   (New Zealand)

Author keywords

Aromatic amino acid exporter; Bacillus lentus; Escherichia coli BL21(DE3); Glycerol facilitator; l Phenylalanine; Phenylalanine dehydrogenase

Indexed keywords

AERATION RATE; AGITATION SPEED; AROMATIC AMINO ACID; CARBON SOURCE; COEXPRESSED; E. COLI; L-PHENYLALANINE; MAXIMUM PRODUCTION RATE; MAXIMUM VALUES; PHENYLALANINE DEHYDROGENASE; PRODUCTION RATES; RECOMBINANT ESCHERICHIA COLI; SHAKE FLASKS; THERMOTOLERANT;

AROMATIC COMPOUNDS; BACTERIOLOGY; CARBON; CLONE CELLS; CLONING; ESCHERICHIA COLI; FERMENTERS; GENE ENCODING; GLUCOSE; GLYCEROL; SUGAR (SUCROSE);

AMINO ACIDS;

AMINO ACID TRANSPORTER; AQUAPORIN; ESCHERICHIA COLI PROTEIN; GLPF PROTEIN, E COLI; GLYCEROL; OXIDOREDUCTASE; PHENYLALANINE; PHENYLALANINE OXIDASE; RECOMBINANT PROTEIN; YDDG PROTEIN, E COLI;

ARTICLE; BACILLUS; BIOREACTOR; BIOSYNTHESIS; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; METABOLISM; MOLECULAR CLONING; TRANSGENIC ORGANISM;

AMINO ACID OXIDOREDUCTASES; AMINO ACID TRANSPORT SYSTEMS, NEUTRAL; AQUAPORINS; BACILLUS; BIOREACTORS; CLONING, MOLECULAR; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GLYCEROL; ORGANISMS, GENETICALLY MODIFIED; PHENYLALANINE; RECOMBINANT PROTEINS;

BACILLUS LENTUS; EGGERTHELLA LENTA; ESCHERICHIA COLI;

EID: 84865778481     PISSN: 09593993     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11274-012-1104-4     Document Type: Article

References (18)

1. Asano Y, Nakazawa A, Kondo K (1987) Bacillus phenylalanine dehydrogenase produced in E. coli, its purification and application to l-phenylalanine synthesis. Agric Biol Chem 51: 2621-2623.
2. Bollag DM, Rozycki MD, Edelstein SJ (1996) Protein Methods, 2nd edn. Wiley-Liss, New York.
3. Bondioli P, Bella LD (2005) An alternative spectrophotometric method for the determination of free glycerol in biodiesel. Eur J Lipid Sci Technol 107: 153-157. doi: 10. 1002/ejlt. 200401054.
4. Bongaerts J, Krämer M, Müller U, Raeven L, Wubbolts M (2001) Metabolic engineering for microbial production of aromatic amino acids and derived compounds. Metab Eng 3: 289-300. doi: 10. 1006/mben. 2001. 0196.
5. Brunhuber NMW, Blanchard JS (1994) The biochemistry and enzymology of amino acid dehydrogenases. Crit Rev Biochem Mol Biol 29: 415-467. doi: 10. 3109/10409239409083486.
6. da Silva GP, Mack M, Contiero J (2009) Glycerol: a promising and abundant carbon source for industrial microbiology. Biotechnol Adv 27: 30-39. doi: 10. 1016/j. biotechadv. 2008. 07. 006.
7. Doroshenko V, Airich L, Vitushkina M, Kolokolova A, Livshits V, Mashko S (2007) YddG from Escherichiacoli promotes export of aromatic amino acids. FEMS Microbiol Lett 275: 312-318. doi: 10. 1111/j. 1574-6968. 2007. 00894. x.
8. Hori H, Yoneyama H, Tobe R, Ando T, Isogai E, Katsumata R (2011) Inducible l-alanine exporter encoded by the novel gene ygaW (alaE) in Escherichia coli. Appl Environ Microbiol 77: 4027-4034. doi: 10. 1128/AEM. 00003-11.
9. Inkure S (2005) Purification and characterization of phenylalanine dehydrogenase from Bacillus lentus. Dissertation, Chulalongkorn University, Thailand.
10. Khamduang M, Packdibamrung K, Chutmanop J, Chisti Y, Srinophakun P (2009) Production of l-phenylalanine from glycerol by a recombinant Escherichia coli. J Ind Microbiol Biotechnol 36: 1267-1274. doi: 10. 1007/s10295-009-0606-z.
11. Law J, Lee S, Tsen A, Tsul KW, Yu N (2002) The role of glycerol and isopropyl thiogalactoside in Escherichia coli growth and lactose induction of β-galactosidase. J Exp Microbiol Immunol 2: 97-102.
12. Leuchtenberger W, Huthmacher K, Drauz K (2005) Biotechnological production of amino acids and derivatives: current status and prospects. Appl Microbiol Biotechnol 69: 1-8. doi: 10. 1007/s00253-005-0155-y.
13. Lu D, Grayson P, Schulten K (2003) Glycerol conductance and physical asymmetry of the Escherichia coli glycerol facilitator GlpF. Biophys J 85: 2977-2987.
14. Mierendorf R, Yeager K, Novy R (1994) Advance products and protocols for molecular biology research. The pET System: your choice for expression. Innovations newsletter of Novagen, Inc. http://www. biol. sc. edu/~vogt/LAP/pET%20-%20company%20flier%201. pdf. Accessed 15 January 2012.
15. Seol W, Shatkin AJ (1992) Escherichia coli α-ketoglutarate permease is constitutively expressed proton symporter. J Biol Chem 267: 6409-6413.
16. Simmonds S, Dowling MT, Stone D (1954) Metabolism of phenylalanine and tyrosine by Escherichia coli strain K-12. J Biol Chem 208: 701-724.
17. Thongchuang M (2006) Cloning and expression of phenylalanine dehydrogenase from Bacillus lentus. Dissertation, Chulalongkorn University, Thailand.
18. Weckbecker A, Hummel W (2004) Glucose dehydrogenase for the regeneration of NADH and NADPH. In: Barredo JL (ed) Methods in Biotechnology, vol 17: microbial enzymes and biotransformations. Humana Press Inc., Totowa, pp 241-253.