Exploring redundancy in the yeast genome: An improved strategy for use of the cre-loxP system

Gene

Volumn 252, Issue 1-2, 2000, Pages 127-135

  Delneri, Daniela ^a   Tomlin, Gregory C ^a   Wixon, Joanne L ^a   Hutter, Anton ^a   Sefton, Mark ^a   Louis, Edward J ^b   Oliver, Stephen G ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

^b UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

Counter selectable markers; Cycloheximide resistance gene; Gene families; Multiple deletions

Indexed keywords

ARTICLE; EXPRESSION VECTOR; GENE DELETION; GENE DISRUPTION; GENETIC MARKER; MULTIGENE FAMILY; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE;

BLOTTING, SOUTHERN; CHROMOSOMES, FUNGAL; DNA, FUNGAL; DNA, RECOMBINANT; ELECTROPHORESIS, GEL, PULSED-FIELD; GENE DELETION; GENES, FUNGAL; GENETIC MARKERS; GENOME, FUNGAL; INTEGRASES; MULTIGENE FAMILY; PLASMIDS; RECOMBINATION, GENETIC; SACCHAROMYCES CEREVISIAE; VIRAL PROTEINS;

CANDIDA MALTOSA; FUNGI; MYXOGASTRIA; SACCHAROMYCES CEREVISIAE;

EID: 0034636716     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(00)00217-1     Document Type: Article

References (30)

1. Alani E., Cao L., Kleckner N. A method for gene disruption that allows repeated use of URA3 selection in the construction of multiply disrupted yeast strains. Genetics. 116:1987;541-545.
2. André B. An overview of membrane transport proteins in Saccharomyces cerevisiae. Yeast. 11:1995;1575-1611.
3. Baganz F., Hayes A., Marren D., Gardner D.C.J., Oliver S.G. Suitability of replacement markers for functional analysis studies in Saccharomyces cerevisiae. Yeast. 13:1997;1563-1573.
4. Baudin A., Ozier-Kalogeropoulos O., Denouel A., Lacroute F., Cullin C. A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae. Nucleic Acids Res. 21:1993;3329-3330.
5. Chattoo B.B., Sherman F., Azubalis D.A., Fjellstedt T.A., Mehnert D., Ogur M. Selection of lys2 mutants of the yeast Saccharomyces cerevisiae by the utilization of α-aminoadipate. Genetics. 93:1979;51-65.
6. Delneri D., Gardner D.C.J., Bruschi C.V., Oliver S.G. Disruption of seven hypothetical aryl-alcohol dehydrogenase genes from Saccharomyces cerevisiae and construction of a multiple knock-out strain. Yeast. 15:1999;1681-1689.
7. Delneri D., Gardner D.C.J., Oliver S.G. Analysis of the seven-member AAD gene set demonstrates that genetic redundancy in yeast may be more apparent than real. Genetics. 153:1999;1591-1600.
8. Dujon B. The yeast genome project: what did we learn? Trends Genet. 12:1996;263-270.
9. Fairhead C., Llorente B., Denis F., Soler M., Dujon B. New vectors for combinatorial deletions in yeast chromosomes and for gap-repair cloning using 'split-marker' recombination. Yeast. 12:1996;1439-1458.
10. Fleig U.N., Pridmore R.D., Philippsen P. Construction of LYS2 cartridges for use in genetic manipulations of Saccharomyces cerevisiae. Gene. 46:1986;237-245.
11. Gardner D.C.J., Heale S.M., Stateva L.I., Oliver S.G. Treatment of yeast cells with wall lytic enzymes is not required to prepare chromosomes for pulsed-field gel analysis. Yeast. 9:1993;1053-1055.
12. Goffeau A., Barrell B.G., Bussey H., Davis R.W., Dujon B., Feldmann H., Galibert F., Hoheisel J.D., Jacq C., Johnston M., Louis E.J., Mewes H.W., Murakami Y., Philippsen P., Tettelin H., Oliver S.G. Life with 6000 genes. Science. 274:1996;546-567.
13. Goldstein A.L., McCusker J.H. Three new dominant drug resistance cassettes for gene disruption in Saccharomyces cerevisiae. Yeast. 15:1999;1541-1553.
14. Güldener U., Heck S., Fiedler T., Beinhauer J., Hegemann J.H. A new efficient gene disruption cassette for repeated use in budding yeast. Nucleic Acids Res. 24:1996;2519-2524.
15. Hashida-Okado T., Ogawa A., Endo M., Yasumoto R., Takesako K., Kato I. AUR1, a novel gene conferring aureobasidin resistance on Saccharomyces cerevisiae: a study of defective morphologies in Aur1p-depleted cells. Mol. Gen. Genet. 251:1996;236-244.
16. Hutter A., Oliver S.G. Ethanol production using nuclear petite yeast mutants. Appl. Microbiol. Biotechnol. 49:1998;511-516.
17. Kaiser C., Michaelis G., Mitchell A. Methods in Yeast Genetics. 1994;Cold Spring Harbor Press, Cold Spring Harbor, NY.
18. Längle-Rouault F., Jacobs E. A method for performing precise alterations in the yeast genome using a recyclable selectable marker. Nucleic Acids Res. 23:1995;3079-3081.
19. McCusker J.H., Davis R.W. The use of proline as a nitrogen source causes hypersensitivity to, and allows more economical use of, 5FOA in Saccharomyces cerevisiae. Yeast. 7:1991;607-608.
20. Mewes H.W., Albermann K., Bahr M., Frishman D., Gleissner A., Hani J., Heumann K., Kleine K., Maierl A., Oliver S.G., Pfeiffer F., Zollner A. Overview of the yeast genome. Nature. 387:1997;7-65.
21. Oliver S.G. From DNA sequence to biological function. Nature. 379:1989;597-600.
22. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning: a Laboratory Manual. second ed. 1989;Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
23. Sauer B. Identification of cryptic lox sites in the yeast genome by selection for cre-mediated chromosome translocations that confer multiple drug resistance. J. Mol. Biol. 223:1992;911-928.
24. Sauer B. Recycling selectable markers in yeast. BioTechniques. 16:1994;1086-1088.
25. Sauer B. Multiplex Cre/lox recombination permits selective site-specific DNA targeting to both a natural and an engineered site in the yeast genome. Nucleic Acids Res. 24:1996;4608-4613.
26. Storici F., Coglievina M., Bruschi C.V. A 2-micron DNA-based marker recycling system for multiple gene disruption in the yeast Saccharomyces cerevisiae. Yeast. 15:1999;271-283.
27. Toh-e A. Construction of a marker gene cassette which is repeatedly usable for gene disruption in yeast. Curr. Genet. 27:1995;293-297.
28. Wach A., Brachat A., Pohlmann R., Philippsen P. New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae. Yeast. 10:1994;1793-1808.
29. Wach A., Brachat A., Alberti-Segui C., Rebischung C., Philippsen P. Heterologous HIS3 marker and GFP reporter modules for PCR-targeting in Saccharomyces cerevisiae. Yeast. 13:1997;1065-1075.
30. Wolfe K.H., Shields D.C. Molecular evidence for an ancient duplication of the entire yeast genome. Nature. 387:1997;708-713.